Proteins and Proteomics

The Antibody Engineering Program (AEP) is located at the Laboratory of Molecular Biology, which is part of the Center for Cancer Research (CCR), an intramural program at the National Cancer Institute (NCI). AEP focuses on generating therapeutic antibodies using phage display technology, including single-domain antibodies (also commonly known as ‘nanobodies’). Antibody-based therapeutics have emerged as a major component in clinical applications, and single-domain antibodies have shown promise in targeting difficult antigens and buried sites. Dr. Mitchell Ho and colleagues at the NCI … [learn more]

Protein Characterization Laboratory (PCL) offers various technologies to CCR investigators to characterize proteins and metabolites. The laboratory develops and applies state-of-the-art analytical technologies, primarily mass spectrometry, liquid chromatography, and Surface Plasmon Resonance (SPR), to advance the understanding of cellular function at the protein, proteomics, metabolite, and metabolomic levels. PCL engages in both short and long-term collaborations based on the need of each project. PCL is operated by Leidos Biomedical Research Inc. on behalf of NCI as part of the Frederick Nat… [learn more]

The Protein Expression Laboratory produces proteins to help CCR investigators achieve their research goals with the lowest possible cost in the shortest time. PEL is operated by Leidos Biomedical Research Inc. on behalf of NCI as part of the Frederick National Laboratory. Established Technologies: Clone construction for protein expression, Expression in microbial, insect, and mammalian cells, Small-scale parallel expression and purification testing, Protein purification from micrograms to grams, [learn more]

The Spatial Imaging Technology Resource (formerly the Nanoscale Protein Analysis Section of the Collaborative Protein Technology Resource or CPTR) provides expertise and service in state-of-the-art protein analysis technologies to advance CCR research in basic discovery and translation/clinical studies. Applications include: Quantitative profiling of cell signaling and post-translational modification, Measurement of cytokine, metabolite and serum/plasma markers , Identification and validation of biomarkers & therapeutic targets, Assessment of on‐ and off‐target drug activity and … [learn more]

The Mass Spectrometry Unit of the Collaborative Protein Technology Resource uses mass spectrometry for the study of proteins. We perform collaborative experiments with CCR researchers in areas such as: interactomes, quantitative global proteome analyses, and identification of sites of post-translational modification. In addition, we perform structural proteomics analyses. Finally, we also have an ICP-MS for analysis of inorganic atoms. Established Technologies: Mass Spectrometry Characterization of protein or RNA interactomes, Identification of proteins in complexes, org… [learn more]