Overview

The FNLCR Molecular Histopathology Laboratory (MHL) provides comprehensive veterinary pathology support for animal health monitoring, biomarker discovery and validation, drug development, genomics, and proteomics on a cost recovered basis. The MHL is organized into multiple process groups with cross-trained experts in animal study design, phenotyping of genetically engineered mice (GEM), immunopathology, anatomic pathology, toxicological pathology, and specimen classification/selection for tissue microarray (TMA) construction or laser capture microdissection (LCM).

Established Technologies

  • Anatomic Pathology
  • Toxicologic Pathology
  • Mouse Embryology
  • Genetically Engineered Mouse Phenotyping
  • Hematology and Clinical Chemistry (non-human)
  • Tissue Embedding/Block Preparation (paraffin, OCT, Resin)
  • Tissue Microarray Production
  • Microtomy (paraffin, frozen, vibratome). Serial and step sectioning.
  • Histological Stain
  • Molecular Pathology: immunohistochemistry (IHC); in situ hybridization (ISH)
  • Nucleic Acid Isolation from fresh/frozen/fixed tissues, paraffin blocks, cut slides, paraffin cores/ribbons
  • Laser Capture Microdissection (LCM) and Laser Cutting (LC)
  • Digital Image Capture (Whole slide Bright field / Fluorescent)
  • Digital Image Analysis via Indica Labs HALO

User Guidelines

To request services from this core facility, submit a requisition through the NCI at Frederick Accessioning System NAS. The lab services the NIH communities in Bethesda and Frederick, Maryland.

Contact Information
Name:
Dr. Larry Sternberg
Title:
Lab Director
Address:
Bld 469, Rm 218
Miller Drive
Frederick, MD 21702
Additional Core Staff
Name:
Stephanie Smith
Title:
Administrative Coordinator