Search Results for: SWR Brca2 exon 27 del

08/27/2024 - This 45-minute online Lunch and Learn training will help attendees develop their own customized strategy for responsibly incorporating generative artificial intelligence (AI) tools, such as ChatGPT, into their workflows.  By the end Read More...

06/27/2024 -   Alternative Meeting Information:  Meeting number: 2310 497 7985 Password: mjPjjmi$473 Join by video system Dial  23104977985@cbiit.webex.com You can also dial 173.243.2.68 and enter your meeting number. Join by phone 1-650-479-3207  Call-in number (US/Canada) Read More...

06/27/2024 - In this  in-person  session, participants will have an opportunity to discuss their own research and use of Qiagen products with Qiagen scientists. Note on Technology Participants are expected to bring Read More...

06/27/2024 - During this presentation, you will learn about the GenePattern ecosystem, an environment for accessible, reproducible research that has been serving the needs of the cancer genomics community since 2004. GenePattern hosts hundreds of genomics analysis Read More...

03/27/2024 - DAVID (the Database for Annotation, Visualization and Integrated Discovery) provides a comprehensive set of functional annotation tools for investigators to understand the biological meaning behind large lists of genes acquired from high-throughput assays such Read More...

02/27/2024 - This session will cover what are containers, and why and how to use them in your Bioinformatics workflows. We will review best practices, basic commands, and useful resources. This session is geared towards beginners.& Read More...

Which of the following will throw an error and why? 4 _ chr :1:2: unexpected input ## 1: 4_ ## ^ . 4 chr :1:3: unexpected symbol ## 1: .4chr ## ^ {{Edet}} Create the following objects; give each object an appropriate name (your best guess at what name to Read More...

12/08/2023 - This presentation will discuss strategies and policies for effective sharing and reuse of large multidimensional datasets. Dr. Espinosa will discuss his experiences as a data generator, data analyst, collaborator, teacher, and mentor through the Read More...

Now that we have downloaded the HBR and UHR dataset and know where analysis tools are, let's start learning about RNA sequencing, by first learning about our reference genome and annotation files. Let's Read More...

Now that we have downloaded the HBR and UHR dataset and know where analysis tools are, let's start learning about RNA sequencing, by first learning about our reference genome and annotation files. Let's Read More...

07/27/2023 - This course provides detailed information on managing and sharing data from the first data planning stage, through the data life cycle, to data archiving, and finally to selecting an appropriate repository for data preservation. Read More...

06/27/2023 - Participant will learn about FAIR principle (findability, acce ibility, interoperability, and reu ability) and how they apply to cientific data. Given the NIH Data haring Policy for intramural cienti t , method for organizing, managing, Read More...

04/27/2023 - Scientific journals almost always have limits on the number of figures that can be included in a publication. Don't fret, in the 6th and final lesson of the Data Visualization with R course Read More...

02/27/2023 - Data Wrangling is another class in the NIH Library Introduction to R and RStudio Series. A basic understanding of using R and RStudio to manage data is expected. This one-hour class will introduce the Read More...

01/27/2023 - This course provides detailed information on managing and sharing data from the first data planning stage, through the data life cycle, to data archiving, and finally to selecting an appropriate repository for data preservation. Read More...

10/26/2022 - The Division of Cancer Prevention is hosting the  Statistical Adjustment for Multiplicity Virtual Workshop  from  October 26-27, 2022 . Workshop Goal The goal of this workshop is to bring together medical doctors, epidemiologists, and statisticians from Read More...

09/27/2022 - This course provides detailed information on managing and sharing data from the first data planning stage, through the data life cycle, to data archiving, and finally to selecting an appropriate repository for data preservation. Read More...

09/27/2021 - The training will overview the current status of pathway tools, with focus on software available to NIH community.  It will discuss the biological interoperation of mutation and expression data in the context of pathways, Read More...

07/27/2021 - What’s the difference between “regular” statistics (i.e. what you may have been using in the past several years) and the “new” Bayesian statistics? Which one should you use for your next study? Read More...

05/27/2021 - Register here This seminar series showcases research from different Cancer Moonshot initiatives that support the 10 recommendations of the  Blue Ribbon Panel  report. These presentations will inform the community about the progress of Cancer Moonshot– Read More...

