The NCI Vaccine Branch Flow Cytometry Core is a full-service facility within the Center for Cancer Research that supports over 150 users representing 26 laboratories. The Core Facility provides instrument and software training, technical expertise, assay development, and experimental design for all users. The Core also offers sample preparation protocols and reagent recommendations, including fluorochrome panel setups for each flow cytometer and reagent cross-reactivity among species. The Core Manager provides assistance with data analysis and figure preparation for flow cytometry data presentation in publications. While the core staff are experienced in all areas of flow cytometry, they also have extensive expertise in 20+ color flow cytometry, non-human primate flow cytometry, mouse flow cytometry, and immune monitoring by flow cytometry.
Established Technologies
- Cell sorting of both infectious (HIV, SIV, HTLV) and non-infectious samples
- Multi-color immunophenotyping
- Cell cycle analysis
- Proliferation analysis (Ki67, PCNA, BrdU, CFSE)
- Apoptosis measurement (Annexin V, JC1, Active Caspase 3, TUNEL)
- Intracellular cytokine analysis
- Stem cell analysis and sorting
- Calcium flux analysis
- Bead array analysis of soluble proteins
- Phosphorylated protein analysis
- Fluorescent protein analysis and sorting
- Quantitative flow cytometry
- Spectral flow cytometry
- AutoMACS Pro magnetic bead separator