The EML offers investigators access to unique expertise and EM technologies that allow CCR Investigators to explore new avenues of research in order to enhance the knowledge of biological systems. To assist our customers, we provide complete specimen preparation service which includes shipment of fixative, processing, embedding, sectioning, and imaging of samples. If desired, investigators can also discuss the design of custom protocols tailored to their specific needs. EML is operated by Leidos Biomedial Research Inc. on behalf of NCI as part of the Frederick National Laboratory.Contact … [learn more]

LCMB Microscopy Core offers live cell imaging technologies as well as standard confocal systems for immunofluorescence. Our confocal instruments are a Leica SP8 LSCM and a spinning disk confocal microscope. A Total Internal Reflection Fluorescence (TIRF) microscope is also available and Single Molecule Localization Microscopy can be performed on this system. Contact Details Manager: Sandeep Pallikkuth, Ph.D. Phone: 240.760.6360 Email: sandeep.pallikkuth@nih.gov Address: Building 37, Room 2033B, Bethesda Established Technologies Live cell imaging… [learn more]

LGCP Microscopy Core offers imaging technologies and training. The facility has established instrumentation for for 2D and 3D imaging of both fixed and living specimens. Equipment include: Zeiss AxioObserver.Z1 epifluorescent microscope, Zeiss AxioScan.Z1 slide scanner, Zeiss LSM780 confocal microscope, and Nikon Eclipse Ti2 widefield microscope. Contact Details Manager: Ross Lake Phone: 240-760-6824 Email: laker@mail.nih.gov Address: Building 37, Room 1066, Bethesda Established Technologies Zeiss AxioObserver.Z1 epifluorescent microscope 2D and 3D imaging of live and fixed … [learn more]

The core provides access to several different state-of-the-art 3D microscopes as well as computers to visualize and process image data. The facility houses equipment for 2D or 3D imaging of fixed and living specimens. High resolution images can be obtained by confocal microscopy or deconvolution, and super-resolution techniques SIM, STORM, MINFLUX. Single Molecule tracking is supported on MINFLUX, and custom HILO-illumination microscopes. Contact Details Manager: Tatiana Karpova, Ph.D. Phone: 240.760.6637 Email: karpovat@mail.nih.gov Address: Building 41, Roo… [learn more]

The function of the SAIP is to collaborate with NCI investigators in the development of mouse models, new molecular imaging probes for early detection and therapy, monitor tumors in vivo, and perform drug efficacy studies utilizing in vivo imaging techniques. In addition, the SAIP collaborates with the NCI Division of Cancer Treatment and Diagnosis (DCTD) Nanotechnology Characterization Laboratory (NCL) to analyze nanoplatforms as part of the cascade assay and assist DCTD initiatives in developing standards in small animal imaging, integrate imaging into drug development, and develop par… [learn more]

OMAL focuses its research and development activities to quantitatively understand the molecular basis of three-dimensional (3D) cell organization, motility, invasion, and differentiation using fixed samples and live, 3D tissue culture models (i.e., translational models). OMAL is a CCR-dedicated facility. Prospective users and collaborators should first consult the following website before contacting OMAL staff: https://confocal.ccr.cancer.gov/nci-microscopy-core-labs/frederick/omal/.OMAL is operated by Leidos Biomedial Research Inc. on behalf of NCI as part of the Frederick National Labo… [learn more]

The NHLBI Murine Phenotyping Core carries out physiologic and behavioral testing in a diversity of mouse models for NHLBI and other NIH institutes.Established TechnologiesCardiovascular PhenotypingMetabolic PhenotypingPulmonary PhenotypingBehavioral PhenotypingExercise PhysiologyEchocardiographyIn vivo functional vascular phenotypingAdvanced Imaging Modalities (whole body high frequency ultrasound imaging, vascular imaging) [learn more]

The CCR Microscopy Core provides NCI investigators access to state-of-the-art imaging tools and techniques, including light sheet fluorescence, high-resolution confocal, multi-photon, and super-resolution microscopy. The mission of the CCR Microscopy Core Facility is to support the microscopy and digital imaging needs of investigators studying the biological structures and cellular processes involved in the cell biology of cancer by applying our diverse imaging resources, expertise, and quality customer service. The goal is to provide cutting-edge imaging technology, expert consultation … [learn more]

The Frederick National Laboratory for Cancer Research (FNLCR) Molecular Histopathology Laboratory (MHL) services the NIH communities in Bethesda and Frederick, Maryland. Established Technologies Anatomic Pathology Toxicologic Pathology Mouse Embryology Genetically Engineered Mouse Phenotyping Hematology and Clinical Chemistry (non-human) Tissue Embedding/Block Preparation (paraffin, OCT, Resin) Tissue Microarray Production Microtomy (paraffin, frozen, vibratome). Serial and step sectioning. Histological Stain Molecular Pathology: immunohistochemis… [learn more]