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Search Results for: K-ras exons 2-5

Total Results Found: 61

Total Results Found: 61

CLIA Molecular Diagnostics Laboratory
Frederick, Maryland

Core Facility

CLIA-Certified Technologies Offered:: Fragment Analysis for Micro-satellite Instability Detection, Pharmacoscan Array for Pharmacogenomics, Mutation Detection for PCR and Sanger Sequencing, DNA extraction from whole blood, saliva, FFPE tissues, buccal swabs, nails, hair, PBMCs, buffy coats, Read More...

October Newsletter 2022-2

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October 6, 2022 crex.nih.gov CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Science & Technology Seminars and Training Events Upcoming Seminars and Educational Opportunities The following Read More...

Introduction to Unix on Biowulf - January 2023: Question 4:

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Bioinformatics

Print the first six lines of 22_transcriptome.fa. {{Sdet}}{{Ssum}}Solution{{Esum}} head -n 6 22_transcriptome.fa >ENST00000615943.1 loc:chr22|10736171-10736283|- exons:10736171-10736283 segs:1-113 ATCACTTCTCGGCCTTTTGGCTAAGATCAACTGTAGTATCTGTTGTTATTAATATAATATTGTATATTCA ACCAATTGTCAATACAAGGCTGTTTGTATCTGATATGAACCAA >ENST00000618365.1 loc:chr22|10936023-10936161| Read More...

Data Wrangling with R: Introducing the SummarizedExperiment

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Bioinformatics

The SummarizedExperiment class and the inherited class RangedSummarizedExperiment are available in the R package SummarizedExperiment . SummarizedExperiment is a matrix-like container where rows represent features of interest (e.g. genes, transcripts, exons, etc.) and columns represent Read More...

Introduction to Unix on Biowulf - January 2023: Question 5:

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Bioinformatics

Print the last eight lines of 22_transcriptome.fa. {{Sdet}}{{Ssum}}Solution{{Esum}} tail -n 8 22_transcriptome.fa >ENST00000427528.1 loc:chr22|50798655-50799123|+ exons:50798655-50799123 segs:1-469 ATGGCACCAAAAGCGAAGGAAGCTCCTGCTCATCCTAAAGCCGAAGCCAAAGCGAAGGCTTTAAAGGCCA AGAAGGCAGTGTTGAAAGGTGTCCGCAGCCACACGCAAAAAAGAAGATCCGCATGTCACTCACCTTCAGG CGGCCCAAGACACTGCGACTCCGGAGGCAGCCCAGATATCCTCGGAAGAGCACCCCCAGGAGAAACAAGC TTGGCCACTATGCTATCATCAAGTTTCCGCTGGCCACTGAGTCGGCCGTGAAGAAGATAGAAGAAAACAA CACGCTTGTGTTCACTGTGGATGTTAAAGCCAACAAGCACCAGATCAGACAGGCTGTGAAGAAGCTCTAT GACAGTGATGTGGCCAAGGTCACCACCCTGATTTGTCCTGATAAGGAGAACAAGGCATATGTTCGACTTG CTCCTGATTATGATGCTTTCGATGTTGTAACAAAATTGGGATCACCTAA {{Edet}}

BTEP Coding Club: rMATS output format

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Bioinformatics

Output folder (specified by --od parameter) will contain files for each type of splicing event (marked as RI for "retained intron", SE for "skipped exon", A3SE for "alternative 3' splice Read More...

NCI LASP Mouse Modeling & Cryopreservation (MMC)
Frederick, MD

Core Facility

Repositories

The Mouse Modeling Core assists NIH investigators by generating and preserving genetically-engineered mouse strains. Services include scientific consultation, gene-targeting in mouse embryonic stem cells, micro-injection of nucleic acids, proteins, or ES cells into mouse embryos, Read More...

Biopharmaceutical Development Program (BDP)
Frederick, MD

Collaborative

The Biopharmaceutical Development Program (BDP) provides resources for the development of investigational biological agents. The BDP supports feasibility through development and Phase I/II cGMP manufacturing plus regulatory documentation.The BDP was established in 1993. We Read More...

Publications

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Confocal

2024 Coutinho, L. L., Femino, E. L., Gonzalez, A. L., Moffat, R. L., Heinz, W. F., Cheng, R. Y. S., Lockett, S. J., Rangel, M. C., Ridnour, L. A. & Wink, D. A. NOS2 and Read More...

Bioinformatics for Beginners 2022: Lesson 14 Practice

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Bioinformatics

Lesson 14 Practice Objectives Here, we will practice using the Integrative Genome Viewer (IGV) to visualize the hcc1395 RNA sequencing alignment results. About the data and launching IGV We were introduced to the hcc1395 RNA sequencing Read More...

Data Wrangling with R: Accessors

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Bioinformatics

As you can see from the image, there are several accessor functions to access the data from the object: assays() - access matrix-like experimental data (e.g., count data). Rows are genomic features (e.g., Read More...

Bioinformatics for Beginners 2022: Alignment

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Bioinformatics

Alignment RNASeq Mapping Challenges The majority of mRNA derived from eukaryotes is the result of splicing together discontinuous exons, and this creates specific challenges for the alignment of RNASEQ data. Mapping Challenges Reads not perfect Read More...

Bioinformatics for Beginners 2022: B4b 2022 rnaseq jw

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Bioinformatics

This page uses content directly from the Biostar Handbook by Istvan Albert. Obtain RNA-seq test data. The test data consists of two commercially available RNA samples: Universal Human Reference (UHR) and Human Brain Reference (HBR) . Read More...

BTEP Coding Club: Running rMATS on Biowulf

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Bioinformatics

The program can be run in either interactive or batch mode Get interactive node: sinteractive -c 16 --mem 45g --gres=lscratch:20 Make sure you have requested enough resources, as the program can be quite memory- and Read More...