Proteins and Proteomics

The Antibody Characterization Laboratory (ACL) is the laboratory responsible for the development of well-characterized monoclonal antibody reagents. The NCI’s Office of Cancer Clinical Proteomics Research funds ACL as a resource to the entire cancer research community. ACL produces monoclonal antibodies to putative cancer targets selected by an Antibody Scientific Committee, and each antibody is characterized using optimized Standard Operating Procedures (SOPs). Approximately once a year there is a reagent target request open to the extramural research community. ACL is operated by Lei… [learn more]

The Antibody Engineering Program (AEP) is part of the Center for Cancer Research (CCR) at the National Cancer Institute (NCI). The program focuses on generating therapeutic antibodies by phage display technology, including single domain antibodies (also known as ‘nanobodies’). While antibody-based therapeutics have emerged as a major component in clinical applications, the generation of antibodies to important targets such as cell surface receptors, ion channels, and glycoproteins remains challenging. Single domain antibodies have shown a promising ability to target difficult antigen… [learn more]

The Spatial Imaging Technology Resource (formerly the Nanoscale Protein Analysis Section of the Collaborative Protein Technology Resource or CPTR) provides expertise and service in state-of-the-art protein analysis technologies to advance CCR research in basic discovery and translation/clinical studies. Applications include: Quantitative profiling of cell signaling and post-translational modification, Measurement of cytokine, metabolite and serum/plasma markers , Identification and validation of biomarkers & therapeutic targets, Assessment of on‐ and off‐target drug activity and … [learn more]

Bruker AVANCE 400 and 500 MHz NMR instruments. Helium Cryoprobe technology on the 500 MHz machine for added sensitivity, especially for Carbon-13 spectra. Access to a second 500 MHz instrument with Prodigy  Liquid Nitrogen-cooled cryoprobe . User Accounts can be created for automated setup and experiment acquisition.  Use of ICONNMR automated software with 24-slot sample changers on each machine. User training available. Some service to those who are unfamiliar with NMR can be performed either as a one-time aid for compound identification or as a collaborative project for more extensive … [learn more]

The primary objective of the Protein Technology Core (PTC) is to establish an experimental screening pipeline for single-domain antibodies termed nanobodies. Nanobodies are antibody fragments consisting of a single monomeric variable antibody domain. The PTC, which has recently undergone renovation, is equipped with state-of-the-art equipment to enable efficient screening of synthetic libraries utilizing ribosome display technologies and those derived from the immunization of alpacas. Enrichment of selection can be easily monitored by qPCR using QuantStudio5 for synthetic and immunogenic nano… [learn more]

The Mass Spectrometry Unit of the Collaborative Protein Technology Resource uses mass spectrometry for the study of proteins. We perform collaborative experiments with CCR researchers in areas such as: interactomes, quantitative global proteome analyses, and identification of sites of post-translational modification. In addition, we perform structural proteomics analyses. Finally, we also have an ICP-MS for analysis of inorganic atoms. Established Technologies: Mass Spectrometry Characterization of protein or RNA interactomes, Identification of proteins in complexes, org… [learn more]