Bethesda, MD
Core Facility
The Flow Cytometry Core (LGI) offers established technologies to support studies using flow cytometry and cell sorting. Established Technologies Applications that run on FACS Caliburs include: Immunophenotyping (up to 4-color), Intracellular markers, including cytokines and Read More...
Frederick, MD
Core Facility
The CCR-Frederick Flow Cytometry Core Facility provides research support to the Frederick-CCR community, including cytometry analysis and sorting services, instrument maintenance, new user training, and technical consultation. Typical Assays Performed by Core Instruments Immunophenotyping of Read More...
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HALO HALO is a powerful 2D image analysis tool with AI capabilities to perform annotations and analysis on bright-field and immunofluorescent images for digital pathology and numerous other applications. It offers a set of algorithms Read More...
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FRET (Fluorescence Resonance Energy Transfer) FRET is a light microscopy method for detecting protein‐protein interactions within intact cells. FCS (Fluorescence Correlation Spectroscopy) Fluorescence correlation spectroscopy (FCS) is a correlation analysis of fluctuation of the Read More...
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empanada v1.2 now released empanada-napari now comes with: MitoNet v1, NucleoNet v0.1 and DropNet v0.1(and other new useful modules) Empanada (EM PANoptic Any Dimension Annotation) is a tool Read More...
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Training Contact the LCBG Microscopy Core to discuss your experiment: Meet with Ross Lake, Core Head, to discuss your specific project. If the project is feasible, schedule an appointment to receive training and use the Read More...
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iLab The Laboratory of Cancer Biology and Genetics Microscopy Core is using a new system to reserve a microscope. Click the button below to access iLab.
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2024 Senatorov IS, Bowman J, Jansson KJ, Alilin AN, Capaldo BJ, Lake R, Riba M, Abbey YC, Mcknight C, Zhang X, Raj S, Beshiri ML, Shinn P, Ngyuyen H, Thomas CJ, Corey E, Kelly K. Castrate Read More...
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Techniques The Laboratory of Cancer Biology and Genetics Microscopy Core houses multiple systems that can be used to analyze cell structure, protein expression, and cell dynamics using immunofluorescence. These include inverted epifluorescence microscopes, a confocal Read More...
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Contact the LCBG Microscopy Core to discuss your experiment Please login to the Bookitlab with your NIH credentials and register as user. Once you have registered on the website, coordinate a Read More...
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Zeiss LSM980 with Airyscan 2 Confocal Microscope Inverted microscope Two PMTs and four GaAsP detectors for confocal imaging 4 color Airyscan 2 “super-resolution” detector Airyscan Multiplex modes for increased acquisition speed with high sensitivity 5x, 10x, 20x, 40x, 63 Read More...
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Our Team Tatiana S. Karpova Ph.D.Core Headkarpovat@nih.govBuilding 41, Room C615240-760-6637 David A. Ball Ph.D.Core Biologistballa@nih.govBuilding 41, Room B114D240-760-6577 Mohamadreza Fazel, Ph.D.Core Biologistmohamadreza. Read More...
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Software Image Acquisition Commercial imaging systems of LRBGE Optical Microscopy Core are controlled by acquisition software specifically designed for the appropriate microscope, such as ZEN (Zeiss confocal microscopes), Nikon Elements (Nikon), Imspector (Abberior). Custom-built HILO Read More...
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General Usage Policies Principal investigators should read this document and sign it. PI’s/postdocs should attach a short-written summary of the project to the signed doc. If the project changes, Read More...
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WIDE-FIELD MICROSCOPY Nikon Ti 2000 wide-field microscope Capabilities: This microscope is suited for live cell imaging (4D) and fixed cells (3D) imaging. This microscope produces high quality wide-field images that may be improved by Read More...
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Software LAS Leica Application Suite X (LAS X) is the one software platform for all Leica microscopes: It integrates confocal, wide field, stereo, super-resolution, and light-sheet instruments from Leica Microsystems. MetaMorph The MetaMorph® Microscopy Automation Read More...
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Leica SP8 LSCM with white light laser The SP8 LIGHTNING confocal microscope allows you to make proper and detailed observations of fast biological processes. Your experimental work will have the benefit of super-resolution, high-speed imaging, Read More...
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Macro’s for Image Processing Dr. Wisniewski wrote a number of custom ImageJ Macros aimed at streamlining image processing tasks such as: Software Imaris The world’s leading Interactive Microscopy Image Analysis software company, actively Read More...
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Zeiss LSM 880 NLO Laser Scanning Microscope Laser scanning microscope: 355nm UV laser 405nm, 594nm, 561nm and 633nm diode lasers Argon laser (458nm, 488nm and 514nm lines) Coherent Chameleon Vision II tunable (680nm – 1080nm) IR multiphoton Read More...
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Gianluca Pegoraro, PhD gianluca.pegoraro@nih.gov Facility Head 240-760-6696 Bldg. 41, Room B909 Dr. Pegoraro received his Ph.D. in Molecular Genetics from the International School of Superior Studies in Trieste, Italy in 2004. After Read More...
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HiTIF Protocols Cell Fixation PFA Cell Fixation with the Blue Washer Cell Seeding Seeding Cells in the imaging plates using the Multidrop Combi Functional Genomic Screens RNAi/CRISPR-KO Screen Assay Development SOP siRNA/sgRNA Spotting Read More...
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Image Analysis Signals Imaging Artist Next generation image analysis and management platform for high-content screening and cell imaging data. Quickly process, analyze, share, and store the vast volumes of data. Cell Profiler CellProfiler is free, Read More...
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2025 Sebastian R, Sun EG, Fedkenheuer M, Fu H, Jung S, Thakur BL, Redon CE, Pegoraro G, Tran AD, Gross JM, Mosavarpour S, Kusi NA, Ray A, Dhall A, Pongor LS, Casellas R, Aladjem MI. Mechanism Read More...
