Rabbit Polyclonal Antibodies and Mouse Monoclonal Antibodies

Rockland Immunochemicals: In 2005, CCR formed a partnership with Rockland Immunochemicals for the development of rabbit polyclonal antibodies against key phospho- and non-phosphoproteins implicated in cancer.

The antibodies are produced by Rockland and validated by CCR investigators.

Over 80 polyclonal antibodies were delivered to CCR investigators at no cost to CCR or the investigators. In 2011 the Rockland/CCR MTA was amended to include the production of mouse monoclonal antibodies at no cost.

For each successful polyclonal or monoclonal antibody produced by Rockland and validated by CCR Rockland will deliver to the validating investigators up to five milligrams of each polyclonal collaboration antibody, three milligrams of each monoclonal collaboration antibody and one vial of Rockland antibodies for validation purposes.

To request an antibody please log into the ABCC/CCR/Rockland file sharing system and click on “Request an Antibody” to complete the form and submit, or contact Karin Heidemann first to discuss your project.

Karin Abarca Heidemann, Ph.D
R&D Director
Rockland Immunochemicals, Inc.
P.O. Box 326, Gilbertsville, PA 19525

Rabbit Monoclonal Antibodies

Abcam (formerly Epitomics, Inc.): In December 2008, the OSTR established a unique partnership with Epitomics Inc. for the development of rabbit monoclonal antibodies.

Rabbit monoclonal antibodies recognize many antigens that are not immunogenic in mice, including small molecules haptens, some cell surface epitopes, modified sites (e.g., phosphorylation, acetylation, methylation, glycosylation, etc.) and most rodent proteins. The antibodies are generally of higher affinity than those from mice. Results obtained from Epitomics and other researchers indicate that the binding affinity of rabbit monoclonal antibodies is on average 50 to 100 times higher, offering better sensitivity and specificity in biological assays. The rabbit system offers 40 times as many lymphocytes compared to the mouse system, the 240E cell line used for fusion is much more robust than any of the mouse cell lines used for fusion and because of the large size of the rabbit spleen, more fusion experiments can be performed.

The cost to the investigator for the development of one custom rabbit monoclonal antibody is $3470 (total cost $6940, 50% OSTR subsidy). Investigators are responsible for validating the antibodies through the production process.

Please download the following documents for detailed information on:

To request an antibody, investigators should contact Sandy Chou (Abcam’s Collaborations Manager), to discuss their protein target for antibody development.  If the antigen is modified, they should specify the exact modification (e.g., phosphorylated, acetylated, methylated, glycosylated, etc.).  Once Abcam agrees to develop the antibody investigators should submit a subsidy request at the OSTR subsidy site.  Please include a Soul Source Justification letter, provided above, with the purchase order.  When placing the order, investigators should charge the full cost of the project to their CAN/Center Number. Subsidy funds will be transferred to the investigator’s CAN/Center Number after each phase of production (see attachment) unless the project is terminated.

Once your project has been initiated, all files and information related to the project is managed through the Abcam/CCR project management website.

Contact information:

Sandy Chou, Ph.D.
Collaboration Manager
Abcam Burlingame
650-583-6688 x2314
Website: Abcam

Antibodies produced by Rockland and Abcam are commercialized 60 to 90 days after completion of bulk production and delivery to the investigators.

For questions regarding the two partnerships please contact Mariam Malik at malikm@mail.nih.gov or 240-760-7183