Rockville, MD
Core Facility
Trans NIH Facility
The Functional Genomics Laboratory (formerly, the RNAi Screening Facility) of the National Center for Advancing Translational Sciences (NCATS) assist investigators with all stages of project planning and execution, beginning with assay development through genome-wide siRNA Read More...
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Bioinformatics
03/12/2013 - Using pathway analysis in MetaCore/GeneGO to evaluate glioma subtypes from public gene expression data MetaCore from GeneGo is an integrated software suite for functional analysis of microarray, metabolic, SAGE, proteomics, siRNA, microRNA, and Read More...
Bethesda, MD
Collaborative
The NCI High-Throughput Imaging Facility (HiTIF) works in a collaborative fashion with NCI/NIH Investigators by providing them with the necessary expertise, instrumentation, and software to develop and execute advanced High-Throughput Imaging (HTI) assays. These Read More...
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Confocal
HiTIF Protocols Cell Fixation PFA Cell Fixation with the Blue Washer Cell Seeding Seeding Cells in the imaging plates using the Multidrop Combi Functional Genomic Screens RNAi/CRISPR-KO Screen Assay Development SOP siRNA/sgRNA Spotting Read More...
Rockville, MD
Trans NIH Facility
The Chemistry and Synthesis Center (CSC) of the National Heart, Lung, and Blood Institute (NHLBI) provides IRP scientists with targeted imaging probes and chemical tools that help accelerate cell-based assays, in vivo imaging studies, and Read More...
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Confocal
A typical HTI screening project at HiTIF can be subdivided in 5 phases: Pre-Development The investigator contacts the Facility Head to inquire about the current availability of instrumentation time and manpower for the project to be Read More...
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Confocal
Yokogawa CV8000 High-throughput spinning disk confocal microscope The Yokogawa CV8000 is HiTIF second high-throughput imaging system. The CV8000 feature set is similar to the CV7000.When compared to the latter, the CV8000 has a newer Read More...