Collaborative

Antibody Characterization Laboratory (ACL)
Frederick, MD

The Antibody Characterization Laboratory (ACL) is the laboratory responsible for the development of well-characterized monoclonal antibody reagents. The NCI’s Office of Cancer Clinical Proteomics Research funds ACL as a resource to the entire cancer research community. ACL produces monoclonal antibodies to putative cancer targets selected by an Antibody Scientific Committee, and each antibody is characterized using optimized Standard Operating Procedures (SOPs). Approximately once a year there is a reagent target request open to the extramural research community. ACL is operated by Leid… [learn more]

colantos@mail.nih.gov: colantos@mail.nih.gov
Email: colantos@mail.nih.gov
Phone: (301) 846-7309

Collaborative

Antibody Engineering Program
Bethesda, MD
Services Offered
Training Opportunities

The Antibody Engineering Program (AEP) is part of the Center for Cancer Research (CCR) at the National Cancer Institute (NCI). The program focuses on generating therapeutic antibodies by phage display technology, including single domain antibodies (also known as ‘nanobodies’). While antibody-based therapeutics have emerged as a major component in clinical applications, the generation of antibodies to important targets such as cell surface receptors, ion channels, and glycoproteins remains challenging. Single domain antibodies have shown a promising ability to target difficult antigen… [learn more]

Senior Investigator: Mitchell Ho, PhD
Email: homi@mail.nih.gov
Phone: (240) 760-7848

Collaborative

Chemical Biology Laboratory
Frederick, Maryland
Services Offered
Training Opportunities

Bruker AVANCE 400 and 500 MHz NMR instruments. Helium Cryoprobe technology on the 500 MHz machine for added sensitivity, especially for Carbon-13 spectra. Access to a second 500 MHz instrument with Prodigy  Liquid Nitrogen-cooled cryoprobe . User Accounts can be created for automated setup and experiment acquisition.  Use of ICONNMR automated software with 24-slot sample changers on each machine. User training available. Some service to those who are unfamiliar with NMR can be performed either as a one-time aid for compound identification or as a collaborative project for more extensive … [learn more]

Senior Scientist: Joseph J. Barchi Jr.
Email: barchij@mail.nih.gov
Phone: (301) 846-5905

Collaborative

NCI Center for Structural Biology: Protein Technology Core
Frederick, MD
Services Offered

The primary objective of the Protein Technology Core (PTC) is to establish an experimental screening pipeline for single-domain antibodies termed nanobodies. Nanobodies are antibody fragments consisting of a single monomeric variable antibody domain. The PTC, which has recently undergone renovation, is equipped with state-of-the-art equipment to enable efficient screening of synthetic libraries utilizing ribosome display technologies and those derived from the immunization of alpacas. Enrichment of selection can be easily monitored by qPCR using QuantStudio5 for synthetic and immunogenic nano… [learn more]

Head: Anna Valkov
Email: Anna.valkov@nih.gov
Phone: (301) 846-1519

Core

Collaborative

NCI Collaborative Protein Technology Resource - Mass Spectrometry
Bethesda, MD
Services Offered
Training Opportunities

The Mass Spectrometry Unit of the Collaborative Protein Technology Resource uses mass spectrometry for the study of proteins. We perform collaborative experiments with CCR researchers in areas such as: interactomes, quantitative global proteome analyses, and identification of sites of post-translational modification. In addition, we perform structural proteomics analyses. Finally, we also have an ICP-MS for analysis of inorganic atoms. Contact Details Manager: Lisa Jenkins, PhD Phone: 240.760.7209 Email: lisa.jenkins@nih.gov Instrumentation Thermo Explor… [learn more]

Senior Associate Scientist: Lisa Jenkins
Email: jenkinsl@mail.nih.gov
Phone: (240) 760-7209

Core

Collaborative

NCI Collaborative Protein Technology Resource - Nanoscale Protein Analysis
Bethesda, MD
Services Offered
Training Opportunities

The Nanoscale Protein Analysis Section of the CCR Collaborative Protein Technology Resource (CPTR) provides expertise and service in state-of-the-art protein analysis technologies to advance CCR research in basic discovery and translation/clinical studies. Applications include: Quantitative profiling of cell signaling and post-translational modification Measurement of cytokine, metabolite and serum/plasma markers Identification and validation of biomarkers & therapeutic targets Assessment of on‐ and off‐target drug activity and pharmacodynamic evaluation Study of tumor microenviron… [learn more]

Facility Head: Noemi Kedei, MD
Email: kedein@nih.gov
Phone: (240) 760-6804

Core

NCI Protein Characterization Laboratory (PCL)
Frederick, MD

DescriptionProtein Characterization Laboratory (PCL) offers various technologies to CCR investigators to characterize proteins and metabolites. The laboratory develops and applies state-of-the-art analytical technologies, primarily mass spectrometry, liquid chromatography, and Surface Plasmon Resonance (SPR), to advance the understanding of cellular function at the protein, proteomics, metabolite and metabolomic levels.PCL engages in both short and long-term collaborations based on the need of each project.  PCL is operated by Leidos Biomedical Research Inc. on behalf of NCI as part of … [learn more]

andressont@mail.nih.gov: andressont@mail.nih.gov
Email: andressont@mail.nih.gov
Phone: (301) 846-7190

Trans NIH

NIH Center for Human Immunology, Inflammation, and Autoimmunity (CHI)
Bethesda, MD
Services Offered

The NIH Center for Human Immunology, Inflammation, and Autoimmunity (CHI) is a Trans-NIH initiative whose mission is to provide a collaborative resource to enable high-dimensional multiplex immune phenotyping for NIH clinical studies. This is accomplished through a focused team for scientific input, advanced technologies, and informatics support to better understand human immune function and pathophysiology.Established TechnologiesSequencing based technologies – CITE-seq and ASAP-seq to profile both hundreds of proteins and the transcriptome or chromatin accessibility in the same single cel… [learn more]

Communications Manager: Rachel Tracy
Email: richard.apps@nih.gov
Phone: (301) 761-7429

Core

The Protein Expression Laboratory
Frederick, Maryland
Services Offered

The Protein Expression Laboratory produces proteins to help CCR investigators achieve their research goals with the lowest possible cost in the shortest time. PEL is operated by Leidos Biomedical Research Inc. on behalf of NCI as part of the Frederick National Laboratory. Contact Details Laboratory Director: Dom Esposito, PhD Phone: 301-846-7376 Email: espositod@mail.nih.gov Address: ATRF, 8560 Progress Drive, Frederick, MD, 21701 Group Leader, Protein Production: Jane Jones Phone: 301-846-1201 Email: jonesj4@mail.nih.gov Address: ATRF, 8560 Progress Drive, F… [learn more]

Director: Dom Esposito
Email: espositod@mail.nih.gov
Phone: (301) 846-1201