Imaging and Microscopy

OMAL focuses its research and development activities to quantitatively understand the molecular basis of three-dimensional (3D) cell organization, motility, invasion, and differentiation using fixed samples and live, 3D tissue culture models (i.e., translational models). OMAL is a CCR-dedicated facility. Prospective users and collaborators should first consult the following website before contacting OMAL staff: https://confocal.ccr.cancer.gov/nci-microscopy-core-labs/frederick/omal/.OMAL is operated by Leidos Biomedial Research Inc. on behalf of NCI as part of the Frederick National Labo… [learn more]

The Spatial Imaging Technology Resource (formerly the Nanoscale Protein Analysis Section of the Collaborative Protein Technology Resource or CPTR) provides expertise and service in state-of-the-art protein analysis technologies to advance CCR research in basic discovery and translation/clinical studies. Applications include: Quantitative profiling of cell signaling and post-translational modification, Measurement of cytokine, metabolite and serum/plasma markers , Identification and validation of biomarkers & therapeutic targets, Assessment of on‐ and off‐target drug activity and … [learn more]

CCR VOLUME Electron Microscopy (CVEM) aims to develop new techniques in volume electron microscopy (vEM) and, in collaboration with CCR Investigators, apply these techniques to research cell biological questions well-suited for study by high-resolution 3D electron microscopy. CVEM, headed by Kedar Narayan, uses focused ion beam scanning electron microscopy (FIB-SEM), array tomography (AT), electron tomography (ET) and other complementary methods to visualize ultrastructural features of cells and tissues, including cell membranes, cellular organelles, and virus particles. CVEM is a CCR-dedicate… [learn more]

EIB Microscopy Facility has following instruments: 1) Abberior STEDYCON super-resolution (STED) microscope; 2) Zeiss LSM 880-NLO confocal / two-photon system; 3) Yokogawa CSU-W1 spinning disck confocal microscope and 4) Zeiss AxioObserver Z1 wide field microscope. As a multi-user facility, the different instruments provide a wide range of imaging modes for EIB scientists, from standard immunohistochemistry, through brightfield and wide-field epifluorescence imaging, to highly complex live cell confocal microscopy and super-resolution STED imaging. LSM 880 microscope is capable of advanced … [learn more]

LCBG Microscopy Core Facility offers imaging technologies and training. The facility has established instrumentation for for 2D and 3D imaging of both fixed and living specimens. Equipment include: Zeiss AxioObserver.Z1 epifluorescent microscope, Zeiss AxioScan.Z1 slide scanner, Zeiss LSM780 confocal microscope, and Nikon Eclipse Ti2 widefield microscope. [learn more]

The goal of Artificial Intelligence Resource (AIR) is to make AI tools available to Clinical Cancer Research (CCR) investigators. The strength of AI is that algorithms can be trained to seek specific information that may scientifically or clinically important. AIR will mainly focus on “Computer Vision” which analyzes medical images, such as radiologic, digital pathology, video/endoscopy and optical imaging among others. Examples of potential projects include developing better screening, detection methods or predictive markers, or improving procedures among many others. Both clinical … [learn more]

The NCI High-Throughput Imaging Facility (HiTIF) works in a collaborative fashion with NCI/NIH Investigators by providing them with the necessary expertise, instrumentation, and software to develop and execute advanced High-Throughput Imaging (HTI) assays. These can be paired to screen libraries of RNAi or CRISPR/Cas9 reagents to discover and characterize novel cellular pathways by functional genomics. In addition to functional genomics screens, HiTIF also develops HTI assays to extract and quantify single-cell information about the molecular mechanisms underlying rare or heterogeneous b… [learn more]

The CCR Microscopy Core provides NCI investigators access to state-of-the-art imaging tools and techniques, including light sheet fluorescence, high-resolution confocal, multi-photon, and super-resolution microscopy. The mission of the CCR Microscopy Core Facility is to support the microscopy and digital imaging needs of investigators studying the biological structures and cellular processes involved in the cell biology of cancer by applying our diverse imaging resources, expertise, and quality customer service. The goal is to provide cutting-edge imaging technology, expert consultation … [learn more]