Contact Information

Primary Contact

Tatiana Karpova


41 Medlars Drive
Bldg 41/ C615
Bethesda, MD 20892


The core provides access to several different state-of-the-art 3D microscopes as well as computers to visualize and process image data. The facility houses equipment for 2D or 3D imaging of fixed and living specimens. High resolution images can be obtained by confocal microscopy or deconvolution, and super-resolution techniques SIM, STORM, MINFLUX. Single Molecule tracking is supported on MINFLUX, and custom HILO-illumination microscopes.

Established Technologies

Facility personnel are available for consultation about the design of imaging experiments and/or the analysis of image data. Special expertise is available for assistance with live cell imaging as well as deconvolution microscopy. Facility personnel are also experienced in the other techniques listed below and can provide expertise and software for quantitative analysis of images.

  • 2D or 3D imaging of fixed and living specimens
  • High-resolution imaging using confocal microscopy or deconvolution
  • Protein colocalization
  • Fluorescence photobleaching techniques, such as FRAP
  • Analysis of protein interactions by FRET
  • Super Resolution (SIM; STORM, MINFLUX)
  • Single Molecule Tracking by HILO-illumination and MINFLUX.

Major Instrumentation

  • Delta Vision Elite wide-field deconvolution microscope
  • NIKON  wide-field microscope
  • ELYRA Super Resolution Microscope
  • Carl Zeiss LSM780 Microscope
  • Carl Zeiss LSM880 Microscope
  • Custom HILO-illuminated microscopes
  • MINFLUX nanoresolution/nanotracking microscope
  • Lattice Light Sheet (3i) microscope

User Guidelines

Open to all NCI intramural researchers. Researchers from other NIH institutes are also welcome to contact us. The Core collaborates on Single Molecule Tracking, Nano resolution projects and projects requiring extensive support of core staff and supports and trains users for conventional techniques/microscopes.

New Users interested in using the core’s microscopes must first contact Dr. Karpova. Following the initial request, Dr. Karpova will meet with the user and his/her principal investigator to discuss the research project. Advice will be provided to the user regarding the advantages/limitations of the different forms of microscopy available, both in this core and elsewhere. The staff will also help the user develop an overall research strategy, design experiments, and solve specific experimental problems.

Consultation is also available regarding image analysis and interpretation and/or selection and purchase of microscopy hardware and image processing software.


Delta Vision Core deconvolution microscopeELYRA Super Resolution MicroscopeFCSFRAPFRETPALMPhotoactivated Localization MicroscopySIMSTORMStructural Illumination MicroscopyZeiss LSM780confocal microscopydeconvolutionImaging and Microscopyfluorescencehigh resolution imagingimaginglive-cell imagingnci-coreoptical sectioningprotein colocalizationNanoresolutionSingle Molecule TrackingMINFLUXLattice Light SheetZeiss LSM 880Custom HILO-illumination microscope