Overview
LCMB Microscopy Core offers live cell imaging technologies as well as standard confocal systems for immunofluorescence. Our confocal instruments are a Leica SP8 LSCM and a spinning disk confocal microscope. A Total Internal Reflection Fluorescence (TIRF) microscope is also available and Single Molecule Localization Microscopy can be performed on this system.
Contact Details
Manager: Sandeep Pallikkuth, Ph.D.
Phone: 240.760.6360
Email: sandeep.pallikkuth@nih.gov
Address: Building 37, Room 2033B, Bethesda
Established Technologies
- Live cell imaging
- Equipment includes a Leica SP8 laser scanning confocal microscope with Falcon-FLIM, a Nikon spinning disk confocal microscope, and a Nikon epifluorescence/TIRF microscope. We also have 3 imaging data processing stations.
Developing Technologies
- We are developing protocols for madSTORM for use on our TIRF instrument.
User Guidelines
Facility staff provide training on all the instruments for members of LCMB. We also provide training for outside users who need to use the spinning disk confocal microscope or the Nikon TIRF instrument. We do not provide any training for any Zeiss laser scanning confocal microscope, although we do allow experienced outside users access to our instruments. Only LCMB members can use the instruments at times when the Facility staff are not present (i.e., evenings and weekends).
Keywords
CO2FRAPFRETLeica SP8 laser scanning confocal microscopeNikon epifluorescence/TIRF microscopePALMTIRFTotal Internal Reflection FluorescenceconfocalImaging and Microscopyhumidityimmunofluorescencelocalization microscopymulti-labellingnci-coreoptical microscopyphotoactivated localization microscopyphotoactivationspinning disk confocal microscopetilingtime lapse with heattotal internal reflection fluorescenceFLIM
Contact Information
Name:
Sandeep Pallikkuth
Title:
Core Head
Address:
9000 Rockville PikeBethesda, MD 20892
Phone:
E-Mail: