NATIONAL CANCER INSTITUTE - CANCER.GOV

Contact Information

Primary Contact

Rick Huang, PhD
Facility Manager

Location

37 Convent Dr.
Building 37, Room 3035
Bethesda, MD 20892

Additional Contacts

Allison Lupatkin
Scientist
Haotian Lei, PhD
Facility Manager

Overview

NIH Intramural CryoEM Consortium (NICE) serves intramural investigators in all NIH IC’s. NICE provides access to state-of-the-art Titan Krios cryo-electron microscopes for atomic-resolution structure determination of protein, macromolecular complexes, membrane receptors, cellular organelles, and infectious pathogens, using single particle or cryo-tomography approaches. Fine detailed 3D maps computed by electron micrographs spark numerous biological insights in cancer research, infectious disease research, vaccine design, cell development, and human physiological and functional studies.
Microscopy Applications
Negative Staining and cryoEM sample preparation.
Negative Staining and cryoEM sample screening.
CryoEM Single Particle and tomography data collection.

Major Instrumentation

Building 37
Titan Krios 300kV Electron Microscope with Gatan BioQuantum Image Filter and K3 Direct Electron Detector
FEI Vitrobot mach4
Leica Cryo GP2
Ted Pella Easy Glow grid discharging system

Building 33
Glacios 200kV Electron Microscope with Selectris Image Filter and Falcon 4i Direct Electron Detector

Contact: Dr. Haotian Lei

Building 50
Tecnai 12 120kV Electron Microscope with K2 summit
2 Gatan 626 cryo holders
1 multi-specimen cryo holder
Gatan turbo pumping stations for cryo holders

User Guidelines

NICE is a resource available to intramural investigators in NCI, NIAID, NIEHS, NICHD, NIDCR, NEI, and NIA.

Keywords

Cryo Electron microscopyEMcryoEMcryoEM single particleelectron microscopeChemistry and Structural Biologynci-corenegative stainingnih-coretomography