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A typical HTI screening project at HiTIF can be subdivided in 5 phases: Pre-Development The investigator contacts the Facility Head to inquire about the current availability of instrumentation time and manpower for the project to be Read More...
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Yokogawa CV8000 High-throughput spinning disk confocal microscope The Yokogawa CV8000 is HiTIF second high-throughput imaging system. The CV8000 feature set is similar to the CV7000.When compared to the latter, the CV8000 has a newer Read More...
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Stephen Lockett, Ph.D. Director, OMAC locketts@nih.gov 301-846-5515 Valentin Magidson, Ph.D. Scientist magidsonv@mail.nih.gov 301-846-6092 Will Heinz, Ph.D. Scientist heinzwf@nih.gov 301-846-1239 OMAC Read More...
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Confocal Microscope Image of Fixed Mouse Embryonic Fibroblast (MEF) Cells Actin proteins (stained red) and Tubulin proteins (stained green) are involved in a number of cellular process such as cell motility, cell division and maintenance Read More...
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2024 Coutinho, L. L., Femino, E. L., Gonzalez, A. L., Moffat, R. L., Heinz, W. F., Cheng, R. Y. S., Lockett, S. J., Rangel, M. C., Ridnour, L. A. & Wink, D. A. NOS2 and Read More...
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Image Analysis Capabilities: Custom Development Software Supported Software Platforms Arivis Vision 4D Arivis is software for image processing, analysis, and display for working with multi-channel 2D, 3D and 4D images of almost unlimited size independent Read More...
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Training We recommend that users become familiar with the principals of fluorescence labeling and optical microscopy before arranging for training. We recommend the following sites for learning about microscopy: Introductions to Fluorescence Microscopy Fluorescence Labeling Read More...
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ZEISS Elyra 7 with Lattice SIM² Type: Wide-field structured illumination microscope Capabilities: Super resolution 2D and 3D imaging of live or fixed cells Apotome SIM: 170 nm lateral, 450 nm axial Apotome SIM2: 140 nm lateral, 300 nm axial Lattice Read More...
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Research Mission The goal of OMAC’s research is to understand molecular mechanisms driving carcinogenesis and the reversal of this process through treatment, by utilization and advancement of optical microscopy techniques. These techniques include Read More...
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Our Team Michael Kruhlak, Ph.D.Core Headkruhlakm@nih.govBuilding 37, Room B114E240-858-3342 Dr. Kruhlak received his Ph.D. from the Department of Biochemistry and Molecular Biology at The University of Calgary Medical Read More...
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Image Processing Capabilities: Please contact Michael Kruhlak if you have any questions regarding image analysis.
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iLab The NCI Microscopy Core is using a new system to reserve a microscope. Use this link to access the iLab software. Training Contact the CCR Microscopy Core to Discuss your Experiment: Registration and Making Read More...
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2024 Mizunuma M, Redon CE, Saha LK, Tran AD, Dhall A, Sebastian R, Taniyama D, Kruhlak MJ, Reinhold WC, Takebe N, Pommier Y. Acetalax (Oxyphenisatin Acetate, NSC 59687) and Bisacodyl Cause Oncosis in Triple-Negative Breast Read More...
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Software Several software packages are available to users Arivis Vision 4D Arivis Vision4D is a modular software for working with multi-channel 2D, 3D and 4D images of almost unlimited size independent of Read More...
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Contact the CCR Microscopy Core to discuss your experiment Thank you for your interest in using the CCR Microscopy Core. Please login to the iLab with your NIH credentials and register as user. Once you Read More...
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Nikon SoRa Spinning Disk Capabilities: Inverted microscope Photo-metrics BSI sCMOS camera Yokogawa SoRa CSU-W1 spinning disk unit Super-resolution, confocal and wide-field imaging modes 4x, 10x, 20x and 60x objective lenses Mad City Labs 500 mm piezo Read More...
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General Microscopy Resources Confocal Listserv Email discussion list focused on confocal microscopy, but also including topics on fluorescence microscopy and digital imaging. MicroscopyU Online learning platform by Nikon. An online source for Microscopy education. Leica Read More...
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Optical Microscopy Core of the Experimental Immunology Branch (CCR/NCI) announces the introductory classes to Image J/FIJI: December 16th and 18th, 2020, 10 am to 4 pm with 1 hr break. Dr. Jan Wisniewski “Streamline Your Image Analysis: Read More...
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2024 Date: Tuesday, October 15, 2024 Time and Location: 11 am EST, ZOOM (INVITATION BY LMIG LIST SERVER) Speaker: Dr. Diego Presman (U Buenos Aires) Title: “Insights on Glucocorticoid Receptor Activity Through Live Cell Imaging” Summary: Eucaryotic transcription factors ( Read More...
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PLEASE, JOIN US AT LMIG ZOOM SEMINAR! DATE: Tuesday, January 14th, 2025 TIME AND LOCATION: 11 am EST, ZOOM (INVITATION BY LMIG LIST SERVER)Dr. Yun-Xing Wang (NIH/NCI) “Determining 3D structures of individual molecules in Read More...
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PLEASE, JOIN US AT LMIG ZOOM SEMINAR! DATE: Tuesday, January 14th, 2025 TIME AND LOCATION: 11 am EST, ZOOM (INVITATION BY LMIG LIST SERVER) Dr. Yun-Xing Wang (NIH/NCI) “Determining 3D structures of individual Read More...
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How do I determine which EM technique should be used for my research? Schedule a free consultation with the Head of EMC, Dr. Ru-ching Hsia, to discuss the aim of your research project. Dr.& Read More...