Flow Cytometry

Flow Cytometry Core (LGI) offers established technologies to support studies using flow cytometry and cell sorting. Instrumentation: 2 FACSCaliburs – 2 lasers (488, 635), LSRII – 5 lasers (355, 405, 488, 561, 635), LSR Fortessa – 4 lasers (355, 405, 488, 639), FACS Aria (standard) – 3 lasers (405, 488, 639), FACS Aria (SORP) – 3 lasers (355, 488, 639), MoFlo Astrios – 5 lasers (355, 405, 488, 561, 640), Established Technologies: Applications that run on FACS Caliburs include: Immunophenotyping (up to 4-color), … [learn more]

The NCI Vaccine Branch Flow Cytometry Core is a full-service facility within the Center for Cancer Research that supports over 150 users representing 26 laboratories. The Core Facility provides instrument and software training, technical expertise, assay development, and experimental design for all users. The Core also offers sample preparation protocols and reagent recommendations, including fluorochrome panel setups for each flow cytometer and reagent cross-reactivity among species. The Core Manager provides assistance with data analysis and figure preparation for flow cytometry data present… [learn more]

The CCR-Frederick Flow Cytometry Core Facility provides research support to the Frederick-CCR community, including cytometry analysis and sorting services, instrument maintenance, new user training, and technical consultation. Typical Assays Performed by Core Instruments: Immunophenotyping of mouse and human cells, Apoptosis assays, including Annexin V, TUNEL, caspase activation, or mitochondrial membrane potential, Cell cycle (DNA content) analysis, Cell health and proliferation monitoring, Sterile Cell sorting (physical separation) for bulk collection or deposition … [learn more]

Clinical Support Laboratory – Flow Cytometry Section is a laboratory specializing in providing immunophenotyping support of NCI intramural clinical trials, though assessments may also be performed using cells from Non-human primates and other species. The CSL Flow cytometry core is operated by Leidos Biomedical Research Inc. on behalf of NCI as part of the Frederick National Laboratory. Established Technologies: Immunophenotyping (up to 15-color), Specialized immunophenotyping for clinical trial research – ICS and tetramer staining also available.  Frequently customized pan… [learn more]