Genetics and Genomics

Our operational objectives are to provide state-of-the-art OMICS technologies in support of the Genetics Branch (GB) investigators and collaborators. Research Services: Wet Lab • Single cell isolation from fresh, frozen and FFPE tissue • DNA/RNA extractions from fresh, frozen, FFPE tissue, and tissues preserved by other methods • Cell free circulating DNA from plasma and serum • Quality assessment of nucleic acids by spectrophotometry, fluorometry, qPCR, capillary electrophoresis, digital droplet PCR • Next-generation sequencing (NGS) library preps • Sequencing (Whole … [learn more]

The NCI High-Throughput Imaging Facility (HiTIF) works in a collaborative fashion with NCI/NIH Investigators by providing them with the necessary expertise, instrumentation, and software to develop and execute advanced High-Throughput Imaging (HTI) assays. These can be paired to screen libraries of RNAi or CRISPR/Cas9 reagents to discover and characterize novel cellular pathways by functional genomics. In addition to functional genomics screens, HiTIF also develops HTI assays to extract and quantify single-cell information about the molecular mechanisms underlying rare or heterogeneous b… [learn more]

CIP Microbiome and Genetics Core: The Cancer and Inflammation Program – Microbiome and Genetics Core (CIP-MGC) grew out of the former CIP Genetics Core to meet the increasing need for sequencing and analysis of commensal microbiota within CIP and NCI. The MGC team specializes in: - sample handling, purification and 16S sequencing - bioinformatic processing and computational analysis of sequenced reads - support in genetics and statistical epidemiology Established Technologies: We use the Eppendorf epmotion 5073 and 5075 robots for the normalization of sample concentrations and s… [learn more]

NCI LASP Genome Modification Core (GMC) is a CCR-dedicated facility that provides advice, training, and reagents to NCI scientists seeking to utilize CRISPR and other nucleases to generate genome modifications in primary cells, cell lines, and in animal models. Established Technologies : We are currently offering a variety of services to CCR investigators. Moreover, we also have accumulated many different flavors of genome editing reagents. Here is a brief summary of what we are currently offering: Consulting – if you are new to these areas of research and wish to find out more … [learn more]

The Pan-Microbial Serology Facility (PMSF) is part of the Center for Cancer Research (CCR) at the National Cancer Institute (NCI). The PMSF focuses on determining individualized pan-microbial immune profiles associated with human diseases including immunological disorders such as cancer by providing a standardized intramural technological portal. Throughout our lives, we are exposed to various microbes including viruses, creating an individualized microbial landscape. Each microbe infection can leave unique immunological footprints, known as antigen-specific antibodies, which result in a pers… [learn more]