04/27/2021 - Register Session Description This course walks through using and applying two popular, freely available genome browsers and how they can help bioinformatics analysis: the University of California Santa Cruz (UCSC) Genome Browser and the Read More...

The Genomics Laboratory (formerly Laboratory of Molecular Technology) is an integrated, high-throughput molecular biology laboratory focusing on the development of genetics and genomics technologies, together with associated laboratory automation systems, data analysis, and information management Read More...

CLIA-Certified Technologies Offered: Fragment Analysis for Micro-satellite Instability Detection, Pharmacoscan Array for Pharmacogenomics, Mutation Detection for PCR and Sanger Sequencing, DNA extraction from whole blood, saliva, FFPE tissues, buccal swabs, nails, hair, PBMCs, buffy coats, Read More...

The Genomics Technology Laboratory is an integrated, high-throughput molecular biology laboratory focusing on the development of genetics and genomics technologies, data analysis, and information management tools, in support of CCR Investigators. The laboratory develops integrated Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More New CREx Program Manager The CREx Team is happy to announce Deepika Velampati as the new CREx Program Read More...

06/27/2025 - This one-hour and fifteen minute online training is part two of an introductory two-part series for those who want to learn about research data management and sharing, or for those who are interested in Read More...

05/27/2025 - Agentic AI is a class of artificial intelligence that acts autonomously to make decisions and take actions to achieve specific goals. During this event, the participants will discuss the emerging role of AI agents Read More...

03/27/2025 - Please note: Registration is required to get the Meeting Link for this event. Please pre-register. Aviv Regev is head of Genentech Research and Early Development. Formerly, Regev was Chair of the Faculty and Core Read More...

02/27/2025 - NGS produces millions of sequences per sample and the challenge is to identify where in the genome each sequence came from. Fortunately, there are aligners that help scientists with this task. After this class, Read More...

01/27/2025 - NCI CCR Liver Cancer Program Seminar Series Dr. Sethupathy is professor of physiological genomics and chair of the Department of Biomedical Sciences at Cornell University. He leads a research lab focused on genome-scale and Read More...

08/27/2024 - This hands-on workshop will help you advance your microbiome analysis and computing skills, and help you learn new ways to leverage computing resources for your research. What you’ll learn: ● The basics of interacting Read More...

Some tools have been described in the previous session (see here ). Today, we will be focusing on the SingleR tool, which also requires the celldex package . In short, SingleR operates by comparing your current dataset Read More...

One particular critique of differential expression in single cell RNASeq analysis is p-value "inflation," where the p-values get so small that there are far too many genes exist with p-values below 0.05, even after Read More...

The Seurat Object is a data container for single cell RNA-Seq and related data. It is an S4 object, which is a type of data structure that stores complex information (e.g., scRNA-Seq count matrix, Read More...

03/27/2024 - This half-day, virtual workshop features representatives from NCI cancer data cloud resources, including the  NCI Cancer Research Data Commons  (CRDC),  Institute for Systems Biology Cancer Gateway in the Read More...

03/27/2024 - On Wednesday, March 27th, at 9:00 a.m., in Building 37, Room 4041/4107, and online. In-person attendance is encouraged.  Dr. Khan is the Deputy Chief of the Genetics Branch and Head of the Oncogenomics Section, Read More...

Use pivot_longer to reshape countB. Your reshaped data should look the same as the data below. {{Sdet}} Solution } library ( tidyverse ) countB % rownames_to_column ( "Feature" ) countB_l ## 1 Tspan6 1 703 71 ## 2 Tspan6 2 567 970 ## 3 Tspan6 3 867 242 ## 4 TNMD 1 490 342 ## 5 TNMD 2 482 935 ## 6 Read More...

This is our first coding help session. We have designed some practice problems to get you acquainted with using R before beginning to wrangle in our next lesson. Practice problems Which of the following will Read More...

Load in the comma separated file "./data/countB.csv" and save to an object named gcounts . {{Sdet}} Solution } gcounts `...1` colnames ( gcounts )[ 1 ] ## 1 Tspan6 703 567 867 71 970 242 ## 2 TNMD 490 482 18 342 935 469 ## 3 DPM1 921 797 622 661 8 500 ## 4 SCYL3 335 216 222 774 979 793 ## 5 FGR 574 574 515 584 941 344 ## 6 CFH 577 792 672 104 192 936 ## 7 FUCA2 798 766 995 27 756 546 ## 8 GCLC 822 874 923 705 667 522 ## 9 NFYA 622 793 918 868 334 64 {{Edet}} Plot the Read More...

