Bethesda, MD
Trans NIH Facility
The National Institutes of Health (NIH) Office of Research Services (ORS) Division of Radiation Safety (DRS) Radioactive Materials Control and Analysis Branch supports radioactive materials packaging/shipping. Established Technologies In accordance with Department of Transportation ( Read More...
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Bioinformatics
In addition to the Heatmap and the FeaturePlot shown previously, two other options easily accessible through Seurat are the Dot Plot and Violin Plot. The dot plot can visualize relative expression level and expression fraction. Read More...
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Bioinformatics
The Seurat v5 object doesn’t require all assays have the same cells. In this case, Cells() can be used to return the cell names of the default assay while colnames() can be used to Read More...
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Bioinformatics
04/28/2021 - Register Description: This webinar offers an introduction to FlowJo, an application designed to help with data archiving, analysis, and reporting. The training begins by showing how to load samples and then outlines the steps Read More...
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Bioinformatics
Scatter plots are useful to visually inspect similarity (correlation) between variables. For example, we can see how closely two replicates from the TCGA lung adenocarcinoma data set correlate. luad<-readr::read_csv(" Read More...
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Bioinformatics
Compare the output from these commands: ls ls -S ls -lh ls -h (when used with -l option, prints file sizes in a human readable format with the unit suffixes: Byte, Kilobyte, Megabyte, Gigabyte, Terabyte. Read More...
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Bioinformatics
11/13/2020 - Register Description: This webinar offers an introduction to FlowJo, an application designed to help with data archiving, analysis, and reporting. The training begins by showing how to load samples and then outlines the steps Read More...
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Bioinformatics
05/21/2020 - Students will learn how to use the point-and-click interface in Partek Flow to go from raw data to experimental results for RNA-Seq analysis. For example: import data from .fastq files; perform QA/QC (Pre-alignment Read More...
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Bioinformatics
05/19/2020 - The class will start with an overview of Partek Genomics Suite with Pathway and followed with the hands-on training on Illumina 450K Methylation array data. The workflow can also be used on HumanMethylation27 (27K) Read More...
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Bioinformatics
08/28/2019 - Please bring your computer with Partek Genomics Suite to this hands-on workshop. You'll need to submit a request through “NCI at Your Service” to obtain the access to this software. Partek will demonstrate Read More...
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Bioinformatics
11/06/2018 - RNA-Seq Data Analysis in Partek Flow Please bring a laptop to this class - or let us know if you need to borrow one. Please printout and bring the handout from the class website. Read More...
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Bioinformatics
12/13/2017 - PLEASE NOTE: This workshop is a BYOC ( Bring Your Own LapTop Computer ) class, and requires installation of Partek Genomics Suite on your laptop ahead of the workshop. Government issued or personal computers are permitted. Read More...
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Bioinformatics
02/05/2013 - This training session will focus on Gene Expression analysis and the rich set of results possible from RNA-Seq based studies. In addition to differential gene expression, researchers also have the opportunity to discover SNPs, Read More...
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Bioinformatics
The strength of ggplot2 is also its weakness. Using a layered approach means that it can often take quite a bit of code to reach a publishable state, even for routine plots. For new users, Read More...
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Bioinformatics
09/13/2023 - Partek Flow is your start-to-finish solution for analyzing high dimensional multi-omics sequencing data. It is a point-and-click software and is suitable for those who wish to avoid the steep learning curve associated with analyzing Read More...
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Bioinformatics
05/18/2022 - Powerful and Intuitive Gene Expression Visualization Tools to Interpret Biological Signals – Bulk and Single Cell Data The increasing use of genomic technologies, such as RNA-Seq and single cell RNA-Seq, to assess gene expression patterns Read More...
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Bioinformatics
10/23/2019 - Please note class will start at 10 AM and end at 12 noon. Thank you! Attention: This class is limited to 28 people due to the size of the room. Please add your name to the waiting Read More...
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Bioinformatics
04/04/2016 - This 2-day course, which includes both lecture and hands-on components, will teach the basic concepts and practical aspects of RNA-Seq Data Analysis. Learn everything from experimental design to statistical analysis. This workshop will include Read More...
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Bioinformatics
12/07/2015 - This 2-day course, which includes both lecture and hands-on components, will teach the basic concepts and practical aspects of RNA-Seq Data Analysis. Learn everything from experimental design to statistical analysis. This workshop will include Read More...
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Bioinformatics
12/01/2015 - REGISTRATION FULL - Please signup for Session 2 December 7/8 This 2-day course, which includes both lecture and hands-on components, will teach the basic concepts and practical aspects of RNA-Seq Data Analysis. Learn everything from experimental Read More...
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Bioinformatics
02/19/2015 - This 2-day course, which includes both lecture and hands-on components, will teach the basic concepts and practical aspects of RNA-Seq Data Analysis. Learn everything from experimental design to statistical analysis. This workshop will include Read More...
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Bioinformatics
After generating and filtering out lower expressing genes from the median ratio normalized expressions data, it is time to perform differential expression analysis to see if there are genes or transcripts (trancripts will be used Read More...
