Genetics and Genomics

The CCR Genomics Core is located in Building 41 on the NIH Bethesda campus. The primary goal of the Core is to provide investigators from CCR/NCI and other NIH Institutes access to genomic technologies and Next-Generation Sequencing (NGS) with rapid turnaround on smaller-scale projects or projects that are not ready for production. The Core is unique in granting user-accessible instrumentation. Additional resources include training, consultation services, bioinformatics support, and secure data delivery/management. We also provide DNA and RNA quality control (QC) services. The facility operate… [learn more]

The Genomics Technology Laboratory is an integrated, high-throughput molecular biology laboratory focusing on the development of genetics and genomics technologies, data analysis, and information management tools, in support of CCR Investigators. The laboratory develops integrated strategies using multiple technology platforms that maximize the value of research or clinical samples and lead to the identification of pathways, genes, or gene products involved in the development of disease, as well as the assembly of genetic or expression profiles for the identification of prognostic and diagnost… [learn more]

The introduction of DNA sequencing instruments capable of producing millions of DNA sequence reads in a single run has profoundly altered the landscape of genetics and cancer biology. Complex questions can now be answered at previously unthinkable speeds and a fraction of their former cost. At the Sequencing Facility, researchers are provided access to the latest technologies, with consultation and Q&A services available throughout the design and execution of sequencing projects. SF is a CCR-dedicated facility open to all CCR Investigators. Dedicated capacity has also been established for NAID… [learn more]

The rapid advancement of single-cell technology has provided new powerful tools to answer many biological questions, such as identifying new or rare cell populations and characterizing the complexities of tumor heterogeneity.  Realizing the great potential of single-cell technology in cancer research, the CCR has established the Single Cell Analysis Facility (SCAF) located on the Bethesda main campus.The SCAF works closely with CCR Sequencing Facility to also extend support to CCR Investigators located in Frederick, and works with other associated support groups at CCR, including CCR Genomic… [learn more]

The Functional Genomics Laboratory (formerly, the RNAi Screening Facility) of the National Center for Advancing Translational Sciences (NCATS) assist investigators with all stages of project planning and execution, beginning with assay development through genome-wide siRNA screens, informatics/pathway analysis and rigorous follow-up. Genome-wide siRNA screens for human and mice are available. Also routinely included in screens are miRNA mimic and inhibitor libraries. Resources: Screening Libraries Ambion Silencer Select Human Genome-Wide siRNA library targeting ~22,000 genes with th… [learn more]

The Cancer and Inflammation Program – Microbiome and Genetics Core (CIP-MGC) grew out of the former CIP Genetics Core to meet the increasing need for sequencing and analysis of commensal microbiota within CIP and NCI. The MGC team specializes in: - sample handling, purification and 16S sequencing - bioinformatic processing and computational analysis of sequenced reads - support in genetics and statistical epidemiology Established TechnologiesWe use the Eppendorf epmotion 5073 and 5075 robots for the normalization of sample concentrations and sequencing library preparation and one llu… [learn more]

NCI LASP Genome Modification Core (GMC) is a CCR-dedicated facility that provides advice, training, and reagents to NCI scientists seeking to utilize CRISPR and other nucleases to generate genome modifications in primary cells, cell lines, and in animal models. Established Technologies : We are currently offering a variety of services to CCR investigators. Moreover, we also have accumulated many different flavors of genome editing reagents. Here is a brief summary of what we are currently offering: Consulting – if you are new to these areas of research and wish to find out more … [learn more]

Molecular Cytogenetics Core Facility facilitates the assessment of structural and numerical genomic changes in pre-cancer and cancer research models. This core provides comprehensive support for the cytogenetic analysis of cells from human and research animal sources. A wide range of chromosome analysis techniques are available; ranging from basic G-banded karyotyping, to 24-color Spectral Karyotyping (SKY), as well as Fluorescence in situ Hybridization (FISH) mapping of cloned DNAs. Services include:: Karyotype characterization of new cell lines; human, mouse, and rat, Confirmat… [learn more]