Illumina Proposal and Sample Submission Questions
What services does the Sequencing Facility provide?
Who can order services through the Sequencing Facility?
How do I submit a sequencing proposal?
How do I submit samples?
What are the quality/quantity requirements for submitted samples?
What happens after my sample is submitted?
How do I check the status of my order/sample?
Answers
What services does the Sequencing Facility provide?
Please see the services page for a detailed list of projects we support. If your project design is not listed, please contact ccrsfhelp@mail.nih.gov, or the Sequencing Facility Director, to discuss the feasibility of a custom project.
Who can order services through the Sequencing Facility?
All NIH research labs are eligible to order services through the Sequencing Facility.
How do I submit a sequencing proposal?
Please complete a sequencing proposal form at NAS Request. You may also contact ccrsfhelp@mail.nih.gov to discuss the available platforms and best choice for your project.
How do I submit samples?
Before submitting samples, ensure that the sequencing proposal has been approved and the NAS request submitted. Our service is listed under: NCI-CCR Sequencing Facility (/resources/sequencing-facility/).
You may then submit samples by delivering them to ccrsfhelp@mail.nih.gov, at the ATRF Room D3040 (instructions for sample delivery). Be sure to include a sample manifest with your submission.
What are the quality/quantity requirements for submitted samples?
All samples must be sent in a 1.5 ml or 2 ml tubes.
| Type of Library | Minimum DNA/RNA Requirement for Library Construction | Recommended DNA/RNA for Optimal Library Construction | Maximum Sample Volume Requirement for Library Construction | Additional Requirements |
| ChIP DNA Sequencing | 5 ng | 10 ng | 30 μL | Bulk of the DNA fragments in the 100-300 bp range |
| gDNA Sequencing | 100 ng | 1 μg | 30 μL | DNA should be as intact as possible with no contamination, OD260/280 1.8–2.0 |
| mRNA Sequencing | 100 ng | 1 μg | 50 μL | RIN should be at least 8.0, DNase treated |
| mRNA ultralow Clonetech | 100 pg | 10 ng | 10 μL | RIN should be at least 8.0, DNase treated |
| microRNA Sequencing | 100 ng | 1 μg | 6 μL | |
| Total RNA Sequencing | 100 ng | 1 μg | 10 μL | DNase treated, FFPE and degraded RNA can be used |
| Total RNA ultralow | 10 ng | 1 μg | 10 μL | DNase treated, FFPE and degraded RNA can be used |
What happens after my sample is submitted?
Before sequencing, we will perform an internal QC to confirm the information in the sample manifest and notify you if any samples do not meet minimum sequencing requirements. You will then be able to choose whether to resubmit those samples or continue and sequence them. You will be notified again when the analysis on each sample is completed and available for download.
How do I check the status of my order/sample?
You may be able to estimate the stage of your samples using our general processing flowchart. For more specific information, please contact us at ccrsfhelp@mail.nih.gov.