EIB Microscopy Facility has the following instruments:
As a multi-user facility, the different instruments provide a wide range of imaging modes for EIB scientists, from standard immunohistochemistry, through brightfield and wide-field epifluorescence imaging, to highly complex live cell confocal microscopy and super-resolution STED imaging. LSM 880 microscope is capable of advanced imaging techniques, such as FRAP, FRET, photoactivation/photoswitching, laser microirradiation, and spectral imaging.
We continually develop or refine microscopy-based assays, aimed primarily at addressing questions related to cellular immunology, but we are not engaged in developing new microscope technology.
We also advise users regarding image processing and analysis, and, when needed, write custom macros for ImageJ to facilitate those tasks.
Dr. Wisniewski provides individual training for all new users and assists in both the use of Facility equipment and in the interpretation and analysis of data. EIB researchers have priority use of the facility, but it is open to the NCI/NIH research community as a whole.
Follow-up training sessions are conducted until users are comfortable operating the equipment. In general, users are encouraged to operate the equipment themselves. Users of the facility are requested to notify the Facility Head when images collected in the facility are published, or used in presentations at meetings, site visits, etc.