NATIONAL CANCER INSTITUTE - CANCER.GOV

Contact Information

Primary Contact

Jeff Carrell
Manager, CCR Frederick Flow Core

Location

Building 560, Room 31-54
Frederick, MD 21702

Overview

The CCR-Frederick Flow Cytometry Core provides research support and cytometry instruments for the Frederick-CCR community, including analysis and sorting services, instrument maintenance, new user training, and technical consultation.  The lab is supported by the Cancer Innovation Lab of CCR.

Typical Flow Cytometry Applications
  • Immunophenotyping, multicolor identification of immune cell subsets
  • Apoptosis assays, including Annexin V, TUNEL, caspase activation, or mitochondrial membrane potential
  • Cell cycle (DNA content) analysis
  • Cell health and proliferation monitoring
  • Sterile Cell sorting (physical separation) for bulk collection or deposition in 96- or 384-well plates
  • Sorting and analysis of cells by fluorescent reporters such as GFP, BFP, mCherry, etc.

Major Instrumentation

  • BD FACS Symphony S6 cell sorter in BSL2+ lab with 5 lasers (blue/488nm, red/640nm, violet/405nm, yellow-green/561nm, UV/350nm).
  • BD FACSAria II SORP cell sorter in BSL2+ lab with 4 lasers (blue/488nm, red/640nm, violet/405nm, yellow-green/561nm).
  • BD FACSAria II cell sorter with 5 lasers (blue/488nm, red/640nm, violet/405nm, yellow-green/561nm, UV/350nm).
  • BD FACSymphony A5 analyzer with 5 lasers (blue/488nm, red/640nm, violet/405nm, yellow-green/561nm, UV/350nm).
  • BD LSRII SORP analyzer with 4 lasers (blue/488nm, red/640nm, violet/405nm, yellow-green/561nm).
  • BD LSRII Fortessa analyzer with 4 lasers (blue/488nm, red/640nm, violet/405nm, yellow-green/561nm).
  • Miltenyi Biotec MACSQuant-16 analyzer with 3 lasers (blue/488nm, red/640nm, violet/405nm) and optional 96-well sampling.
  • Cytek Aurora Spectral Cytometer with 5 lasers (blue/488nm, red/640nm, violet/405nm, yellow-green/561nm, UV/350nm).

User Guidelines

The CCR-Frederick Flow Core instruments and services are available to IRP investigators in Frederick. To use core resources (instruments, analysis or cell sorting services, or technical consultation) please contact Jeff Carrell directly at jeff.carrell@nih.gov. 

Trained users from CCR may use the analysis cytometers without usage fees; staff operates cell sorter instruments with an hourly fee to recover some costs. Investigators outside CCR may use the core on a case-by-case basis. For the fee structure, please contact Jeff Carrell at jeff.carrell@nih.gov.

The core has BSL2 and BSL2+ labs for cell sorting. When requesting cell sorting, please provide as much detail as possible including cell species, fluorescent labels, number of samples, cell counts, gating strategy and project ID. Scheduling is most flexible 1-2 weeks ahead of time, but we try to accommodate urgent requests when possible.

Publications

  • Carrell J, Groves CJ. OMIP-043: Identification of human antibody secreting cell subsets. Cytometry A. 2018 Feb;93(2):190-193. doi: 10.1002/cyto.a.23305. Epub 2017 Dec 29. PMID: 29286577
  • Groves CJ, Carrell J, Grady R, Rajan B, Morehouse CA, Halpin R, Wang J, Wu J, Shrestha Y, Rayanki R, Kolbeck R, Wang Y, Herbst R. CD19-positive antibody-secreting cells provide immune memory. Blood Adv. 2018 Nov 27;2(22):3163-3176. doi: 10.1182/bloodadvances.2017015172. PMID: 30478153; PMCID: PMC6258909
  • Britton ZT, London TB, Carrell J, Dosanjh B, Wilkinson T, Bowen MA, Wu H, Dall'Acqua WF, Marelli M, Mazor Y. Tag-on-Demand: exploiting amber codon suppression technology for the enrichment of high-expressing membrane protein cell lines. Protein Eng Des Sel. 2018 Oct 1;31(10):389-398. doi: 10.1093/protein/gzy032. PMID: 30753634
  • Wright PW, Li H, Rahman MA, Anderson EM, Karwan M, Carrell J, Anderson SK. The KIR2DL1 intermediate upstream element participates in gene activation. Immunogenetics. 2023 Dec;75(6):495-506. doi: 10.1007/s00251-023-01321-9. Epub 2023 Oct 6. PMID: 37801092; PMCID: PMC10651540
  • Bafor EE, Martin T, Carrell J, Karwan M, Kimmel AE, Omogiade UG, Sanford M, Young HA, Valencia JC. Isolation of single cells from individual mouse ovaries for flow cytometry and functional analysis. STAR Protoc. 2023 Dec 15;4(4):102710. doi: 10.1016/j.xpro.2023.102710. Epub 2023 Nov 14. PMID: 37963022; PMCID: PMC10679862

Keywords

AriaBDBiosciencesCytek AuroraFACSFlowJoFortessaLSRIISymphonyapoptosiscell cyclecell sortingflow cytometryimmunophenotypingnci-corespectral cytometryFlow CoretroubleshootingCILCancer Innovation Laboratory