Web Page
Bioinformatics
07/27/2023 - This new webinar will be covering and discussing the host responses to vaccines with invited speakers Dr. Oliver He and Dr. Guanming Wu. During the hour long webinar, Vaccine Induced Gene Expression Analysis Tool ( Read More...
Web Page
Bioinformatics
Can we combine just the fold change columns in the alignment based and classification based differential analysis results to compare the two? Hint: first copy the results.csv file from the alignment based method in Read More...
Web Page
Bioinformatics
10/20/2021 - Presenter: Trevor Bedford, Ph.D. Associate Professor Vaccine and Infectious Disease Division Human Biology Division Herbold Computational Biology Program Fred Hutchinson Cancer Research Center Genomic epidemiology has enabled critical insights during the COVID-19 pandemic. Read More...
Web Page
Bioinformatics
The cut command can be used to subset tabular data by column. To do this, specify the column number using -f option and the column separator using the -d option. For instance, to subset out Read More...
Frederick, MD
Collaborative
The Biopharmaceutical Development Program (BDP) provides resources for the development of investigational biological agents. The BDP supports feasibility through development and Phase I/II cGMP manufacturing plus regulatory documentation.The BDP was established in 1993. We Read More...
Web Page
Bioinformatics
06/04/2021 - Speaker: Rangan Sreenivas Sukumar Distinguished Technologist Hewlett Packard Enterprise (HPE) Abstract: In March of 2020, the “Force for Good” pledge of intellectual property to fight COVID-19 brought into action HPE products, resources and expertise to Read More...
Web Page
Bioinformatics
From the paper where this data was obtained, the following (incomplete) list of gene markers was obtained: Mmp3: preadipocytes Mki67: proliferating cells Fabp4: differentiating beige adipocytes and differentiated beige adipocytes Scd1: differentiated beige adipocytes Ucp1: Read More...
Web Page
Bioinformatics
Let's talk about obtaining expression read counts using an application called featureCounts First let's create a new directory in our ~biostar_class/hbr_uhr folder to store the counts. mkdir hbr_uhr_deg_ Read More...
Web Page
Bioinformatics
Remember that the deseq2.r script requires that the expression counts table be in csv format. It is currently in tab delimited format as generated by featureCounts. There is also a header line that we Read More...
Web Page
Bioinformatics
Lesson 16 Practice Objectives In this lesson, we learned about the classification based approach for RNA sequencing analysis. In this approach, we are aligning our raw sequencing reads to a reference transcriptome rather than a genome. Read More...
Web Page
Bioinformatics
04/02/2021 - Speaker: Tonia Korves, Ph.D. Lead Data Scientist Data and Human-Centered Solutions Innovation Center MITRE Corporation Abstract As COVID-19 research rapidly escalated last year, we quickly built a platform to help biomedical experts track Read More...
Web Page
Bioinformatics
Go back to /data/user/hcc1395_b4b. cd /data/user/hcc1395_b4b Create a directory called hcc1395_featurecounts. mkdir hcc1395_featurecounts Go back into /data/user/hcc1395_b4b/hcc1395_hisat2 for this Read More...
Web Page
Bioinformatics
Remember that the deseq2.r script requires that the expression counts table be in csv format. It is currently in tab delimited format as generated by featureCounts. There is also a header line that we Read More...
Web Page
Bioinformatics
Let's talk about obtaining expression read counts using an application called featureCounts First let's create a new directory in our ~biostar_class/hbr_uhr folder to store the counts. mkdir hbr_uhr_deg_ Read More...
Web Page
Bioinformatics
The module featureCounts from the subread package will be used to quantify gene expression. In the featureCounts command below: -p: specifies the presence of paired end data. --countReadPairs: specifies to count both reads in a Read More...
Web Page
Bioinformatics
In this session, participants will practice generating a gene expression matrix for the HBR-UHR data. Sign onto Biowulf and change into the /data/user/hbr_uhr_b4b folder. Solution ssh user@biowulf.nih.gov Read More...
Web Page
Bioinformatics
Lesson 15 Practice Objectives Previously, we performed QC on the Golden Snidget RNA sequencing data, aligned the sequencing reads to its genome, and obtained expression counts. We can now finally perform differential expression analysis, to find Read More...
Web Page
Bioinformatics
A script allows users to string together analysis steps and keep them in one document. It can be reused with or without modification to analyze similar datasets. The script (SRR23341296_statistics.sh) below will use Read More...
Bethesda, MD
Trans NIH Facility
NIH Intramural CryoEM Consortium (NICE) serves intramural investigators in all NIH IC’s. NICE provides access to state-of-the-art Titan Krios cryo-electron microscopes for atomic-resolution structure determination of protein, macromolecular complexes, membrane receptors, cellular organelles, and Read More...
Frederick, Maryland
Core Facility
Repositories
The Biological Products Core provides the AIDS research community with high-quality purified preparations of various strains of Human Immunodeficiency Virus (HIV) and Simian Immunodeficiency Virus (SIV), economically prepared by leveraging the economy of scale. Materials Read More...
Web Page
[tabby title="Home"] About NICE-NIH Intramural CryoEM Consortium NIH Intramural CryoEM Consortium (NICE) serves intramural investigators in all NIH IC’s. NICE provides access to state-of-the-art Titan Krios cryo-electron microscopes for atomic-resolution structure determination of Read More...
Web Page
Bioinformatics
1. Introduction and Learning Objectives This tutorial has been designed to demonstrate common secondary analysis steps in a scRNA-Seq workflow. We will start with a merged Seurat Object with multiple data layers representing multiple samples that Read More...
Web Page
Bioinformatics
Lesson 15: Finding differentially expressed genes Before getting started, remember to be signed on to the DNAnexus GOLD environment. Lesson 14 review In the previous lesson, we learned to visualize RNA sequencing alignment results in the Integrative Read More...
Web Page
Bioinformatics
Note that we now have differential expression by transcripts and our first column contains the transcript IDs. But what genes do these transcripts map to? We will need to do some data wrangling to find Read More...
Web Page
Bioinformatics
Lesson 16: RNA sequencing review and classification based analysis Before getting started, remember to be signed on to the DNAnexus GOLD environment. Review In the previous classes, we learned about the steps involved in RNA sequencing Read More...