Bruker AVANCE 400 and 500 MHz NMR instruments. Helium Cryoprobe technology on the 500 MHz machine for added sensitivity, especially for Carbon-13 spectra. Access to a second 500 MHz instrument with Prodigy  Liquid Nitrogen-cooled cryoprobe . User Accounts can be created for automated setup and experiment acquisition.  Use of ICONNMR automated software with 24-slot sample changers on each machine. User training available. Some service to those who are unfamiliar with NMR can be performed either as a one-time aid for compound identification or as a collaborative project for more extensive … [learn more]

FOR ALL NIH PROJECTS PLEASE CONTACT DINA DIRECTLY VIA EMAIL dina.sigano@nih.gov **** The Chemical Synthesis Group is a component of the Chemical Biology Laboratory. This facility provides synthetic chemistry resources and expertise to the NCI Intramural community. Contact Details Head: Dr. Dina Sigano Phone: 301-846-5995 Email: siganod@mail.nih.gov Address: 376 Boyles Street, Frederick, MD 21702 The facility’s capabilities include: Providing expertise, consultation, and experience in areas of chemical synthesis and purification. Providing … [learn more]

The CCR Molecular Modeling Core, under the direction of Dr. Stewart Durell, was established to provide a convenient way for experimentalists to take advantage of molecular modeling and computational biology. In the framework of consultations, scientists can explore how such tools could benefit their projects. The Core then acts as a clearinghouse to help set up and use appropriate software, and/or form collaborations with other scientists. Upon mutual agreement, Core personnel will enter into a collaboration to do the modeling work themselves. All services of the Core are free … [learn more]

Small-angle X-ray scattering (SAXS) is a powerful technique in structural biology. SAXS enables the investigation of the structure and dynamics of biomacromolecules under various buffer conditions, including salt concentration, pH, presence or absence of ligands, and changes in temperature and pressure. SAXS provides insights into global information about the size and shape of biomacromolecules, conformational changes, complex formation, quaternary structure, oligomerization behavior, assembly and folding, flexibility of fragments, or entire structures, etc. SAXS data can also be used in t… [learn more]

The research conducted within the Synthetic Biologics Core (SBC) Facility has a dual role:  1) generate chemical biology tools and drug candidates for molecular targets identified by NCI research groups and 2) develop novel effective methods and tools for more effective molecular discovery. Chemical biology tools for studying protein functions have many advantages over applications of genetic approaches for studying protein functions. However, wide use of chemical probes is hampered by the difficulties in their generation and characterization. Because the majority of cancer driver… [learn more]