NATIONAL CANCER INSTITUTE - CANCER.GOV

Contact Information

Primary Contact

Kristen Pike
Associate Scientist II

Location

8560 Progress Drive
Frederick, Maryland 21701

Overview

CLIA-Certified Technologies Offered:

  • Fragment Analysis for Micro-satellite Instability Detection
  • Pharmacoscan Array for Pharmacogenomics
  • Mutation Detection for PCR and Sanger Sequencing
  • DNA extraction from whole blood, saliva, FFPE tissues, buccal swabs, nails, hair, PBMCs, buffy coats, plasma, tissue slides, cell lines, frozen tissues, and platelet-depleted whole blood
  • Droplet Digital PCR (ddPCR)
  • Mass Spectrometry

Additional Capabilities Include:

  • Quantitative PCR (qPCR)
  • Next-Generation Sequencing (NGS)
  • Automated Staining (RNAscope)
  • GeneChip Array Plate assays for Gene Titan

Major Instrumentation

  • SeqStudio 24 Flex Genetic Analyzer
  • NovaSeq6000 Sequencing System
  • Illumina MiSeqDx for Next-Generation Sequencing (NGS)
  • Leica BondRx stainer for RNAScope assays
  • Qiagen QIAcuity One Digital PCR System 5plex Instrument
  • Thermo Fisher QuantStudio for qPCR
  • Illumina NextSeq2000 for Next-Generation Sequencing (NGS)
  • BioRad QX200 for Droplet Digital PCR (ddPCR)
  • Beckman FXp robotic liquid handler for automation
  • Microm Microtome for sectioning formalin fixed paraffin embedded (FFPE) tissues

User Guidelines

NIH Investigators may place a formal request for our lab's services at https://ncifrederick.cancer.gov/services/accessioning/Home/SignIn?ReturnUrl=%2fservices%2faccessioning%2f..

Publications

  • Frameshift mutations in peripheral blood as a biomarker for surveillance of Lynch syndrome. https://doi.org/10.1093/jnci/djae060. Yurong Song, Holli Loomans-Kropp, Ryan N Baugher, Brandon Somerville, Shaneen S Baxter, Travis D Kerr, Teri M Plona, Stephanie D Mellott, Todd B Young, Heidi E Lawhorn, Lei Wei, Qiang Hu, Song Liu, Alan Hutson, Ligia Pinto, John D Potter, Shizuko Sei, Ozkan Gelincik, Steven M Lipkin, Johannes Gebert, Matthias Kloor, Robert H Shoemaker. JNCI: Journal of the National Cancer Institute, Volume 116, Issue 6, Pages 957u2013965. Published: 11 March 2024
  • Organoids and metastatic orthotopic mouse model for mismatch repair-deficient colorectal cancer. https://doi.org/10.3389/fonc.2023.1223915. Yurong Song, Travis D. Kerr, Chelsea Sanders, Lisheng Dai, Shaneen S. Baxter, Brandon Somerville, Ryan N. Baugher, Stephanie D. Mellott, Todd B. Young, Heidi E. Lawhorn, Teri M. Plona, Bingfang Xu, Lei Wei, Qiang Hu, Song Liu, Alan Hutson, Baktiar Karim, Sandra Burkett, Simone Difilippantonio, Ligia Pinto, Johannes Gebert, Matthias Kloor, Steven M. Lipkin, Shizuko Sei and Robert H. Shoemaker. Frontiers in Oncology 2023, 13, 1-17. Published: 08 September 2023.
  • Mesothelioma Mouse Models with Mixed Genomic States of Chromosome and Microsatellite Instability. https://doi.org/10.3390/cancers14133108. Yurong Song, Shaneen S. Baxter , Lisheng Dai, Chelsea Sanders, Sandra Burkett, Ryan N. Baugher , Stephanie D. Mellott, Todd B. Young, Heidi E. Lawhorn, Simone Difilippantonio, Baktiar Karim, Yuwaraj Kadariya, Ligia A. Pinto, Joseph R. Testa and Robert H. Shoemaker. Cancers 2022, 14, 3108. Published: 24 June 2022
  • Kajal Biswas, Martin Couillard, Luca Cavallone, Sandra Burkett, Stacey Stauffer, Betty Martin, Eileen Southon, Susan Reid, Teri Plona, Ryan Baugher, Stephanie Mellott, Kristen Pike, Mary Albaugh, Chelsea Maedler-Kron, Nancy Hamel, Lino Tessarollo, Victoria Marcus, William Foulkes, and Shyam Sharan: A Novel Mouse Model of PMS2 Founder Mutation that Causes Mismatch Repair Defect Due to Aberrant Splicing. Cell Death & Disease, DOI 10.1038/s41419-021-04130-8 Paper #CDDIS-21-1697RR], September 6, 2021.
  • Tristan M. Sissung, Roberto H. Barbier, Douglas K. Price, Teri M. Plona, Kristen M. Pike, Stephanie D. Mellott, Ryan N. Baugher, Gordon R. Whiteley, Daniel R. Soppet, David Venzon, Arlene Berman, Arun Rajan, Giuseppe Giaccone, Paul Meltzer, and William D. Figg: Comparison of Eight Technologies to Determine Genotype at the UGT1A1 (TA)n Repeat Polymorphism: Potential Clinical Consequences of Genotyping Errors? International Journal of Molecular Sciences, 2020.

