NATIONAL CANCER INSTITUTE - CANCER.GOV

Contact Information


Primary Contact

Asokan Anbanandam, Ph.D.
Senior Scientist

Location

Building 538, Room 187
Frederick, Md 21702

Overview

The NMR Facility for Biological Research operates six NMR spectrometers with proton resonance frequencies from 850 to 500 MHz. Three of the highest field spectrometers (850, 700, 600) are equipped with higher sensitivity cryoprobes optimized for high-resolution multi-dimensional and relaxation NMR experiments applicable to small to large biological systems studied in solution. These high field instruments are dedicated to research projects conducted by laboratories within Center for Structural Biology.  The second 600 MHz spectrometer is set up as reconfigurable development system with multiple probes allowing for high-resolution liquid state as well as biosolids Magic Angle Spinning (MAS) experiments and is sometimes used to perform experiments with special purpose probes.

The Facility operates a fully automated 500 MHz spectrometer designated as an “open access” instrument to all the FNLCR community. This spectrometer is equipped with broadband X-H/F type nitrogen cooled cryoprobe which is optimized for work on small molecule samples and serves as an analytical tool for many users – particularly from the Chemical Biology Laboratory.

Major Instrumentation

Bruker 850
Bruker 700
Bruker 600
Bruker 600 (with Bio-Solids capability)
Bruker 500

User Guidelines

The high field spectrometers (850, 700, 600) are dedicated to research projects conducted by laboratories within Center for Structural Biology. The fully automated 500 MHz spectrometer designated as an “open access” instrument available to all of the Frederick National Laboratory for Cancer Research (FNLCR) community.

Publications

  • Buel, G. R., Chen, X., Myint, W., Kayode, O., Folimonova, V., Cruz, A., Skorupka, K. A., Matsuo, H., Walters, K. J. E6AP AZUL interaction with UBQLN1/2 in cells, condensates, and an AlphaFold-NMR integrated structure. Structure 31(4), 395-410 (2023). doi: https://doi.org/10.1016/j.str.2023.01.012
  • Buel, G. R., Chen, X., Kayode, O., Cruz, A., and Walters, K. J. 1H, 15N, 13C backbone and Cb resonance assignments for UBQLN1 UBA and UBAA domains. Biomol. NMR assign. (2023) https://doi.org/10.1007/s12104-023-10127-5.

Keywords

Biological NMR, Structural BiologyDrug Discovery by NMR