NATIONAL CANCER INSTITUTE - CANCER.GOV

Contact Information


Primary Contact

Ross Lake
Manager

Location

Building 37, Room 1066
Bethesda, MD 20892

Overview

The Laboratory of Cancer Biology and Genetics (LCBG) Microscopy Core provides state-of-the-art microscopy and training to LCBG investigators, as well as scientists from other laboratories in the CCR. The mission of the LCBG Microscopy Core is to support high quality clinical and translational research in cancer biology by training investigators in the field of confocal and light microscopy. The Core is open to all CCR intramural researchers. Core staff provides individual training for all new users, and can consult with experimental design, specimen preparation and staining, image acquisition using the appropriate equipment, and image analysis. The goal is to provides state-of-the-art equipment, materials, and expertise to investigators in the CCR to help advance their cancer research. There is no charge to CCR investigators for Core access. Researchers from other institutes are welcome to use the facility for a fee.

Major Instrumentation

Zeiss AxioObserver.Z1 epifluorescent microscope

  • Inverted microscope
  • Colibri 7 LED source with 385nm, 430nm, 475nm, 511nm, 555nm, 590nm and 630nm LEDs
  • Filter cubes for DAPI, CFP, YFP, GFP, Cy3, mCherry, and Cy5
  • Hamamatsu Orca Flash4.0 V3 sCMOS Camera
  • 5x, 10x, 20x, 40x, and 63x objective lenses
  • Motorized scanning stage
  • Carl Zeiss Zen Blue 3.9 Acquisition software

Three Zeiss Axio Scan.Z1 slide scanning microscope

  • Fully automated slide scanning microscope able to create brightfield and fluorescent virtual slides
  • Colibri 7 LED source with 385nm, 430nm, 475nm, 555nm, 590nm and 630nm, and 730nm LEDs
  • Filter cubes for 7-color simultaneous imaging of DAPI, CFP, GFP, Cy3, mCherry, Cy5, and Cy7
  • 2.5x, 5x, 10x, 20x, and 40x objective lenses
  • Up to 100 1×3 or 50 2×3 slides can be imaged in a single experiment
  • Carl Zeiss Zen Blue 3.7 Acquisition software

Zeiss LSM 980 Airyscan 2 confocal microscope

  • Inverted microscope
  • Two PMTs and four GaAsP detectors for confocal imaging
  • 4 color Airyscan 2 super-resolution detector
  • Airyscan Multiplex modes for increased acquisition speed with high sensitivity
  • 5x, 10x, 20x, 40x, 63x objective lenses
  • 405nm, 445nm, 488nm, 514nm, 561nm, 594nm, 639nm Diode lasers
  • Environmental chamber for heating and cooling
  • High-resolution motorized scanning stage with Z-piezo insert for fast Z-stack acquisition
  • Carl Zeiss Zen Blue 3.13 Acquisition software

    Nikon Eclipse Ti2 widefield microscope

    • Inverted microscope
    • Lumencor SOLA SE 365 FISH light engine
    • Filter cubes for FISH imaging DAPI, Aqua, Green2, Gold, Red2, and Cy5
    • Photometrics Prime BSI sCMOS camera
    • 4x, 10x, 20x, 40x, 60x and 100x objective lenses
    • Motorized scanning stage with Z piezo insert for fast Z stack acquisition
    • Tokai Hit stage top incubator for live cell imaging
    • Nikon Elements Acquisition software

    User Guidelines

    The LCBG Microscopy Core is open to all CCR intramural researchers. There is no charge to individual users for microscope time. Collaborations with laboratories outside the CCR are also considered, time permitting and fee for service. The Core is staffed Monday to Friday, 10:00 am – 6:00 pm. Our policies for independent use of the equipment during evening and weekends will be determined by the core staff depending upon the level of user expertise.

    Acknowledgement or co-authorship depending on the level of input from the Core staff is requested to support the Core research contribution and to ensure proper funding is justified for the Core to continue adding more capabilities and cutting-edge technology.

    Keywords

    2D and 3D imaging of live and fixed specimensautomated slide scannerconfocal microscopyImaging and Microscopylive cell imagingmicroscopyoptical microscopyAxioscanWidefieldAiryscanMultiplexAxio ObserverSuper ResolutionFRAPbrightfield slide scannerfluorescence slide scannerZeiss LSM 980super-resolution fluorescence microscopyimmunofluorescencenci coreccr core