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Bioinformatics
Found in all bacteria and archaea. Also present in mtDNA and chloroplasts (See the endosymbiotic theory ). Multiple copies per genome Taxonomy based on 16S rRNA has been extremely popular, and databases are continuously growing. Classification Read More...
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Protein Expression Laboratory The Protein Expression Laboratory develops, improves, and delivers protein-centric services. Our goal is to help client investigators achieve their research goals with the lowest possible cost in the shortest time. All PEL Read More...
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Bioinformatics
The QIIME2 platform can be used for different types of -omics data. For this course, we will be focusing on targeted amplicon sequencing of the 16S rRNA gene. The 16S rRNA gene (~1500 bp) codes for Read More...
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Bioinformatics
By targeting 16S rRNA, we want to target bacteria and archaea. Therefore, we can exclude sequences that are unexpected such as those from chloroplasts or mitochondria. By setting --p-include p__ , we are retaining only sequences Read More...
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Confocal
Stephen Lockett, Ph.D. Director, OMAL locketts@nih.gov 301-846-5515 Valentin Magidson, Ph.D. Scientist magidsonv@mail.nih.gov 301-846-6092 Will Heinz, Ph.D. Scientist heinzwf@nih.gov 301-846-1239 David Read More...
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Bioinformatics
An OTU is an operational taxonomic unit. This is derived by binning sequences at a certain threshold of similarity. This threshold is generally set at 97%, which is associated with a species level assignment. Clustering can Read More...
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Bioinformatics
07/27/2023 - For 35 years, NCBI has collected a vast amount of sequence information including from a diverse array of organisms, including viruses, bacteria and fungi. With all of the databases and tools available& Read More...
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Bioinformatics
10/19/2022 - Our bodies host millions of microorganisms, and our relationship with these tiny living companions is complex. While microbial communities support normal processes and defend against harmful pathogens, infections with some viruses and bacteria can Read More...
Frederick, MD
Repositories
Trans NIH Facility
DTP’s Natural Products Repository is the world’s largest storehouse of natural products. It houses close to >200,000 extracts from samples of more than 70,000 plants and >20,000 marine organisms collected from more than 29 countries, plus extracts Read More...
Bethesda, MD
Core Facility
The Flow Cytometry Core (LGI) offers established technologies to support studies using flow cytometry and cell sorting. Established Technologies Applications that run on FACS Caliburs include: Immunophenotyping (up to 4-color), Intracellular markers, including cytokines and Read More...
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Confocal
Our Team Tatiana S. Karpova Ph.D.Core Headkarpovat@nih.govBuilding 41, Room C615240-760-6637 David A. Ball Ph.D.Core Biologistballa@nih.govBuilding 41, Room B114D240-760-6577 Mohamadreza Fazel, Ph.D.Core Biologistmohamadreza. Read More...
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Bioinformatics
Lesson 2: Getting Started with QIIME2 Lesson Objectives Obtain sequence data and sample metadata Import data and metadata Discuss other useful QIIME2 features including view QIIME2, provenance tracking, and the QIIME2 forum. DNAnexus DNAnexus provides a Read More...
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Bioinformatics
09/08/2023 - Hybrid Seminar Friday, September 8, 2023 • 9:00-10:00 a.m. Building 549 Auditorium (In-person attendance encouraged) Speaker: Brian Kelsall, M.D. Senior Investigator, Mucosal Immunobiology Section Laboratory of Molecular Immunology National Institutes of Allergy Read More...
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Bioinformatics
06/04/2021 - Speaker: Rangan Sreenivas Sukumar Distinguished Technologist Hewlett Packard Enterprise (HPE) Abstract: In March of 2020, the “Force for Good” pledge of intellectual property to fight COVID-19 brought into action HPE products, resources and expertise to Read More...
Rockville, MD
Trans NIH Facility
NISC’s role within NHGRI, and more broadly across NIH, aims to advance genome sequencing and its many applications, with a goal not simply to produce sequence data, but to produce the infrastructure required to Read More...
Bethesda, MD
Collaborative
The Pan-Microbial Serology Facility (PMSF) is part of the Center for Cancer Research (CCR) at the National Cancer Institute (NCI). The PMSF focuses on determining individualized pan-microbial immune profiles associated with human diseases including immunological Read More...
Bethesda, MD
Trans NIH Facility
The facilities at AIM are available for use by the entire NIH intramural research community. While we welcome users with any size imaging project, AIM specializes in large, yearlong (or longer), collaborative research efforts with Read More...
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Confocal
2024 Senatorov IS, Bowman J, Jansson KJ, Alilin AN, Capaldo BJ, Lake R, Riba M, Abbey YC, Mcknight C, Zhang X, Raj S, Beshiri ML, Shinn P, Ngyuyen H, Thomas CJ, Corey E, Kelly K. Castrate Read More...
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Confocal
Gianluca Pegoraro, PhD gianluca.pegoraro@nih.gov Facility Head 240-760-6696 Bldg. 41, Room B909 Dr. Pegoraro received his Ph.D. in Molecular Genetics from the International School of Superior Studies in Trieste, Italy in 2004. After Read More...
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Confocal
Gianluca Pegoraro, PhD gianluca.pegoraro@nih.gov Facility Head 240-760-6696 Bldg. 41, Room B909 Dr. Pegoraro received his Ph.D. in Molecular Genetics from the International School of Superior Studies in Trieste, Italy in 2004. After Read More...
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Bioinformatics
Lesson 4: Feature table filtering, taxonomic classification, and phylogeny Learning objectives learn how to apply different types of filtering to your ASV table and representative sequence data. classify your ASVs. Generate a phylogenetic tree. Now that Read More...
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Bioinformatics
Lesson 3: Creating a feature table Lesson Objectives Check for primers Generate an ASV count table and representative sequence file Understand the difference between OTU picking and denoising The two primary files that will be used Read More...