Search Results for: Transgene Location

First, let's set up our renv cache location. renv uses a global cache to reduce duplicate installs of packages across projects. When using renv with the global package cache, the project library is instead Read More...

Once we have done this, we can activate renv within our project. But, first, let's verify the location of our renv cache. R renv::paths$cache() # Check the cache location renv::init(bioconductor = "3.16& Read More...

As we learn more about R and start learning our first commands, we will keep a record of our commands using an R script. Remember, good annotation is key to reproducible data analysis. An R Read More...

04/07/2022 - During the first part of the session attendees will learn how to use Partek Flow to perform special transcriptions by overlaying cell location information and histological images on single cell RNA-Seq data using 10X Read More...

07/20/2021 - Register During the first part of the session attendees will learn how to use Partek Flow to perform special transcriptions by overlaying cell location information and histological images on single cell RNA-Seq data using 10 Read More...

11/12/2020 - During the first part of the session attendees will learn how to use Partek Flow to perform special transcriptions by overlaying cell location information and histological images on single cell RNA-Seq data using 10X Read More...

Protein Expression Laboratory The Protein Expression Laboratory develops, improves, and delivers protein-centric services. Our goal is to help client investigators achieve their research goals with the lowest possible cost in the shortest time. All PEL Read More...

Biomarker Discovery with Morphological Context:  Changing how tissue specimens are analyzed < https://youtu.be/mVhfZq8ppbc What is Digital Spatial Profiling? GeoMx Digital Spatial Profiler is a novel platform developed by NanoString. Digital Spatial Read More...

What is Xenium? Xenium is a high-resolution, imaging-based in situ spatial profiling technology from 10x Genomics that allows for simultaneous expression analysis of RNA targets (currently in range of 100’s) within the same tissue section. Read More...

05/07/2025 - Join Dr. Eytan Ruppin, NCI investigator in the Center for Cancer Research, as he discusses Path2Space, a new and unpublished deep learning approach that predicts spatial gene expression directly from histopathology slides. Spatial Read More...

04/16/2025 - Updated Location: ATRF, Frederick MD, Main Auditorium What to bring: &nbsp;Laptop capable of connecting to internet via NIH wifi For questions or to register, please contact Amy Stonelake ( amy.stonelake@nih.gov ) Read More...

11/04/2024 - This one-hour interactive training will cover how to use the ScHARe cloud and Terra platform focusing on how to utilize over 260 social determinants of health (SDoH) data sets and analytic notebooks containing ready-to-use code. Read More...

06/06/2024 - The CCR Genomics Core Facility is pleased to host a virtual technology workshop with EpiCypher on CUT&amp;RUN library prep/sequencing Presentation overview: The location of histone post-translational modifications and chromatin-associated proteins Read More...

Look at the distribution of nCount_RNA with a Violin plot: # set colors cnames% ggplot(aes(color=orig.ident, x=nCount_RNA, fill= orig.ident)) + geom_density(alpha = 0.2) + theme_classic() + scale_x_log10() + geom_vline( Read More...

05/20/2024 - Hybrid (in-person location in Rockville, MD) Virtual attending via&nbsp; WebEx Meeting, link will be available two weeks prior to the meeting date . Attend the&nbsp; 2024 Co-Clinical Imaging Research Resource Program (CIRP) Read More...

Partek Flow is a start-to-finish solution for analyzing high dimensional multi-omics sequencing data. It is a point-and-click software and is suitable for those who wish to avoid the steep learning curve associated with analyzing sequencing Read More...

Course Overview Partek Flow is a start-to-finish solution for analyzing high dimensional multi-omics sequencing data. It is a point-and-click software and is suitable for those who wish to avoid the steep learning curve associated with Read More...

If all R could do was function as a calculator, it wouldn't be very useful. R can be used for powerful analyses and visualizations. As we learn more about R and begin implementing our Read More...

