Clinical Support Laboratory – Cellular Immunology / Cell Culture offers a spectrum of bioassays to assess cellular responses to antigens and mitogens (flow cytometry, ELISpot/Fluorospot, cytotoxicity assays, proliferation assays). This section also offers Olink assay (Target panels and Reveal). This section also generates fibroblasts from skin biopsies, EBV-transformed cell lines, and expands existing cell lines.
Clinical Support Laboratory– Lymphokine Testing Section runs and analyzes research samples (e.g. human, mouse, NHP, rat) for biomarkers in serum, plasma, cell culture supernatant and cell lysates. Utilizes Meso Scale Discovery and Luminex platforms, as well as conventional ELISA. This section of the Clinical Support Laboratory offers a CLIA assay to test IFN-gamma, IL-1 beta, IL-6 and TNF-alpha.
Clinical Support Laboratory– Clinical Trials Processing supports human clinical trials by preparing collected biological samples into various usable products. After samples are received, they are assigned unique identification numbers and processed to generate products such as PBMCs, plasma, serum, and cell pellets. After samples are cryopreserved, they can be shipped to the NCI-Frederick Central Repository. This section also provides DNA and RNA extraction services from various sources.
Established Technologies
• OLINK (Target panels, Reveal)
• Flow cytometry (Canto II and Fortessa)
• Elispot Assays
• Dual or Triple Color Fluorospot Assays
• Murine Immunoassays
• Cytotoxic T cell assays (Chromium Release Assays)
• Antigen and mitogen-induced cell proliferation
• Mixed Lymphocyte Reactions
• Antibody-Dependent Cell-Mediated Cytotoxicity
• In vitro Stimulation/Cytokine Induction
• Meso Scale Discovery (MSD)
• Luminex
• Conventional ELISA
• CLIA (IFN-g, IL-1b, IL-6, TNF-a)
• DNA, RNA extraction
• Conventional ELISA
• Processing and aliquoting of human and canine clinical blood samples.
• Process samples are logged in a database, and samples can be shipped to the NCI-Frederick Central Repository.
MSD plate reader, BD Canto II and Fortessa for flow cytometry, Olink, ImmunoSpot Analyzer, CryoMed Controlled-Rate Freezers
Currently, CCR, NIAID and DCEG receive support from the Clinical Support Laboratory. Project scope is defined in consultation with the customer and cost estimates based on actual time and materials are provided for investigator review/approval before any project is initiated. Support can be for research or enter at the early stages of clinical trial development to aid in developing a comprehensive strategy for immunological assays. To request services from this NCI core facility, you must submit your requisition through NAS. Requests from other IC investigators are considered on a case-by-case basis.