The Single Cell Analysis Facility (SCAF) is an NCI Center for Cancer Reseach (CCR)-dedicated resource providing support for projects requiring analysis at single cell resolution. We aim to provide end-to-end support from initial experimental design, advice on cell isolation and handling, expertise on existing and emerging single cell technologies, and support on bioinformatic analysis. Current technological expertise includes gene expression profiling of single cells using various platforms and levels of throughput, and we are expanding capabilities to support single-cell genomics, epigenetic, and feature barcoding strategies. We also aim to support emerging single cell technologies, methods, and informatics analysis to provide the most current and relevant support resources to the community.