Reshape iris_long to a wide format. Your new column names will contain names from Measurement.location . Your wide data should look as follows: ## # A tibble: 150 × 6 ## Iris.ID Species Sepal.Length Sepal.Width Petal.Length Read More...

Import data from the sheet "iris_data_long" from the excel workbook (file_path = "./data/iris_data.xlsx"). Make sure the column names are unique and do not contain spaces. Save Read More...

Let's open IGV locally on our computer. Then we will copy the Golden Snidget refs folder to our public directory so we can download and use these locally. Remember the location on your computer Read More...

After alignment of sequencing data to genome, we will need to count how many reads aligned to which gene. Using the tool featureCounts, we were able to do this. This tool takes as input our Read More...

Let's submit a batch job. We are going to download data from the Sequence Read Archive (SRA) , a public repository of high throughput, short read sequencing data. We will discuss the SRA a bit Read More...

Variant annotation means predicting the effects of genetic variants (SNPs, insertions, deletions, copy number variations (CNV) or structural variations (SV)) on the function of genes, transcripts, and protein sequence, as well as regulatory regions. The Read More...

Resources Tertiary Analysis - Biological Meaning Pathway Analysis IPA (Qiagen - CCR License) Future talk Functional Analysis Gene Set Enrichment Analysis (GSEA) https://www.gsea-msigdb.org/gsea/index.jsp DAVID https://david.ncifcrf.gov/ Enrichr Read More...

08/22/2023 - In this session, we will discuss conceptual overview of a computing cluster, how to get access to the NCI cluster in Frederick (FRCE) and how to connect and run basic programs. We will also Read More...

08/15/2023 - This talk will cover what to do when you first start looking at a new dataset. We will also discuss how to efficiently orient the data to avoid pitfalls and maximize knowledge gain in Read More...

Each version of R loaded as a module includes a number of installed packages . However, you may want to install additional packages, which will by default be stored in " ~/R/%v/library where %v Read More...

We will create and submit a job script using sbatch that will run the R scripts in the project we created in Lesson 3 ( MyNewProject ). Example job script: nano rjob.sh Paste the following: #!/bin/bash # Read More...

High resolution single cell profiling assays have provided an unprecedented view of many biological systems and processes, but the spatial context in which this biology is occurring is often crucial. Spatial profiling, including spatial transcriptomic Read More...

Coordinate systems are probably the most complicated part of ggplot2. The default coordinate system is the Cartesian coordinate system where the x and y positions act independently to determine the location of each point. --- Read More...

Welcome to our new and improved BTEP Web Site. There have been major changes to the organization and presentation of our content in the hope of making things easier to find. All the content you Read More...

10/21/2022 - Single cell chromatin accessibility, measured by ATAC-seq, and single cell gene expression, measured by RNA-seq provide two views on a cell’s state. From chromatin accessibility it is possible to infer information about the Read More...

If you already have R, check that it is the latest version. On the Mac, launch R from the Applications folder and Check For R Updates under the R menu. If you have the latest Read More...

Installing RStudio Desktop on the Mac We recommend using RStudio Desktop, because it *provides a user-friendly environment with 4 pane views which makes it easier to use for examining data, writing scripts and running R analyses .* Read More...

06/08/2021 - Do you want to know how to use Machine Learning (ML) for accelerating drug discovery? Join us on  June 8, 1:00 pm – 2:00 pm ET,  for the first in a series of workshops on how to use Read More...

01/27/2021 - Please plan to attend the Earl Stadtman Investigator Program search seminar by: Qian Zhu, Ph.D. Dana Farber Cancer Institute/Boston Children's Hospital Dr. Zhu's research interests include: single-cell genomics; spatial transcriptomics; Read More...

01/25/2021 - Presenter: Dr. Arjun Raj from the Perelman School of Medicine, UPenn. Abstract:Anti-cancer therapies can often kill the vast majority of tumor cells but a few rare cells remain and grow despite treatment. Non-genetic Read More...