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Bioinformatics
In Lesson 2, I introduced the idea of command options (flags). Command options allow us to change the behavior of a command. Command options, which ultimately allow you to modify command parameters, are extremely important to Read More...
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Bioinformatics
Let's look at a more complicated example using the gene_expression data. We can use ggpubr to explore genes of interest. However, in cases where comparisons are more complicated and faceting is used, this Read More...
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Confocal
2024 Senatorov IS, Bowman J, Jansson KJ, Alilin AN, Capaldo BJ, Lake R, Riba M, Abbey YC, Mcknight C, Zhang X, Raj S, Beshiri ML, Shinn P, Ngyuyen H, Thomas CJ, Corey E, Kelly K. Castrate Read More...
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Confocal
Zeiss LSM 880 NLO Laser Scanning Microscope Laser scanning microscope: 355nm UV laser 405nm, 594nm, 561nm and 633nm diode lasers Argon laser (458nm, 488nm and 514nm lines) Coherent Chameleon Vision II tunable (680nm – 1080nm) IR multiphoton Read More...
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Confocal
2025 Sebastian R, Sun EG, Fedkenheuer M, Fu H, Jung S, Thakur BL, Redon CE, Pegoraro G, Tran AD, Gross JM, Mosavarpour S, Kusi NA, Ray A, Dhall A, Pongor LS, Casellas R, Aladjem MI. Mechanism Read More...
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Confocal
Confocal Microscope Image of Fixed Mouse Embryonic Fibroblast (MEF) Cells Actin proteins (stained red) and Tubulin proteins (stained green) are involved in a number of cellular process such as cell motility, cell division and maintenance Read More...
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Confocal
2024 Date: Tuesday, October 15, 2024 Time and Location: 11 am EST, ZOOM (INVITATION BY LMIG LIST SERVER) Speaker: Dr. Diego Presman (U Buenos Aires) Title: “Insights on Glucocorticoid Receptor Activity Through Live Cell Imaging” Summary: Eucaryotic transcription factors ( Read More...
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Bioinformatics
This lesson provides an introduction to R in the context of single cell RNA-Seq analysis with Seurat. Learning Objectives Learn about options for analyzing your scRNA-Seq data. Learn about resources for learning R programming. Learn Read More...
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Bioinformatics
1. Introduction and Learning Objectives This tutorial has been designed to demonstrate common secondary analysis steps in a scRNA-Seq workflow. We will start with a merged Seurat Object with multiple data layers representing multiple samples that Read More...
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Bioinformatics
Now that we have downloaded the HBR and UHR dataset and know where analysis tools are, let's start learning about RNA sequencing, by first learning about our reference genome and annotation files. Let's Read More...
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Bioinformatics
Now that we have downloaded the HBR and UHR dataset and know where analysis tools are, let's start learning about RNA sequencing, by first learning about our reference genome and annotation files. Let's Read More...
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Bioinformatics
More useful Unix Flags and command options - making programs do what they do Use of wildcards Using tab complete for less typing Access your history with the "up" and "down" Read More...
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Bioinformatics
More useful Unix Flags and command options - making programs do what they do Use of wildcards Using tab complete for less typing Access your history with the "up" and "down" Read More...
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Bioinformatics
More useful Unix Flags and command options - making programs do what they do Use of wildcards Using tab complete for less typing Access your history with the "up" and "down" Read More...
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Bioinformatics
Lesson 4: Useful Unix For this lesson, you will need to login to the GOLD environment on DNAnexus. Lesson 3 Review Biowulf is the high performance computing cluster at NIH. When you apply for a Biowulf account Read More...
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Bioinformatics
Lesson 9: Reference genomes and genome annotations used in RNA sequencing Before getting started, remember to be signed on to the DNAnexus GOLD environment. Lesson 8 Review In Lesson 8, we learned about the basics of RNA sequencing, Read More...
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Bioinformatics
Lesson 9: Reference genomes and genome annotations used in RNA sequencing Before getting started, remember to be signed on to the DNAnexus GOLD environment. Lesson 8 Review In Lesson 8, we learned about the basics of RNA sequencing, Read More...
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Bioinformatics
High resolution single cell profiling assays have provided an unprecedented view of many biological systems and processes, but the spatial context in which this biology is occurring is often crucial. Spatial profiling, including spatial transcriptomic Read More...
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Bioinformatics
03/20/2017 - BTEP Workshop on RNA-Seq Data Analysis (2-day) This 2-day workshop, which includes both lecture and hands-on components, will cover the fundamentals of and best practices for RNA-Seq Data Analysis. Learn everything from experimental design Read More...
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Bioinformatics
10/03/2016 - The maximum number of registrations (25) allowed has been reached for this workshop. You will be put on the waitlist and informed if any cancellations do occur. You are welcome to come on the morning Read More...
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Bioinformatics
09/22/2015 - Learn the basics of microarray gene expression analysis using Partek Genomics Suite and Open Source Tools. As we walk though hands-on analysis of a cancer dataset, you will learn the principles of experimental design, Read More...