Genetics and Genomics

Our operational objectives are to provide state-of-the-art OMICS technologies in support of the Genetics Branch (GB) investigators and collaborators. Research Services: Wet Lab • Single cell isolation from fresh, frozen and FFPE tissue • DNA/RNA extractions from fresh, frozen, FFPE tissue, and tissues preserved by other methods • Cell free circulating DNA from plasma and serum • Quality assessment of nucleic acids by spectrophotometry, fluorometry, qPCR, capillary electrophoresis, digital droplet PCR • Next-generation sequencing (NGS) library preps • Sequencing (Whole … [learn more]

The NCI High-Throughput Imaging Facility (HiTIF) works in a collaborative fashion with NCI/NIH Investigators by providing them with the necessary expertise, instrumentation, and software to develop and execute advanced High-Throughput Imaging (HTI) assays. These can be paired to screen libraries of RNAi or CRISPR/Cas9 reagents to discover and characterize novel cellular pathways by functional genomics. In addition to functional genomics screens, HiTIF also develops HTI assays to extract and quantify single-cell information about the molecular mechanisms underlying rare or heterogeneous b… [learn more]

The Pan-Microbial Serology Facility (PMSF) is part of the Center for Cancer Research (CCR) at the National Cancer Institute (NCI). The PMSF focuses on determining individualized pan-microbial immune profiles associated with human diseases including immunological disorders such as cancer by providing a standardized intramural technological portal. Throughout our lives, we are exposed to various microbes including viruses, creating an individualized microbial landscape. Each microbe infection can leave unique immunological footprints, known as antigen-specific antibodies, which result in a pers… [learn more]