01/19/2021 - Overview Are you interested in improving your machine or deep learning models? You often cannot be sure you've developed the best model without performing hyperparameter optimization. In this talk, we will explain what Read More...

01/14/2021 - Please plan to attend the Earl Stadtman Investigator Program search seminar by: Noam Auslander, Ph.D. National Center for Biotechnology Information (NCBI), NIH Dr. Auslander's research focus is on designing techniques that make Read More...

01/12/2021 - Please plan to attend the Earl Stadtman Investigator Program search seminar by: Mikhail Kolmogorov, Ph.D. University of California, San Diego Dr. Kolmogorov's research focus is bioinformatics. Particularly, he is interested in algorithms Read More...

01/08/2021 - Abstract: Data sharing is essential for the acceleration of science, but privacy concerns need to be addressed before clinical data can be properly shared for research. I will briefly introduce the main issues in Read More...

12/04/2020 - Register Meeting ID: 161 756 1452 Passcode: 586729 One tap mobile +16692545252,,1617561452#,,,,,,0#,,586729# US (San Jose) +16468287666,,1617561452#,,,,,,0#,,586729# US (New York) Dial by your location +1 669 254 5252 US (San Jose) +1 646 828 7666 US (New York) Meeting ID: 161 756 1452 Passcode: 586729 Find your local number: https://nih.zoomgov.com/ Read More...

11/30/2020 - Register Meeting ID: 918 4307 1125 One tap mobile +13017158592,,91843071125# US (Washington D.C) +19294362866,,91843071125# US (New York) Dial by your location +1 301 715 8592 US (Washington D.C) +1 929 436 2866 US (New York) +1 312 626 6799 US (Chicago) +1 346 248 7799 US (Houston) +1 669 900 6833 US (San Jose) +1 253 215 8782 US (Tacoma) Meeting Read More...

11/16/2020 - Register Cancer heterogeneity is one of the major challenges that hampers the ability to cure the disease. Tumors differ in their genetic profiles and the cellular interactions in the microenvironment, and each tumor may Read More...

11/06/2020 - Register Abstract: The need to integrate knowledge types into big data analytics, generally referred to as explanatory-artificial-intelligence (x-AI), is growing. This talk will describe progress with three approaches to such knowledge enrichment: 1) the use Read More...

08/17/2020 - Speaker: Dr. Mary Carrington The exceptional influence of HLA and related genes located in the MHC on human disease relative to that of variation in the rest of the genome has now been clearly Read More...

The Calendar comes in three variations: The listing of upcoming events (shown on the BTEP home page .) A monthly view of the calendar diplayed in a traditional grid view. A complete listing of all events Read More...

04/25/2018 - Join us for a seminar/webinar session where Partek Specialist will show you how to analyze your Single Cell RNA-Seq data in an intuitive, interactive and powerful way using Partek Flow. The solution simplifies Read More...

04/26/2017 - The CCR Bioinformatics Training and Education Program (BTEP) is pleased to organize a workshop on Partek Genomics Suite for scientists at NCI-Frederick. Partek ® Genomics Suite ® is a versatile scientific software with an easy-to-use graphical Read More...

04/25/2017 - This talk will be an introduction to Methyl-seq, where you will learn about leveraging the power of next-generation sequencing (NGS), both genome-wide and targeted approaches, that can provide insight into methylation patterns at a Read More...

Back Services: Biophysics Facility offers DSC as an open-access instrument. First-time users must complete training before gaining access to the instrument reservation calendar. Location: Building 50, room 3123 Description: The differential scanning calorimeter measures the constant pressure Read More...

Back Services: Biophysics Facility offers Tycho as an open-access instrument.  This instrument is very easy to use, and no formal training is required.  Core staff will help with their first experiment of new Read More...

Back Services: Biophysics Facility offers MP as an open-access instrument.  First-time users must complete a short training session before gaining access to the instrument training calendar.  Training includes mass distribution analysis of a Read More...

