This workshop will teach the basic concepts and practical aspects of ChIP-Seq data analysis.

Topics will include; experimental approach, quality control, peak calling, and basic biological interpretation.

9:30-10:15 “ChIP-seq considerations”

This section will focus on the importance of experimental design in ChIP-seq.

Topics include; variations on ChIP-seq, inputs, replicates, the limitations of ChIP-seq, and the value of follow-up analyses.

10:15-10:30 Break

10:30-12:00 “Analysis of ChIP-seq data”

This section will focus on the analytical pipeline that is implemented by CCBR and NCBR. Time will be spent discussing the tools used, why they were chosen, and how to make sense of their outputs. Example results will be shown. Topics include; quality control and read alignment, deduplication, normalization, gene coverage plotting, peak calling, motif analysis, and more.

12:00-12:30 Questions

    Date: May 10, 2019

    Time: 9:30 AM – 12:30 PM

    Location: NIH, Bldg 37, Rm 2041/2107

    Presenter(s): Alexei Lobanov (CCBR), Paul Schaughency (NCBR), Tovah Markowitz (NCBR)

Registration link:    https://btep.ccr.cancer.gov/classes/chip/

If you are unable to attend in person, webex will be provided.

ChIP-Seq Data Analysis: Probing DNA-Protein Interactions

Hosted by Amy Stonelake

Friday, May 10, 2019 9:30 am | 3 hours | (UTC-05:00) Eastern Time (US & Canada)

Meeting number: 737 294 613

Password: JwrMe6p@

https://cbiit.webex.com/cbiit/j.php?MTID=m4b85110c7a550bd3815d04ae184f6e1b

Join by video system

Dial 737294613@cbiit.webex.com

You can also dial 173.243.2.68 and enter your meeting number.

Join by phone

1-650-479-3207 Call-in toll number (US/Canada)

Access code: 737 294 613

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 Visit the BTEP website: https://btep.ccr.cancer.gov

 Questions/Comments and/or suggestions may be directed to: NCIBTEP@mail.nih.gov 

 Dr. Amy Stonelake         240-760-6314

Dr. Peter FitzGerald        240-760-7181

Categories: Uncategorized