The CCR Genomics Core in Building 37 provides NCI intramural investigators with a range of genomics technologies, available either as services delivered by the core, or as walk up access to specific instrumentation and technologies.
Manager: Liz A. Conner, Ph.D.
Next-Gen Specialist: Val Bliskovsky, Ph.D.
Fluidigm C1 Access & Training: Liz Conner
Staff: Qin Wei, Ph.D.
Staff: Steve Shema
Address: Building 37, Room 2135, NCI-Bethesda
Core group e-mail: email@example.com
Rapid and accurate sequencing of DNA samples
Expertise in the use of software for sequencing data
Troubleshooting sequencing reactions
Samples are accepted in which sequencing reactions are completed by the user, as well as samples in which the sequencing reaction and clean-up are performed by the core.
Nanostring Digital Gene Expression Analysis
Pathway expression profiling
Validation of complex gene expression signature
Validation of RNA-Seq and array data
Performed directly on total RNA (no RT, IVT, and PCR) or lysates, from a wide variety of sample types including cells, tissues, FFPE, and blood.
Next-Generation Sequencing on Illumina MiSeq and NextSeq500
Cancer panel sequencing
Microbiome sequencing (16S rRNA)
Viral and bacterial genomes sequencing
T-Cell and B-Cell receptor sequencing
Discuss your experimental design with Val Bliskovsky and a group of CCR bioinformaticians. Library preparation may be performed by the Core or by users.
Fluidigm C1 Single Cell Autoprep System for genomic analysis of single cells
An automated single-cell isolation and preparation system that provides an easy and reproducible workflow to process 96 single cells for genomic analysis including:
Whole Genome Amplification and sequencing
Gene Expression Profiling on Biomark HD qPCR (available through LMT in Frederick)
Contact Liz Conner to schedule training and access to the instrument. The core also provides access to the Nexcelom Vision CBA image cytometry system to enable characterization of sample prep, including cell size, number and viability prior to single cell isolation on the C1.
Bio-Rad QX200 Droplet Digital PCR System
Absolute quantification of target DNA, including measurement of viral loads and microbial quantification.
Detection of copy number variations and rare mutations, especially in wildtype background.
Absolute quantification of mRNA and miRNA, especially low species.
Quantification of NGS libraries.
Other Available Technologies
- Agilent BioAnalyzer
- Agilent TapeStation
- Qubit Fluorometric Quantitation