Back Services: Biophysics Facility offers fluorometers as open-access instruments.  First-time users must complete a short training session before gaining access to the instrument reservation calendar. Location: Building 50, room 3226 Description: Some substances reemit light after Read More...

Back Services: This instrument is not user accessible.  We provide both data collection and data analysis services. Location: Building 50, room 3331 Description: An analytical ultracentrifuge is equipped with absorption and interference optical systems that monitor Read More...

Back Services: Biophysics Facility offers ZetaView as an open-access instrument. First-time users must complete a short training session before using it for the first time. Training includes instrument calibration and size analysis of a standard Read More...

Back Services: Biophysics Facility offers MDS as an open-access instrument. First-time users must complete a short training session before gaining access to the instrument reservation calendar. Training includes the KD determination of a standard molecular Read More...

Back Services: Biophysics Facility offers Octet as an open-access instrument.  First-time users must complete a short training session before gaining access to the instrument reservation calendar.  Training includes a full analysis of a Read More...

Back Services: Biophysics Facility offers ITC calorimeters as open-access instruments.  First-time users must complete a short training session before gaining access to the instrument reservation calendar.  Training includes performing a test experiment and Read More...

Back Services: Biophysics Facility offers MST as an open-access instrument.  First-time users must complete a short training session before gaining access to the instrument reservation calendar.  Training includes the KD determination of a Read More...

Back Services: Biophysics Facility offers DLS as an open-access instrument. First-time users must complete a short training session before gaining access to the instrument reservation calendar. Training includes DLS analysis of small- and large-molecular size Read More...

Services: Biophysics Facility offers CD as an open-access instrument.  First-time users must complete a short training session before gaining access to the instrument reservation calendar. Location: Building 50, room 3123 Description: CD spectroscopy measures the difference Read More...

Back Services: Biophysics Facility offers CD as an open-access instrument.  First-time users must complete a short training session before gaining access to the instrument reservation calendar. Location: Building 50, room 3123 Description: CD spectroscopy measures the Read More...

Back Services: We offer a limited sample processing service using standard SEC-MALS and FFF protocols.  This service is intended for the occasional users of this system.  Researchers who expect to use this instrument Read More...

Back Services: We offer a limited sample processing service using standard SEC-MALS and FFF protocols.  This service is intended for the occasional users of this system.  Researchers who expect to use this instrument Read More...

Register now for an upcoming seminar on January 22, 2025 , “Revealing Tumor-Promoting Signals in Breast Cancer Subtypes: A NIDAP Analysis of Single-Cell Data” . This one-hour talk will feature guest speaker and NIDAP developer, Josh Meyer, who will Read More...

Learning Objectives This tutorial was designed to demonstrate common secondary analysis steps in a scRNA-Seq workflow. We will start with a merged Seurat Object with multiple data layers representing multiple samples. Throughout this tutorial we Read More...

Learning Objectives To understand: 1. the difference between R and RStudioIDE. 2. how to work within the RStudio environment including: creating an Rproject and Rscript navigating between directories using functions obtaining help how R can enhance data Read More...

Help Session Lesson 3 Loading data Import data from the sheet "iris_data_long" from the excel workbook (file_path = "./data/iris_data.xlsx"). Make sure the column names are unique and Read More...

Which of the following will throw an error and why? 4 _ chr :1:2: unexpected input ## 1: 4_ ## ^ . 4 chr :1:3: unexpected symbol ## 1: .4chr ## ^ {{Edet}} Create the following objects; give each object an appropriate name (your best guess at what name to Read More...

This is our first coding help session. We have designed some practice problems to get you acquainted with using R before beginning to wrangle in our next lesson. Practice problems Which of the following will Read More...

R Crash Course: A few things to know before diving into wrangling Learning the Basics Objectives 1. Learn about R objects 3. Learn how to recognize and use R functions 4. Learn about data types and accessors Console Read More...

fastq-dump and fasterq-dump can be used to download FASTQ-formatted data. Both download the data in SRA format and convert it to FASTQ format. fastq-dump SRR1553607 creates the file: SRR1553607.fastq Check the file to make Read More...

fastq-dump and fasterq-dump can be used to download FASTQ-formatted data. Both download the data in SRA format and convert it to FASTQ format. fastq-dump SRR1553607 creates the file: SRR1553607.fastq Check the file to make Read More...

