The Biophysics Core’s mission is to provide support in the study of macromolecular interactions, dynamics, and stability by offering consultations, training, professional collaborations, and instrument access.
General Services
- Multi-technique molecular interaction studies
- Kinetic and thermodynamic characterization of interactions
- Biophysical characterization of protein complexes and molecular assemblies
- Biophysical characterization of integral membrane proteins
- Characterization of exosomes, viruses and nanoparticles
- Sample quality control and optimization
- Stability and interaction screening
Established Technologies
- Mass Photometry (MP)
Mass measurements of single molecules in solution with 2% mass accuracy
- Bio-Layer Interferometry (BLI: Octet RED96), Surface Plasmon Resonance (SPR)
Real time monitoring of binding events to determine on-rates, off-rates, and binding affinity
- Microscale Thermophoresis (MST: Monolith NT.115)
Measurements of challenging biomolecular interactions in solution
- Differential Scanning Fluorimetry (nano-DSF: Tycho)
Fast and comprehensive protein quality determination: purity, functionality, folding, similarity, and concentration
- Nanoparticle Tracking Analysis (NTA: ZetaView TWIN)
Measurements of size, concentration and zeta potential of exosomes, microvesicles, viruses, and nanoparticles
- Multi Angle Light Scattering, Field Flow Fractionation (SEC-FFF-MALS: Eclipse AF4, DAWN Helos II)
Molecular separations and measurements of molecular mass, size, and shape of macromolecules in solution
- Dynamic Light Scattering (DLS: Dynapro Nanostar)
Fast molecular size measurements of proteins, micelles, liposomes, and nanoparticles
- Circular Dichroism Spectropolarimeter (CD: Applied Photophysics Q100)
Determination of the secondary structure of proteins and nucleic acids in solution
- Analytical Ultracentrifugation (AUC: Optima XLI)
Studies of oligomerization, heterogeneity, and biomolecular interactions in solution
- Isothermal Titration Calorimetry (ITC: iTC200)
Label-and immobilization-free studies of biomolecular interactions in solution
- Differential Scanning Calorimetry (DSC: VP-DSC)
Precise analysis of thermally-induced structural transitions in proteins, DNA, RNA, micellar complexes, and other biomolecular systems
- Fluorometry
Label-and immobilization-free studies of biomolecular interactions in solution