The FNLCR Molecular Histopathology Laboratory (MHL) provides comprehensive veterinary pathology support for animal health monitoring, biomarker discovery and validation, drug development, genomics, and proteomics on a cost recovered basis. The MHL is organized into multiple process groups with cross-trained experts in animal study design, phenotyping of genetically engineered mice (GEM), immunopathology, anatomic pathology, toxicological pathology, and specimen classification/selection for tissue microarray (TMA) construction or laser capture microdissection (LCM).
- Anatomic Pathology
- Toxicologic Pathology
- Mouse Embryology
- Genetically Engineered Mouse Phenotyping
- Hematology and Clinical Chemistry (non-human)
- Tissue Embedding/Block Preparation (paraffin, OCT, Resin)
- Tissue Microarray Production
- Microtomy (paraffin, frozen, vibratome). Serial and step sectioning.
- Histological Stain
- Molecular Pathology: immunohistochemistry (IHC); in situ hybridization (ISH)
- Nucleic Acid Isolation from fresh/frozen/fixed tissues, paraffin blocks, cut slides, paraffin cores/ribbons
- Laser Capture Microdissection (LCM) and Laser Cutting (LC)
- Digital Image Capture (Whole slide Bright field / Fluorescent)
- Digital Image Analysis via Indica Labs HALO
To request services from this core facility, submit a requisition through the NCI at Frederick Accessioning System NAS. The lab services the NIH communities in Bethesda and Frederick, Maryland.