In this portion of the class, it is very important that you have IGV already opened on your computer. See Figure 1 and Figure 2 on how to load the relevant alignment outputs for HBR_1 and UHR_1 Read More...

For visualizing the HBR and UHR alignment results, we will use the built in Human hg38 genome. To do this, we will just goto the genome selection box and select hg38 (Figure 2). In Figure 3, we Read More...

Let's download the data and learn how to decompress it. First, we will create a place to store the data. Go to the directory you created for working with class material. If you haven' Read More...

In lesson 9, we learned that reference genomes came in the form of FASTA files, which essentially store nucleotide sequences. In this lesson, we will learn about the FASTQ file, which is the file format that Read More...

Lesson 10: Introducing the FASTQ file and assessing sequencing data quality Before getting started, remember to be signed on to the DNAnexus GOLD environment. Lesson 9 Review In the previous lesson, we explored the reference genomes and Read More...

This page uses content directly from the Biostar Handbook by Istvan Albert. Learn: using trimmomatic to remove low-quality bases from a sequence Always remember to activate the bioinformatics environment. conda activate bioinfo We will be Read More...

This page uses content directly from the Biostar Handbook by Istvan Albert. Learn: using trimmomatic to remove low-quality bases from a sequence Always remember to activate the bioinformatics environment. conda activate bioinfo We will be Read More...

Lesson 9 Practice Objectives In this practice session, we will apply our knowledge to learn about the reference genome and annotation file for the Golden Snidget dataset visualize the Golden Snidget genome using the Integrative Genome Read More...

Database for Annotation, Visualization and Integrated Discovery (DAVID) - an overview Before getting started, remember to be signed on to the DNAnexus GOLD environment. Lesson 17 review In the previous class, we got an overview of Read More...

Here, let's change back in the ~/biostar_class/hbr_uhr/hbr_uhr_hisat2 folder. cd $hbr_uhr_hisat2 To align FASTQ files for one sample, we construct the HISAT2 command with the following options Read More...

To align FASTQ files for one sample, we construct the HISAT2 command with the following options. The "-x" flag prompts us to enter the base name (ie. without extension) of genome index. The Read More...

Lesson 5: Working on Biowulf Lesson 4 Review Flags and command options Wildcards ( * ) Tab complete Accessing user history with the "up" and "down" arrows cat , head , and tail Working with file content (input, Read More...

This page uses context taken directly from the Biostar Handbook by Istvan Albert. Remember to activate the class bioinformatics environment. conda activate bioinfo Introduction to Genomic Variation Genomic variations are typically categorized into different classes Read More...

Lesson 14: Visualizing alignment results Before getting started, remember to be signed on to the DNAnexus GOLD environment. Lesson 13 Review Previously, we used the application HISAT2 to align the raw sequencing data from the Human Brain Read More...

Lesson 7: Downloading the RNA-Seq Data and Dataset Overview Lesson Review pwd (print working directory) ls (list) touch (creates an empty file) nano (basic editor for creating small text files) using the rm command to remove Read More...

Lesson 2: Navigating file systems with Unix Quick review Unix is an operating system We use a unix shell (typically bash) to run many bioinformatics programs We need to learn unix to use non-GUI based tools Read More...

Lesson 14: Visualizing alignment results Before getting started, remember to be signed on to the DNAnexus GOLD environment. Lesson 13 Review Previously, we used the application HISAT2 to align the raw sequencing data from the Human Brain Read More...

Lesson 6: sra-tools, e-utilities, and parallel This page uses some content directly from the Biostar Handbook by Istvan Albert. Lesson 5 Review: The majority of computational tasks on Biowulf should be submitted as jobs: sbatch or swarm Read More...

