The Mouse Modeling Core assists NIH investigators by generating and preserving genetically-engineered mouse strains. Services include scientific consultation, gene-targeting in mouse embryonic stem cells, micro-injection of nucleic acids, proteins, or ES cells into mouse embryos, in vitro fertilization, cryopreservation of germplasm, and regeneration of mice from frozen stocks. The MMC also interfaces with the Genome Modification Core to generate genetically or epigenetically-altered lines of mice using CRISPR and other nuclease-based methods (TALENS, Zn Fingers).
The NCI Mouse Repository (NMR), operated by the MMC, is an NCI-funded resource for mouse cancer models and associated strains distributed as cryopreserved germplasm to academic, non-profit, and commercial scientific organizations. In addition, the NMR carries 1501 genetically engineered mouse embryonic stem cells lines were generated by the Cold Spring Harbor (supported by the Division of Cancer Biology), harboring conditional microRNA transgenes, available to the scientific community to help address the role that microRNAs play in human cancers, their use as diagnostic tools, and their potential functions as targets for therapeutic intervention in the treatment of cancer.
All NCI Mouse Repository mice are free, however, the recipient is responsible for shipping costs. Cryo-archived strains are supplied as frozen embryos. Recovery of live mice from the Repository may be requested by interfacing with the Mouse Modeling Core.
To request MMC services from this CCR core facility, you must submit your requisition through NAS.