Keywords

ACD geneACVR2AAIM2AKT1 geneAPCAPOL1 geneASTE1ATR geneBAK1 geneBHD Exons 1-14BRAF gene Exons 11 & 15BRCA1 geneBRCA2 geneCARD 15 (NOD2) Exons 1-12CARD9CD18 gene Exons 1-16CDK4 geneCDKN2A gene Exons 1-3 & promoter regionCLIACLIC1CXCR4 Exons 1-2CYBA (p22phox) Exons 1-6CYBB(gp91phox) Exons 1-13CYBC1DAMSDEFB1 geneDEFB103 geneDKC1 geneDNA extractionDectin 2 (CLEC6A)Dectin-1 gene (CLEC7A)ELANE exons 1-5FBXW7 geneFH Exons 1-10FoxP3 Exons 1-12G6PDG6pc3 geneH-ras exons 2-5HAX1 geneIDH1 geneIL8RB (CXCR2) Exons 1-3IRAK4 geneJAGN1 Exons 1K-ras exons 2-5K-ras gene codon 12LIMK2 geneLMAN1LSTMARCKSMC1R geneMITF Exons 1-13MPO geneMSHMSH2 geneMSH3MTAP Exons 1-8N-ras exons 2-5NCF1 (p47phox) Exons 1-11NCF2 (p67phox) Exons 1-16NCF4NF1 geneNOLA2 geneNOLA3 geneP14 geneP53 (human) genePADI4PARP1 genePARP2 genePIK3CA genePOT1 genePTCH1PTEN geneRNASET2RNAScopeRNF43SMAD4 geneSTMN1 Exons 1-4Sanger SequencingTAF1BTCF4TEP1 geneTERC geneTERF1 geneTERF2 geneTERF2IP geneTERT geneTGFBR2TINF2 geneTPP1 geneUGT1A1VHL gene Exons 1-3WDR1 geneanalysisClinical Research Supportmass specmass spectrometrymutation detectionnci-coreqPCRPharmacoscanfragment analysisPCRddPCRDroplet digital PCRNGS (next generation sequencingpharmacogenomicsRNA extractionbloodbuffy coatplasmaFFPEbuccal swabsalivaCYP3A4*6EPAS1 genemBAT30mBAT37mBAT59mBAT64mBAT67mL24372mPMS2mU12235RUNX1TSPOplatelet-depleted whole bloodDNA extraction from hair and nails