Lesson 6: Downloading data from the SRA For this lesson, you will need to login to the GOLD environment on DNAnexus. Lesson 5 Review: The majority of computational tasks on Biowulf should be submitted as jobs: sbatch Read More...

Database for Annotation, Visualization and Integrated Discovery (DAVID) - an overview Lesson 17 review In the previous class, we got an overview of functional and pathway analysis, which help to put RNA sequencing results into biological Read More...

Lesson 13: Aligning raw sequences to reference genome Before getting started, remember to be signed on to the DNAnexus GOLD environment. Lesson 11 Review In Lesson 11 we learned to aggregate multiple FASTQC reports into one using MultiQC, Read More...

Lesson 13: Aligning raw sequences to reference genome Before getting started, remember to be signed on to the DNAnexus GOLD environment. Lesson 11 Review In Lesson 11 we learned to aggregate multiple FASTQC reports into one using MultiQC, Read More...

Lesson 16: RNA sequencing review and classification based analysis Before getting started, remember to be signed on to the DNAnexus GOLD environment. Review In the previous classes, we learned about the steps involved in RNA sequencing Read More...

Lesson 3: R Project Management and renv Learning objectives Discuss the importance of reproducibility Learn ways to make R analyses more reproducible Learn how to set up and organize an R project Learn how to use Read More...

Lesson 2: Getting Started with R on Biowulf Learning objectives Understand how R can be deployed on Biowulf Understand how to access and use R modules Learn to create a custom R library on Biowulf Deploying Read More...

Lesson 4: Submitting R Scripts via command line Learning Objectives Learn how to use R with less interaction Learn how to deploy sbatch R jobs, and learn about alternatives such as swarm . Learn about R job Read More...

Scatter plots and plot customization Objectives Learn to customize your ggplot with labels, axes, text annotations, and themes. Learn how to make and modify scatter plots to make fairly different overall plot representations. Load a Read More...

Introduction to R and RStudio IDE Objectives To understand: 1. the difference between R and RStudioIDE. 2. how to work within the RStudio environment including: creating an Rproject and Rscript navigating between directories using functions obtaining help 3. Read More...

10/19/2022 - Speaker: Ron Germain, M.D., Ph.D. NIH Distinguished Investigator NIAID, NIH Dr. Germain received his Sc.B. and Sc.M. from Brown University in 1970 and his M.D. and Ph.D. from Harvard Read More...

02/08/2021 - Dear colleagues, Coming Monday, Feb 8th, we'll be having a guest lecture by Prof. Vineet Bafna from UCSD. Abstract: Increase in the number of copies of tumor promoting (onco-) genes is a hallmark Read More...

12/07/2020 - Register Meeting ID: 918 4307 1125 One tap mobile +13017158592,,91843071125# US (Washington D.C) +19294362866,,91843071125# US (New York) Dial by your location +1 301 715 8592 US (Washington D.C) +1 929 436 2866 US (New York) +1 312 626 6799 US (Chicago) +1 346 248 7799 US (Houston) +1 669 900 6833 US (San Jose) +1 253 215 8782 US (Tacoma) Meeting Read More...

09/04/2020 - Speakers: Dave Clements, Galaxy Community Manager, Johns Hopkins University, Steven Weaver, Senior Programmer Analyst, Temple University Galaxy is an open web-based platform for data integration and analysis in the life sciences. Galaxy makes sophisticated Read More...

06/19/2017 - Harvesting the Wealth of TCGA Data The Cancer Genome Atlas (TCGA) is a large-scale study that has catalogued genomic data accumulated from more than 20 different types of cancer including mutations, copy number variation, mRNA Read More...

04/17/2017 - Probing DNA-Protein Interactions This 2-day workshop, which includes both lecture and hands-on components, will teach the basic concepts and practical aspects of ChIP-Seq data analysis. Topics to be covered include: experimental design; read alignment; Read More...