NATIONAL CANCER INSTITUTE - CANCER.GOV

Contact Information


Primary Contact

Parirokh “Roackie” Awasthi
Director

Location

Building 469, Room 205
1050 Boyle Street
Frederick, MD 21701

Overview

Mouse models of human cancer have had a profound impact on our current understanding of the mechanisms of tumorigenesis and the pathways regulated by cancer-related genes. These models hold the promise of serving as critical tools in the discovery and testing of novel therapeutics to be used in the treatment and prevention of cancer.

The Transgenic Mouse Model Laboratory (TMML) provides assistance to the scientific community with the generation of genetically engineered mice (GEM), offering a complete array of services aimed at the successful generation of transgenic and gene-targeted mice, and their expansion and cryopreservation.

Capabilities

  • Transgenic Mouse Production
  • Microinjection services
  • GEMM production utilizing CRISPR/Cas Technology
  • Cryopreservation
  • Reconstitution from Cryopreserved Germplasm
  • Strain Rescue
  • Colony Expansion by In-Vitro Fertilization (IVF)
  • Rederivation to SPF Health status

User Guidelines

All NCI Mouse Repository mice are free, however, the recipient is responsible for shipping costs. Cryo-archived strains are supplied as frozen embryos. Recovery of live mice from the Repository may be requested by interfacing with the Mouse Modeling Core.

To request MMC services from this CCR core facility, you must submit your requisition through NAS.

Keywords

12T-1012T-7f68 T2/Onc (Sleeping Beauty)76 T2/Onc (Sleeping Beauty)Ahr nullAkt1Apc CKOApc Delta 580Apc1638AprtArf floxedArf-null (p19)ArfGFPBLBP-creBloom homolog mutantBrca1 Exon 11 delBrca1 KOBrca1 floxed (FVB;129)Brca1 floxed (STOCK)Brca1 nullBrca2 floxedC57BL/6 DNTGFRIICAG-LSL-EGFR-WTCAG-LSL-EGFRvIIICAGGS-SB10 (Sleeping Beauty)DMP-1 nullE2f3E2f4ED-L2/CreEGFR-L858RFabp1-CreFen1GFAP-TV-AGFAP-hCDK4Gadd45a nullHB-PLAPHB-PLAP(A to G)HB-Plap(G11)HPV16-E6Hi-MycInk4a/Arf null (B6)Ink4a/Arf null (FVB)K-rasLA1K-rasLA2K14 CreK14-HPV16K5/tTALANALPV-TAgLSL K-ras G12DLST1135LST1137Lambda-MYCLangerin-CreLine FLkb1 floxLo-MycMH19MMTV-CreMMTV/c-mycMPAKTMdm-2floxMdm-2puroMdm-2recMen1 nullMen1deltaN3-8MicroRNAMlh1 nullMsh2 nullMsh3 nullMsh6 nullMyf6-ires-cre knock-inN-ras nullNf1 floxNf1 null (129)Nf1 null (B6)Nkx3.1 null (B6)Nkx3.1 null (B6;129)Nkx3.1 null (FVB)Nkx3.1-CreNkx3.1-CreERT2Nras KONtv-aP190 BCR-ABLPB-Cre4PB-FGF8b-line 3PML nullPML-RARaPTENPax3:Fkhr conditional knock-inPax7(CreERp)Pdx-1-creRARa-PMLRIP1-Tag2 (B6)RIP1-Tag2 (C3)RIP1-Tag5ROSA-CreERROSA26-pCAGGs-LSL-LuciferaseRb floxedRb1 nullRosa LSL SBase/TG12740Rosa LSL SBase/TG12775Rosa LSL SBase/TG6070Rosa LSL SBase/TG6113RosaSBSV11SWR Brca2 exon 27 delSprr2f-CreT121TG-E6/E7TG6070TG6113TH-MYCNTdn/11Tet-inducible systemTetO-HRASTg p53 R172HTgfb1 Rag2 nullTgfbr2 flox (FVB)Tsc1Tyr-HRASTyr-rtTAVillin-CreWAP-CreWAP-TagWAP-rtTA-CreWAPT121XPV knockoutdl1135dl1137Animal ResourceshGFAP-CreiDCT-GFPiMycEunci-corep16(Ink4a) nullp19(Arf) null (B6;129)p19(Arf) null (FVB)p53 LSL R172Hp53 LSL R270Hp53 R172Hp53 R270Hp53 floxeds100B-v-erbB (Ink4a-Arf +/-)vil-Cre Fo20CRISPR/cas9CryoCryopreservationCytoplasmic MicroinjectionEmbryoGEMMGenetically EngineeredIn-Vitro FertilizationIVFMouse ModelPronuclear MicroinjectionRecoveryRederivationSperm CryopresevationStrain RecoveryTransgenic