Electron Microscopy Laboratory

The EML offers investigators access to unique expertise and EM technologies that allow CCR Investigators to explore new avenues of research in order to enhance the knowledge of biological systems. To assist our customers, we provide complete specimen preparation service which includes shipment of fixative, processing, embedding, sectioning, and imaging of samples. If desired, investigators can also discuss the design of custom protocols tailored to their specific needs. EML is operated by Leidos Biomedial Research Inc. on behalf of NCI as part of the Frederick National Laboratory.

Core Capabilities
The EML provides Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM) services as well as expertise in the following specialized areas: Immunoelectron microscopy, cryo-electron microscopy, negative stain analysis, ultrastructure analysis and virus particle characterization.
Instrumentation
The EM Lab suite includes four transmission electron microscopes and one scanning electron microscope. Supporting equipment includes an FEI Vitrobot, four ultramicrotomes, a high-resolution sputter coater and light microscopes with digital capture capability.

Established Technologies

• transmission electron microscopy (TEM)

• scanning electron microscopy (SEM)

• cryo-electron microscopy

• electron tomography

• nanoparticle analysis (soft matter and hard matter)

• elemental analysis by energy dispersive x-ray spectroscopy

• selected area electron diffraction

• consultation/training

• image data storage and access