OSTR | Office of Science and Technology Resources

Next Generation Sequencing Platforms

CCR investigators have access to a variety of DNA sequencing services in both Frederick and Bethesda.

Sequencing and Automation

  • Variety of options for NGS library preparations, including substantial project design consultation, Ion Torrent (CLIA certified), and Illumina MiSeq
  • Whole exome capture using Agilent’s SureSelect reagents
  • 16s Microbiome Pipeline: Automated fecal sample extraction, library preparation, normalization, and sequencing on MiSeq for bacterial 16s RNA gene.
  • Specific single nucleotide polymorphism (SNP) detection and DNA methylation analysis on the Qiagen Pyromark platform
  • Large projects requiring laboratory automation are managed using one of several Beckman BioMek FX liquid handling systems.

For more information contact:
Xiaolin Wu, Ph.D.
301-846-7677
Forestwu@mail.nih.gov

CCR Genomics Core

Platforms available:

  1. Sanger:  ABI 3130XL fluorescence-based sequencer
  2. MiSeq Next Generation Sequencing
  3. NextSeq 500 and 550

This Facility is designed to provide NCI investigators with rapid processing of their DNA sequence samples using Sanger, Illumina MiSeq or NextSeq platforms. The core accepts samples for DNA sequencing or GeneMapper analysis. The facility analyzes these samples using Thermo 3500XL fluorescence-based sequencers.

For projects requiring Next Generation Sequencing, the MiSeq or NextSeq platforms are available.

Requests for MiSeq/NextSeq services should be emailed to E-mail: ncimiseq37@mail.nih.gov

For more information contact:

Elizabeth Conner Ph.D.
Facility Head, CCR Genomics Core
Building 37, Room 2135
37 Convent Drive
Bethesda, MD 20892
301-496-731
 Liz_conner@nih.gov

CCR Sequencing Facility

Click Here to get to the online accessioning system for the SF 

Provides state-of-the-art, high-throughput sequencing technologies to identify single nucleotide polymorphisms, insertions and deletions, and copy number or structural variations.

The SF offers to sequence on two platforms:

Illumina NovaSeq6000, NextSeq500, HiSeq2500, and HiSeq4000 sequencing platforms

  • Sequencing utilizes reversible terminator chemistry optimized to achieve high levels of cost-effectiveness and throughput (Detailed description)
  • Millions of reads produced per sample lane at 36bp and 101bp read lengths
  • Support for the multiplexing of bar-coded samples into a single lane
  • Available resources include one NovaSeq6000, one HiSeq 4000, two NextSeq 500s, and one HiSeq2500 sequencers

PacBio Sequel sequencing

    Long-read sequencing with SMRT Sequencing gives you the ability to:

  • Assemble high-quality de novo genomes
  • Catalog full-length isoforms
  • Unambiguously align sequences
  • Observe fully phased alleles
  • Span repetitive elements and complex regions
  • Resolve structural variants
  • Half of the data in reads >30 kb
  • Output per SMRT cell: up to 10 Gb

Contact information:

Bao Tran
Laboratory Director
ATRF Room D-3047
301-360-3460
301-360-3465
tranb2@mail.nih.gov

Please inquire with the OSTR for information about subsidies for sequencing services.