Single Cell Analysis Facility (SCAF)
The rapid advancement of single-cell technology has provided new powerful tools to answer many biological questions, such as identifying new or rare cell populations and characterizing the complexities of tumor heterogeneity. Realizing the great potential of single-cell technology in cancer research, the CCR has established a new Single Cell Analysis Facility (SCAF) located on the Bethesda main campus.
The SCAF works closely with CCR Sequencing Facility to also extend support to CCR Investigators located in Frederick, and works with other associated support groups at CCR, including CCR Genomics Core in Building 37, CCR Genomics Technology Lab, CCR Collaborative Protein Technology Resource in Building 37, the CCR Bioinformatics Training and Education Program, and the CCR Collaborative Bioinformatics Resource. SCAF aims to provide the broadest range of project support from consultation on experimental design sequencing and data analysis.
The SCAF provides the most advanced single-cell genomics technologies to CCR investigators, including many established platforms, such as:
- 10X Genomics Chromium system
- Advanced Methods: Plate-based single cell approaches (e.g., Smart-Seq2)
Emerging technologies include:
- 10x Genomics Visium
- 10x Genomics Feature Barcoding
- Combinatorial Indexing Single Cell approaches
- 10X Genomics Xenium Analyzer
These technologies give CCR-investigators the options for single cells studies, ranging from a few cells to hundreds of thousands of cells, and from single cell genomic assays such as RNA-seq to single cell protein quantification. Both locations have dedicated bioinformatics staff to help you perform preliminary analysis and deliver the data in a format that will enable to focus on your biological questions.
These technologies give CCR-investigators the options for single cells studies, ranging from a few cells to hundreds of thousands of cells, and from single cell genomic assays such as RNA-seq to single cell protein quantification.
For general information about single-cell genomics technologies available to CCR, please contact Dr. Mike Kelly michael.kelly3@nih.gov.
For general information and project consultation using the CODEX or NanoString technology, please see the CPTR webpage Collaborative Protein Technology Resource (CPTR).
To initiate a project consultation or schedule an experiment with our experts, please contact:
CCR Single Cell Analysis Facility (SCAF) Contact:
(For Investigators Located in Bethesda):
Dr. Mike Kelly (please ‘cc’ our group email)
michael.kelly3@nih.gov
CCR-SCAF@nih.gov
(For Investigators Located in Frederick):
Your Project Will Continue to Be Supported by CCR Sequencing Facility (SF)
CCR Sequencing Facility (SF)