NATIONAL CANCER INSTITUTE - CANCER.GOV

Contact Information


Primary Contact

Milind Pore, Ph.D.
Scientist

Location

8560 Progress Drive
Federick, MD 21701

Additional Contacts

Donald Paul Mallory, Ph.D.
Data Scientist
William Bocik, Ph.D
Program Manager

Overview

The Mass Cytometry Core (MCC) provides access to cutting-edge platforms and expert technical support for high-dimensional single-cell analysis and spatial proteomic profiling. A central expertise of the core is Imaging Mass Cytometry (IMC), which allows simultaneous quantification of dozens of protein markers in intact tissue sections at single-cell resolution while preserving spatial context. This approach enables in-depth analysis of tissue architecture, micro-environments, and cellular interactions. In addition, the MCC provides suspension mass cytometry (CyTOF) for high-parameter profiling of dissociated cells, supporting comprehensive phenotypic and functional analysis of complex cellular populations. Together, these complementary technologies facilitate detailed investigation of cellular heterogeneity and spatial organization, providing powerful insights into complex biological systems and disease processes.

Core Services Include:

  • design and procurement of application-specific antibody panels,
  • in-house metal conjugation of antibodies,
  • panel validation and sample staining for CyTOF and IMC studies, and
  • data acquisition and downstream data analysis using the Bodenmiller Lab pipeline.

The core also offers validated marker panels spanning key biological domains, including epithelial-to-mesenchymal transition, metabolic pathways, tumor microenvironment characterization, cancer type–specific protein markers, proliferation, and cancer stem cell biology.

User Guidelines

Core is open for CCR investigators. Support for non-NCI investigators is dependent on the capacity of the Core.

Keywords

Mass CytometryNCICyTOFimaging mass cytometry (IMC)Hyperion XTsingle-cell proteomic profiling