Proteins and Proteomics

The Biological Products Core provides the AIDS research community with high-quality purified preparations of various strains of Human Immunodeficiency Virus (HIV) and Simian Immunodeficiency Virus (SIV), economically prepared by leveraging the economy of scale. Materials provided include chemically inactivated virus prepared by treatment with Aldrithiol-2. This chemical inactivation method was developed by AIDS and Cancer Virus Program personnel and eliminates infectivity while preserving functional surface proteins, including envelope glycoproteins. This property makes them attractive prepara… [learn more]

Protein and Metabolite Characterization Core (PMCC), formerly known as the Protein Characterization Lab (PCL),  offers various technologies to CCR investigators to characterize proteins and metabolites. The core develops and applies state-of-the-art analytical technologies, primarily mass spectrometry, and liquid chromatography, to advance the understanding of cellular function at the protein, proteomics, metabolite, and metabolomic levels. It engages in both short and long-term collaborations based on the need of each project. It is operated by Leidos Biomedical Research Inc. on behalf of NC… [learn more]

The Mass Cytometry Core (MCC) provides access to cutting-edge platforms and expert technical support for high-dimensional single-cell analysis and spatial proteomic profiling. A central expertise of the core is Imaging Mass Cytometry (IMC), which allows simultaneous quantification of dozens of protein markers in intact tissue sections at single-cell resolution while preserving spatial context. This approach enables in-depth analysis of tissue architecture, micro-environments, and cellular interactions. In addition, the MCC provides suspension mass cytometry (CyTOF) for high-parameter profiling… [learn more]