This workshop will teach the basic concepts and practical aspects of ChIP-Seq data analysis.
Topics will include; experimental approach, quality control, peak calling, and basic biological interpretation.
9:30-10:15 “ChIP-seq considerations”
This section will focus on the importance of experimental design in ChIP-seq.
Topics include; variations on ChIP-seq, inputs, replicates, the limitations of ChIP-seq, and the value of follow-up analyses.
10:30-12:00 “Analysis of ChIP-seq data”
This section will focus on the analytical pipeline that is implemented by CCBR and NCBR. Time will be spent discussing the tools used, why they were chosen, and how to make sense of their outputs. Example results will be shown. Topics include; quality control and read alignment, deduplication, normalization, gene coverage plotting, peak calling, motif analysis, and more.
Date: May 10, 2019
Time: 9:30 AM – 12:30 PM
Location: NIH, Bldg 37, Rm 2041/2107
Presenter(s): Alexei Lobanov (CCBR), Paul Schaughency (NCBR), Tovah Markowitz (NCBR)
Registration link: https://btep.ccr.cancer.gov/classes/chip/
If you are unable to attend in person, webex will be provided.
ChIP-Seq Data Analysis: Probing DNA-Protein Interactions
Hosted by Amy Stonelake
Friday, May 10, 2019 9:30 am | 3 hours | (UTC-05:00) Eastern Time (US & Canada)
Meeting number: 737 294 613
Join by video system
You can also dial 220.127.116.11 and enter your meeting number.
Join by phone
1-650-479-3207 Call-in toll number (US/Canada)
Access code: 737 294 613
Visit the BTEP website: https://btep.ccr.cancer.gov
Questions/Comments and/or suggestions may be directed to: NCIBTEP@mail.nih.gov
Dr. Amy Stonelake 240-760-6314
Dr. Peter FitzGerald 240-760-7181