CCR Mass Spectrometry Resource

The CCR Mass Spectrometry Resource uses mass spectrometry for the study of proteins. We perform collaborative experiments with CCR researchers in areas such as: interactomes, quantitative global proteome analyses, and identification of sites of post-translational modification. In addition, we perform structural proteomics analyses. Additionally, we develop targeted quantitative assays for proteins and small molecules. Finally, we have an ICP-MS for analysis of inorganic atoms.

Established Technologies

Mass Spectrometry

• Characterization of protein or RNA interactomes
• Identification of proteins in complexes, organelles, subcellular fractions
• Global protein quantitation (label-free, SILAC, TMT, reductive dimethylation)
• Characterization of sites of post-translational modification, including phosphorylation, acetylation, methylation, glycosylation and ubiquitination
• Targeted quantitation of proteins and small molecules
• Cross-linking mass spectrometry
• Limited proteolysis mass spectrometry
• ICP-MS analysis of inorganic atoms and compounds
• Determination of accurate mass of intact proteins

Developing Technologies

Mass Spectrometry

• Quantitation of RNA nucleosides
• Intracellular cross-linking