Search Results for: miRNA inhibitor libraries

library(pheatmap) ## for heatmap generation library(tidyverse) ## for data wrangling library(ggplotify) ## to convert pheatmap to ggplot2 library(heatmaply) ## for constructing interactive heatmap

library(ggplot2) library(pheatmap) library(tidyverse) library(ggplotify) library(heatmaply)

library(pheatmap) ## for heatmap generation library(tidyverse) ## for data wrangling library(ggplotify) ## to convert pheatmap to ggplot2 library(heatmaply) ## for constructing interactive heatmap

05/17/2022 - Python is one of the preferred programming languages for scientists to solve a wide variety of biological problems. We find that many scientists who come to Software Carpentry workshops use Python and want to Read More...

04/27/2021 - Registration is required. Register at this link . Python is a good programming language for scientists to solve a wide variety of biological problems. We find that many scientists who come to Software Carpentry workshops Read More...

11/07/2024 - In the era of renewed space exploration, understanding female health risks during spaceflight is essential. This talk focuses on leveraging NIAID's ImmPort and NASA's GeneLab data using computational and systems biology to Read More...

libraries syntax variables functions data types loops and conditionals

libraries syntax variables functions data types (dictionaries and tuples in python) loops and conditionals

06/15/2020 - Participants will learn about data loading, quality control, statistical analysis as well as biological contextualization of miRNA microarray data.

10/16/2024 - In this webinar, attendees will learn to call MATLAB from Python and to call Python libraries from MATLAB.  In addition, they will learn how to use MATLAB’s Python integration to improve the compatibility Read More...

The test data consists of two commercially available RNA samples: Universal Human Reference (UHR) and Human Brain Reference (HBR) . The UHR is total RNA isolated from a diverse set of 10 cancer cell lines. The HBR Read More...

The test data consists of two commercially available RNA samples: Universal Human Reference (UHR) and Human Brain Reference (HBR) . The UHR is total RNA isolated from a diverse set of 10 cancer cell lines. The HBR Read More...

This page uses content directly from the Biostar Handbook by Istvan Albert. Obtain RNA-seq test data. The test data consists of two commercially available RNA samples: Universal Human Reference (UHR) and Human Brain Reference (HBR) . Read More...

This page uses content directly from the Biostar Handbook by Istvan Albert. Obtain RNA-seq test data. The test data consists of two commercially available RNA samples: Universal Human Reference (UHR) and Human Brain Reference (HBR) . Read More...

The test data consists of two commercially available RNA samples: Universal Human Reference (UHR) and Human Brain Reference (HBR) . The UHR is total RNA isolated from a diverse set of 10 cancer cell lines. The HBR Read More...

RNA-Seq (RNA sequencing), uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA in a biological sample at a given moment. (Wikipedia) Strictly speaking this could be any type of RNA (mRNA, rRNA, Read More...

Pathway Analysis IPA (Qiagen - CCR License) Future talk Functional Analysis Gene Set Enrichment Analysis (GSEA) https://www.gsea-msigdb.org/gsea/index.jsp DAVID https://david.ncifcrf.gov/ Enrichr https://maayanlab.cloud/Enrichr/ Other Types Read More...

In Lesson 2, partipants will learn about ways to use R on Biowulf. The focus will be on interactively working with R on Biowulf. Two different ways of accessing RStudio will be demonstrated. In addition, there Read More...

A programming language is a formal language that specifies a set of instructions for a computer to perform specific tasks. It is used to write software programs and applications, and to control and manipulate computer Read More...

07/13/2023 - In Lesson 2 of Toward Reproducibility with R on Biowulf , partipants will learn about ways to use R on Biowulf. The focus will be on interactively working with R on Biowulf. Two different ways of Read More...

To begin plotting, we need to load our ggplot2 library. Package libraries must be loaded every time you open and use R. If you haven't yet installed the ggplot2 package on your local machine, Read More...

06/16/2022 - For the Advanced Training, topics covered depend upon survey results: find key hubs using over-connectivity analysis; using Microarray repository for gene comparisons against public data; building networks with MetaCore; constructing your own pathway maps; Read More...

