Overview
The FNLCR Molecular Histopathology Laboratory (MHL) provides comprehensive veterinary pathology support for animal health monitoring, biomarker discovery and validation, drug development, genomics, and proteomics on a cost recovered basis. The MHL is organized into multiple process groups with cross-trained experts in animal study design, phenotyping of genetically engineered mice (GEM), immunopathology, anatomic pathology, toxicological pathology, and specimen classification/selection for tissue microarray (TMA) construction or laser capture microdissection (LCM).
Established Technologies
- Anatomic Pathology
- Toxicologic Pathology
- Mouse Embryology
- Genetically Engineered Mouse Phenotyping
- Hematology and Clinical Chemistry (non-human)
- Tissue Embedding/Block Preparation (paraffin, OCT, Resin)
- Tissue Microarray Production
- Microtomy (paraffin, frozen, vibratome). Serial and step sectioning.
- Histological Stain
- Molecular Pathology: immunohistochemistry (IHC); in situ hybridization (ISH)
- Nucleic Acid Isolation from fresh/frozen/fixed tissues, paraffin blocks, cut slides, paraffin cores/ribbons
- Laser Capture Microdissection (LCM) and Laser Cutting (LC)
- Digital Image Capture (Whole slide Bright field / Fluorescent)
- Digital Image Analysis via Indica Labs HALO
User Guidelines
To request services from this core facility, submit a requisition through the NCI at Frederick Accessioning System NAS. The lab services the NIH communities in Bethesda and Frederick, Maryland.
Keywords
Anatomic PathologyAnimal Necropsy Support including Tissue HarvestBeta GalCryotomy frozenDigital Image Analysis (Bright field)Digital Image Capture (Whole slide Bright field/Fluorescent)Enzyme StainsGenetically Engineered Mouse (GEM) PhenotypingHistological StainsLaser Capture Microdissection (LCM)Laser Cutting (LC)Microtomy paraffinMolecular PathologyMouse EmbryologyMultiplex Tissue ImagingNucleic Acid IsolationOCTPHLRodent Clinical ChemistryRodent HematologySerial sectioningTissue Embedding/Block Preparation (paraffinTissue Microarray ProductionToxicologic Pathologycut slidesAnimal ResourcesImaging and Microscopyfile sharingfixed tissuesfreshfrozenimmunohistochemistry (IHC)in situ hybridization (ISH)nci-coreparaffin blocksparaffin coresparaffin ribbonsplastic)step sectioningvibratomeRNAscope
Contact Information
Name:
Dr. Larry Sternberg
Title:
Lab Director
Address:
Bld 469, Rm 218Miller Drive
Frederick, MD 21702
Frederick, MD 21702
Phone:
E-Mail:
Additional Core Staff
Name:
Stephanie Smith
Title:
Administrative Coordinator
Phone:
E-Mail: