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CLIA Molecular Diagnostics Laboratory
Frederick, Maryland
Services Offered
Mutation Detection:For PCR and Sanger sequencing both testing and bioinformatics interpretation are conducted. This method is currently being used in patients with chronic granulomatous disease (CGD), WHIM syndrome, and other autoimmune disorders. Because Sanger is a “general method,” it can also be and has been used for other mutation detection, including assays for inherited diseases and cancer-related genes, as well as verification of next-generation sequencing discoveries. Assays can be optimized for any new gene(s) of interest. We perform traditional polymerase chain reaction a… [learn more]
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Clinical Support Laboratory
Frederick, MD
Services Offered
The Clinical Support Laboratory offers processing, tracking, and testing of a broad range of clinical samples. Support can begin at the early stages of clinical trial development to aid in developing a comprehensive strategy for sample collection, processing, and testing with a special emphasis on immunologic testing. The lab collaborates with partners to develop customized assays or transfer existing assay technology with assay validation, data analysis and interpretation of results. Processing specializes in PBMC isolation and controlled rate freezing, ELISA based biomarker testing, and mult… [learn more]
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Laboratory of Cell Mediated Immunity
Frederick, MD
Services Offered
Laboratory of Cell Mediated Immunity offers a spectrum of bioassays to assess cellular responses to antigens and mitogens including ELISPOT, cytotoxicity assays and proliferation assays.Established TechnologiesElispot AssaysDual or Triple Color Fluorospot Assays Murine ImmunoassaysCytotoxic T cell assays (Chromium Release Assays) Antigen and mitogen-induced cell proliferation Mixed Lymphocyte Reactions Antibody-Dependent Cell-Mediated Cytotoxicity Cell Metabolic Analysis via Seahorse In vitro Stimulation/Cytokine Induction [learn more]
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NCI Blood Processing Core
Bethesda, MD
Services Offered
The Blood Processing Core monitors viral load in patients with HIV and performs sequential studies using samples obtained from patients with cancer, AIDS, chronic granulomatous disease, or other diseases associated with immunologic dysfunction. The core collects clinical specimens from inpatient units and clinics within the National Institutes of Health Clinical Center. We then oversee the processing and cryopreservation of human serum, plasma, peripheral blood mononuclear cells, and other specimens. Our technicians are also trained and qualified to execute preclinical animal protocols and ro… [learn more]