09/27/2023 - Partek Flow is your start-to-finish solution for analyzing high dimensional multi-omics sequencing data. It is a point-and-click software and is suitable for those who wish to avoid the steep learning curve associated with analyzing Read More...

While we can always download reference genomes and reference transcriptomes from repositories such as NCBI or Ensembl, we will use gffread to create one from the chromosome 22 genome (22.fa) that we have used when analyzing Read More...

Lesson 13 Practice Objectives In this lesson we learned how to align raw sequencing reads to reference and to process alignment results for downstream analysis. Here, we will test our knowledge by continuing with the Golden Read More...

Here, let's download the HBR and UHR dataset to get acquainted with it. First, we will use pwd to make sure we are in the home directory. pwd If we are in the home Read More...

Here, let's download the HBR and UHR dataset to get acquainted with it. First, we will use pwd to make sure we are in the home directory. pwd If we are in the home Read More...

Lesson 9: Reference genomes and genome annotations used in RNA sequencing Before getting started, remember to be signed on to the DNAnexus GOLD environment. Lesson 8 Review In Lesson 8, we learned about the basics of RNA sequencing, Read More...

Lesson 9: Reference genomes and genome annotations used in RNA sequencing Before getting started, remember to be signed on to the DNAnexus GOLD environment. Lesson 8 Review In Lesson 8, we learned about the basics of RNA sequencing, Read More...

BBDuk is another tool that can be used for adapter and quality trimming. In addition, BBDuk can be used to filter out contaminations, perform GC filtering, filter for length, etc. (see https://jgi.doe.gov/ Read More...

07/27/2023 - For 35 years, NCBI has collected a vast amount of sequence information including from a diverse array of organisms, including viruses, bacteria and fungi. With all of the databases and tools available& Read More...

07/27/2023 - This new webinar will be covering and discussing the host responses to vaccines with invited speakers Dr. Oliver He and Dr. Guanming Wu. During the hour long webinar, Vaccine Induced Gene Expression Analysis Tool ( Read More...

Bioconductor is an R package repository for free open-source software that "facilitates rigorous and reproducible analysis of data from current and emerging biological assays" . Bioconductor is released semi-annually, with two working Bioconductor releases Read More...

Introduction to Bioinformatics Resources at NCI (06/13, Instructor Amy Stonelake, PhD (BTEP)) In this lesson, attendees will be introduced to the different bioinformatics resources available to them at NCI. This includes: Bioinformatics Training Classes and Events Read More...

Course Overview Course Description A series of 6 stand-alone lessons introducing learners to various aspects of bioinformatics. Lessons will be held on Tuesdays at 1 PM in June and July 2023. You can attend any class, just one Read More...

06/27/2023 - In partnership with the NIH Clinical Center's Biostatistics and Clinical Epidemiology Service (BCES), the NIH Library is offering classes geared to cover general concepts behind statistics and epidemiology. This four-part lecture series will Read More...

Understanding data distribution can help us decide appropriate downstream steps in analysis such as which statistical test to use. A histogram is a good way to visualize distribution. It divides the data into bins or Read More...

Load the data For these exercises, you will explore the titanic data from kaggle.com , which was downloaded from here . You will need to download the data and load into R. As this is a Read More...

09/27/2022 - Course Objectives: This 12-week long course is for scientists wanting to learn basic bioinformatics skills. Material will be taught at a beginner to intermediate level. There are no pre-requisites. Learners will: Learn Unix skills Read More...

09/27/2022 - Speaker: Neil L. Kelleher, Ph.D. Walter and Mary E. Glass Professor of Molecular Biosciences Professor of Chemistry in the Weinberg College of Arts and Sciences Professor of Medicine (Hematology & Oncology) in Read More...

07/27/2022 - This July, the CGC Webinar Series features a talk by Dr. Nadia Lanman from Purdue University. In this upcoming webinar, Dr. Lanman will present her research studying human gliomas using dog as a model Read More...

07/27/2022 - Many recent studies highlighted the improved capability of long-read sequencing to detect structural variation in the human genome. For example, these technologies was also recently utilized to produce the first complete assembly of the Read More...

