Flow Cytometry

The CCR Building 41 Flow Cytometry Core is a full-service facility within the Center for Cancer Research that supports over 150 users representing 26 laboratories. The Core Facility provides instrument and software training, technical expertise, assay development, and experimental design for all users. The Core also offers sample preparation protocols and reagent recommendations, including fluorochrome panel setups for each flow cytometer and reagent cross-reactivity among species. The Core Manager provides assistance with data analysis and figure preparation for flow cytometry data presentat… [learn more]

The Flow Cytometry Core (LGI) offers established technologies to support studies using flow cytometry and cell sorting. Established Technologies Applications that run on FACS Caliburs include: Immunophenotyping (up to 4-color), Intracellular markers, including cytokines and phosphoproteins, Cell cycle analysis using propidium iodide for mammalian cells and cytox green for yeast, Cell cycle-associated BRDU labeling of S-phase cells and phosphohistone H3 labeling of mitotic cells, Apoptosis by annexin V binding, TUNEL, caspase, or FLICA, Detection o… [learn more]

The CCR-Frederick Flow Cytometry Core provides research support and cytometry instruments for the Frederick-CCR community, including analysis and sorting services, instrument maintenance, new user training, and technical consultation.  The lab is supported by the Cancer Innovation Lab of CCR. Typical Flow Cytometry Applications Immunophenotyping, multicolor identification of immune cell subsets, Apoptosis assays, including Annexin V, TUNEL, caspase activation, or mitochondrial membrane potential, Cell cycle (DNA content) analysis, Cell health and proliferation monitoring, … [learn more]