Established and emerging technologies are needed by CCR investigators in order to systematically dissect the heterogeneity of cancer in order to gain a better understanding of the causes of invasion, metastasis, and therapy resistance. These coveted technologies have been identified as having the strongest footing among the leading institutions around the world that are focused on Single Cell (SC) genomic analysis. The OSTR is dedicated to providing these groundbreaking technologies to CCR investigators through educational and informational resources designed to introduce users to various scientific methodologies, as well as seminars on current advancements in scientific methods and instrumentation.
OSTR VIDEO LIBRARY
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Technology Seminars
A collection of technology presentations given by scientists outside of CCR, either other programs at NIH or the biotechnology sector. The technologies presented in these videos are made available to all CCR investigators through core facilities or other laboratories at NIH.
Nanopore Sequencing for Single cell and Spatial Applications
Oxford Nanopore Technologies and 10X Genomics provide updates on using long read sequencing for single cell and spatial applications.
Adaptive ImmunoSeq
Adaptive ImmunoSeq TCR Sequencing specifically as it relates to accelerating discovery and innovation.
10X Platforms
Pushing the boundaries of Single Cell with Chromium X
RNAscope
Detecting single RNA molecule at single cell resolution in intact tissue, with unique applications in gene therapy, cell therapy, point mutation, and sc RNAseq validation.
Center for Advanced Tissue Imaging (CAT-I)
This seminar will detail IBEX, RAPID, Ce3D, and other technologies, show examples of their application to various tissues and cancers, and explain how CAT-I is structured to enable access to these methods by CCR investigators
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Core Spotlight Seminars
A series of seminars highlighting the resources offered by various NIH Core facilities. Each seminar features an overview of the Core’s capabilities, followed by a testimonial showcasing how the Core supported a specific research project.
Animal Research Services
NIH ORS and NIH CREx Panel Discussion Series. Panel 2: Animal Model Research Services. February 24, 2022.
Core Spotlight Seminar
Christopher Bleck, Ph.D., Director, NHLBI Electron Microscopy Core
“Biology Goes 3D: Volume Electron Microscopy of Biological Specimens”
Core Spotlight Seminar
Dillon Chung, Ph.D., NHLBI Laboratory of Cardiac Energetics at NHLBI
“Mitochondrial Network Formation as a Tissue-Specific Phenomenon in a Mammalian Model of Extreme Metabolism
Core Spotlight Seminar
Raymond Fields, Director, NINDS Viral Production Core
“An Introduction to Viral Vectors and the NINDS Viral Production Core Facility”
Core Spotlight Seminar
Emily Petrus, Ph.D., NINDS Laboratory of Functional and Molecular Imaging
“Viral Enabled Study of the Neurons and Circuits Underlying the Brain’s Response to Peripheral Injury”
Core Services: Mass Spectrometry
NIH ORS and NIH Collaborative Research Exchange (CREx) Panel Discussion Series. Panel 1. October 27, 2021
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Technology Educational Videos
A collection of short technology presentations given by CCR-dedicated facility managers. These videos offer CCR investigators insights into various advanced technologies made available to support CCR research.
Protein Mass Spectrometry
Mass spectrometry (MS) analysis of proteins measures the mass-to-charge ratio of ions to identify and quantify molecules in simple and complex mixtures. MS has become invaluable across a broad range of fields and applications, including proteomics. The development of high-throughput and quantitative MS proteomics workflows within the last two decades has expanded the scope of what we know about protein structure, function, modification and global protein dynamics. This overview outlines the role of mass spectrometry in the field of proteomics, reviews MS methodology and instrumentation, and touches on sample preparation and liquid chromatography–based separation prior to MS analysis.
Proteomics Analysis using Mass Spectrometry. Structural Mass Spectrometry related to crosslinking and limited proteolysis mass spectrometry approaches.
Quantitative mass spectrometry, specifically the global discovery experiments in mass spectrometry. PTM mass spectrometry, specifically the analysis of post-translational modifications by mass spectrometry. Interactome mass spectrometry approaches specifically, proximity-dependent biotinylation for mapping protein complexes mass spectrometry. Structural mass spectrometry approaches specifically, Hydrogen/Deuterium Exchange mass spectrometry.
Molecular Microscopy
Electron microscopy (EM) has been a long-standing tool in the ultrastructural analysis of cells and tissues. Over the last 3 decades, it has also evolved into a powerful technique for the structural study of biological macromolecules. The main difference between this molecular EM and the more conventional EM of fixed tissue sections is its ability to deliver three-dimensional (3D) structures of the studied complexes at the higher resolution necessary to visualize structural details of molecules (on the scale of nanometers) rather than of the gross architecture of cells (on the scale of micrometers). Whereas modern electron microscopes can routinely deliver images of inorganic material at atomic resolution, biological specimens pose great difficulties for EM imaging, significantly reducing the attainable resolution.
An introduction to focused ion beam scanning electron microscopy (FIB-SEM) as it relates to visualizing cells in 3-D and at the nanoscale level.