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Bioinformatics
Now that authentication is setup, we can return to the remote. This command will push the changes from our local repository to the repository on GitHub: git push origin main If you set up a Read More...
Frederick, MD
Core Facility
Repositories
The Mouse Modeling Core assists NIH investigators by generating and preserving genetically-engineered mouse strains. Services include scientific consultation, gene-targeting in mouse embryonic stem cells, micro-injection of nucleic acids, proteins, or ES cells into mouse embryos, Read More...
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Bioinformatics
When using SingleR, the 3 primary parameters are the experimental dataset, the reference dataset, and the labels being used. Continuing with the main labels of the MouseRNASeq dataset on the full dataset looks like this: annot = Read More...
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Bioinformatics
Suppose that a colleague added the following line to this_is_a_r_script.R and posted to a new branch called test_branch1 on the GitHub repository for further testing and development. library(pheatmap) Read More...
Bethesda, MD
Core Facility
The core provides access to several different state-of-the-art 3D microscopes as well as computers to visualize and process image data. The facility houses equipment for 2D or 3D imaging of fixed and living specimens. High Read More...
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Bioinformatics
06/24/2020 - Join us for a webinar: How to Analyze Single Cell RNA-Seq Data: Point, Click, Done Register: https://www.partek.com/webinar/how-to-analyze-single-cell-rna-seq-data-point-click-done/ Single cell mRNA sequencing allows for the identification of different cell subtypes Read More...
Frederick, MD
Collaborative
The Biopharmaceutical Development Program (BDP) provides resources for the development of investigational biological agents. The BDP supports feasibility through development and Phase I/II cGMP manufacturing plus regulatory documentation.The BDP was established in 1993. We Read More...
Frederick, Maryland
Core Facility
CLIA-Certified Technologies Offered: Fragment Analysis for Micro-satellite Instability Detection, Pharmacoscan Array for Pharmacogenomics, Mutation Detection for PCR and Sanger Sequencing, DNA extraction from whole blood, saliva, FFPE tissues, buccal swabs, nails, hair, PBMCs, buffy coats, Read More...
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Bioinformatics
Much like pseudobulk differential expression, the RNA expression can be collapsed into pre-defined components, such as the clusters, if it is believed that cell-to-cell variation is inducing too much confusion in the labeling. This collapsing Read More...
Web Page
Bioinformatics
Changes to a coding project (including scripts, data, and other content) should be saved periodically, similar to clicking on the "save" button to periodically save changes when constructing a word document. This is Read More...
Web Page
Bioinformatics
1. Introduction and Learning Objectives This tutorial has been designed to demonstrate common secondary analysis steps in a scRNA-Seq workflow. We will start with a merged Seurat Object with multiple data layers representing multiple samples that Read More...