06/27/2022 - This is the first class in the NIH Library Introduction to R Series. This class provides a basic overview of the functionality of R programming language and RStudio. R is a programming language and Read More...

04/27/2022 - In partnership with NCI’s Cancer Moonshot SM    Initiative, the Society for Immunotherapy of Cancer (SITC) and the  Big Data  and  Data Sharing  Committee are hosting the second of nine webinars that make up Read More...

01/27/2022 - In partnership with the NIH Clinical Center's Biostatistics and Clinical Epidemiology Service (BCES), the NIH Library is offering a class geared to cover the general concepts behind common statistical tests. This two-part lecture Read More...

10/27/2021 - Wrapping up its final webinar of 2021, the NCI Cancer Research Data Commons (CRDC) Cancer Genomics Cloud (CGC) welcomes Cold Spring Harbor Laboratory fellow Dr. Pascal Belleau. He will share his findings from recent work Read More...

09/27/2021 - The NCI Genomic Data Commons’ (GDC) September webinar will introduce cancer researchers and bioinformaticians to the GDC's single-cell RNA-Seq (scRNA-Seq) data workflow. Single cell sequencing is a powerful platform for studying tumor heterogeneity Read More...

05/27/2021 - Register Now Faculty:   Shannon McWeeney, PhD – Oregon Health & Science University; NCI Cancer Moonshot DRSN Moderator:   Santosh Putta, PhD Target Audience This series will serve as an excellent resource for all stakeholders interested Read More...

05/27/2021 - RNA sequencing (RNA-seq) is a widely used method in genomics, which enables the interrogation of whole cellular transcriptomes. The CCR Collaborative Bioinformatics Resource (CCBR) has implemented a RNA-seq workflow on the NIDAP collaboration platform, Read More...

04/27/2021 - Registration is required. Register at this link . Python is a good programming language for scientists to solve a wide variety of biological problems. We find that many scientists who come to Software Carpentry workshops Read More...

01/27/2021 - Please plan to attend the Earl Stadtman Investigator Program search seminar by: Qian Zhu, Ph.D. Dana Farber Cancer Institute/Boston Children's Hospital Dr. Zhu's research interests include: single-cell genomics; spatial transcriptomics; Read More...

01/27/2021 - Register now and join us via Webex. Speakers: Esmeralda Casas-Silva, Ph.D.; Veena Gopalakrishnan, Ph.D.; Helen Moore, Ph.D.; Claire Blaustein National Cancer Institute NCI's Cancer Moonshot℠ Biobank will engage diverse participants Read More...

08/21/2020 - Abstract: Machine learning (ML) has emerged as an essential tool for building models which can be used to predict clinical outcomes for age-related diseases. A significant challenge of ML is knowing which algorithms and Read More...

07/27/2020 - The GDC RNA-Seq Analysis pipeline quantifies protein-coding gene expression. RNA-Seq data is aligned to the reference genome to detect splice junctions and then re-aligned to increase quality. Gene expression quantification and fusion detection are Read More...

02/27/2020 - The Annotation, Visualization and Impact Analysis (AVIA) is an application developed to guide, prioritize and summarize genomic variants. AVIA maintains and aggregates dozens of publicly available variant annotation databases and predictions from impact analysis Read More...

02/27/2020 - In this symposium, distinguished scientists will share recent successes in their research to visualize RNA targets at the single molecule level with single cell resolution and morphological context. Here are some of the exciting Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More NIH Cores on CREx NIBIB BEPS Micro Analytical Immunochemistry Unit The Micro Analytical Immunochemistry Unit employs a variety Read More...

CREx News & Updates January 2022 Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Site Spotlight NCI ANTIBODY CHARACTERIZATION LABORATORY (ACL) The ACL specializes in rigorously validating antibodies for signaling and Read More...

The OSTR offers cutting-edge technology platforms to the CCR scientific community through centralized facilities. The videos accessed through this page are designed to introduce the various scientific methodologies OSTR makes available through the cores on Read More...

Most BTEP courses include detailed course materials including lesson content, additional resources, and lesson associated data. These course materials are listed here so that learners can easily return to and review concepts taught in class Read More...

Listed below are the video recordings of past BTEP events (classes, seminars, workshops). Videos are hosted on various servers and may play slightly differently. Some videos may be downloaded for local viewing. Recorded Videos of Read More...

