NATIONAL CANCER INSTITUTE - CANCER.GOV

Contact Information

Primary Contact

Ross Lake
Manager

Location

Building 37, Room 1066
Bethesda, MD 20892

Overview

The LCBG Microscopy Core offers imaging technologies and training. The Core has established instrumentation for for 2D and 3D imaging of both fixed and living specimens. Equipment include: Zeiss AxioObserver.Z1 epifluorescent microscope, Zeiss AxioScan.Z1 slide scanner, Zeiss LSM780 confocal microscope, and Nikon Eclipse Ti2 widefield microscope.

Major Instrumentation

Zeiss AxioObserver.Z1 epifluorescent microscope

  • Inverted microscope
  • Colibri 7 LED source with 385nm, 430nm, 475nm, 511nm, 555nm, 590nm and 630nm LEDs
  • Filter cubes for DAPI, CFP, YFP, GFP, Cy3, mCherry, and Cy5
  • Hamamatsu Orca Flash4.0 V3 sCMOS Camera
  • 5x, 10x, 20x, 40x, and 63x objective lenses
  • Motorized scanning stage
  • Carl Zeiss Zen Blue 3.9 Acquisition software

Zeiss Axio Scan.Z1 slide scanning microscope

  • Fully automated slide scanning microscope able to create brightfield and fluorescent virtual slides
  • Colibri 7 LED source with 385nm, 430nm, 475nm, 555nm, 590nm and 630nm, and 730nm LEDs
  • Filter cubes for 7-color simultaneous imaging of DAPI, CFP, GFP, Cy3, mCherry, Cy5, and Cy7
  • 2.5x, 5x, 10x, 20x, and 40x objective lenses
  • Up to 100 1×3 or 50 2×3 slides can be imaged in a single experiment
  • Carl Zeiss Zen Blue 3.7 Acquisition software

Zeiss LSM 780 confocal microscope

  • Inverted microscope
  • Two PMTs and one 32 channel GaAsP spectral detector for confocal imaging
  • 10x, 20x, 40x, 63x, and 100x objective lenses
  • Lasers: Argon (458, 488, and 514nm), Diode lasers (405, 561, 594nm), and HeNe laser (633nm)
  • Motorized scanning stage
  • Stage top incubator for live cell imaging
  • Carl Zeiss Zen Black 2012 Acquisition software
  • Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Loss
    in Photobleach (FLIP) to study dynamics of molecular mobility

Nikon Eclipse Ti2 widefield microscope

  • Inverted microscope
  • Lumencor SOLA SE 365 FISH light engine
  • Filter cubes for FISH imaging DAPI, Aqua, Green2, Gold, Red2, and Cy5
  • Photometrics Prime BSI sCMOS camera
  • 4x, 10x, 20x, 40x, 60x and 100x objective lenses
  • Motorized scanning stage with Z piezo insert for fast Z stack acquisition
  • Tokai Hit stage top incubator for live cell imaging
  • Nikon Elements Acquisition software

User Guidelines

The Core is open to all NCI intramural researchers, although LCBG scientists have priority use of the equipment in the facility. Researchers from other institutes are also welcome to use the Core for a nominal fee. Core staff provide individual training for all new users. Unless special arrangements are made, the equipment in the facility may be used Monday to Friday, 10:00 am – 6:00 pm

Keywords

2D and 3D imaging of live and fixed specimensZeiss AxioObserver.Z1 Epifluorescent MicroscopeZeiss LSM 780automated slide scannerconfocal microscopyImaging and Microscopylive cell imagingmicroscopynci-coreoptical microscopy