1. Introduction and Learning Objectives This tutorial has been designed to demonstrate common secondary analysis steps in a scRNA-Seq workflow. We will start with a merged Seurat Object with multiple data layers representing multiple samples that Read More...

This lesson provides an introduction to R in the context of single cell RNA-Seq analysis with Seurat. Learning Objectives Learn about options for analyzing your scRNA-Seq data. Learn about resources for learning R programming. Learn Read More...

Help Session Lesson 4 Plotting with ggplot2 For the following plots, let's use the diamonds data ( ?diamonds ). The diamonds dataset comes in ggplot2 and contains information about ~54,000 diamonds, including the price, carat, color, clarity, and Read More...

Help Session Lesson 3 Loading data Import data from the sheet "iris_data_long" from the excel workbook (file_path = "./data/iris_data.xlsx"). Make sure the column names are unique and Read More...

The SRA (Sequence Read Archive) at NCBI is a large, public database of DNA sequencing data. The repository holds "short reads" generated by high-throughput next-generation sequencing, usually less than 1,000 bp. We will download Read More...

Let's now take a look at our final differential analysis results table (results_with_gene_names_labeled.txt), using the SLC2A11 gene as an example and below we use the column command to Read More...

fastq-dump and fasterq-dump can be used to download FASTQ-formatted data. Both download the data in SRA format and convert it to FASTQ format. fastq-dump SRR1553607 creates the file: SRR1553607.fastq Check the file to make Read More...

fastq-dump and fasterq-dump can be used to download FASTQ-formatted data. Both download the data in SRA format and convert it to FASTQ format. fastq-dump SRR1553607 creates the file: SRR1553607.fastq Check the file to make Read More...

Note that we now have differential expression by transcripts and our first column contains the transcript IDs. But what genes do these transcripts map to? We will need to do some data wrangling to find Read More...

Here, let's change back in the ~/biostar_class/hbr_uhr/hbr_uhr_hisat2 folder. cd $hbr_uhr_hisat2 To align FASTQ files for one sample, we construct the HISAT2 command with the following options Read More...

Lesson 16: RNA sequencing review and classification based analysis Before getting started, remember to be signed on to the DNAnexus GOLD environment. Review In the previous classes, we learned about the steps involved in RNA sequencing Read More...

To align FASTQ files for one sample, we construct the HISAT2 command with the following options. The "-x" flag prompts us to enter the base name (ie. without extension) of genome index. The Read More...

This page uses content directly from the Biostar Handbook by Istvan Albert. Review: * downloading data from SRA * decompressing tar files * e-utilities * fastq-dump Learn: * sra-stat * XML format * automating SRA downloads * working with comma-separated values (csv) format * Read More...

Lesson 6: sra-tools, e-utilities, and parallel This page uses some content directly from the Biostar Handbook by Istvan Albert. Lesson 5 Review: The majority of computational tasks on Biowulf should be submitted as jobs: sbatch or swarm Read More...

Lesson 6: Downloading data from the SRA For this lesson, you will need to login to the GOLD environment on DNAnexus. Lesson 5 Review: The majority of computational tasks on Biowulf should be submitted as jobs: sbatch Read More...

Lesson 11: Merging FASTQ quality reports and data cleanup Before getting started, remember to be signed on to the DNAnexus GOLD environment. Lesson 10 Review In the previous lesson, we learned about the structure of the FASTQ Read More...

Lesson 13: Aligning raw sequences to reference genome Before getting started, remember to be signed on to the DNAnexus GOLD environment. Lesson 11 Review In Lesson 11 we learned to aggregate multiple FASTQC reports into one using MultiQC, Read More...

Lesson 13: Aligning raw sequences to reference genome Before getting started, remember to be signed on to the DNAnexus GOLD environment. Lesson 11 Review In Lesson 11 we learned to aggregate multiple FASTQC reports into one using MultiQC, Read More...

Lesson 1: Introduction to Biowulf, Unix, and R Learning Objectives Learn about why you may want to use R on Biowulf. Refresh Unix and R skills. This lesson will not be hands on. Why use R Read More...

Stat Transformations: Bar plots, box plots, and histograms Objectives Review the grammar of graphics template Learn about the statistical transformations inherent to geoms Review data types Create bar plots, box & whisker plots, and histograms. Read More...