10/19/2021 - For the Advanced Training, topics covered depend upon survey results: find key hubs using over-connectivity analysis; using Microarray repository for gene comparisons against public data; building networks with MetaCore; constructing your own pathway maps; Read More...

05/19/2021 - Register Kinase inhibitors have been intensively studied and used effectively for cancer treatment for decades. Yet, despite our progress in understanding kinases in oncology, more needs to be known to better predict how and Read More...

05/19/2021 - Register/Join Kinase inhibitors have been intensively studied and used effectively for cancer treatment for decades. Yet, despite our progress in understanding kinases in oncology, more needs to be known to better predict how Read More...

The primary objective of the Protein Technology Core (PTC) is to establish an experimental screening pipeline for single-domain antibodies termed nanobodies. Nanobodies are antibody fragments consisting of a single monomeric variable antibody domain. The PTC, Read More...

NCI LASP Genome Modification Core (GMC) is a CCR-dedicated facility that provides advice, training, and reagents to NCI scientists seeking to utilize CRISPR and other nucleases to generate genome modifications in primary cells, cell lines, Read More...

The Antibody Engineering Program (AEP) is located at the Laboratory of Molecular Biology, which is part of the Center for Cancer Research (CCR), an intramural program at the National Cancer Institute (NCI). AEP focuses on Read More...

The Molecular Targets Program (MTP) is an organizational entity within the Center for Cancer Research (CCR) at NCI. The MTP provides the focus and infrastructure to enable CCR tenured and tenure-track Principal Investigators to initiate Read More...

To begin plotting, we need to load our ggplot2 library. Package libraries must be loaded every time you open and use R. If you haven't yet installed the ggplot2 package on your local machine, Read More...

10/23/2023 - QIAGEN Ingenuity Pathway Analysis (IPA) is the leading pathway analysis application among the life science research community and is cited in tens of thousands of articles for the analysis, integration and interpretation of data Read More...

10/11/2023 - This session focuses on R data types and data structures. In R, more advanced libraries (code) may require data to be a particular data type or data structure to perform a function or analysis. Read More...

Trim and/or filter sequence to remove sequencing primers/adaptor and poor quality reads. Example programs: FASTX-Toolkit is a collection of command line tools for Short-Reads FASTA/ FASTQ files preprocessing.
 SeqKit is an ultrafast comprehensive Read More...

developed as early as 1991 high-level, popular, general-purpose programming language that has a readable and easy to learn syntax Key features: easy to read easy to learn interpreted multi-platform wide community open source libraries (> 300,000) Two Read More...

developed as early as 1991 high-level, popular, general-purpose programming language that has a readable and easy to learn syntax Key features: easy to read easy to learn interpreted multi-platform wide community open source libraries (> 300,000) Two Read More...

A programming language is a formal language that specifies a set of instructions for a computer to perform specific tasks. It's used to write software programs and applications, and to control and manipulate computer Read More...

07/13/2023 - MATLAB provides two-way integration with many programming languages such as Python, which allows for greater collaboration between investigators. This webinar will cover how to call MATLAB from Python and how to call Python libraries Read More...

06/22/2023 - Scientists and educators from many different fields need to find and understand chemical data to perform their work. As such, understanding PubChem, the world’s largest collection of freely accessible chemical information, Read More...

ggplot2 is a R graphics package from the tidyverse collection. It allows the user to create informative plots quickly by using a 'grammar of graphics' implementation, which is described as "a coherent system for Read More...

04/06/2022 - Life Rafts in a Sea of Data: The Role of Librarians in Supporting Data Sharing Abstract:  The NIH has updated its policies on managing and sharing research data and will require a Data Management Read More...

01/19/2022 - For our next CDSL Webinar we will have a guest lecture by  Dr. Russell Rockne from Beckman Research Institute, City of Hope National Medical Center. Abstract: Temporal dynamics of gene expression inform cellular and Read More...

10/12/2021 - MATLAB provides two-way integration with many programming languages such as Python, which allows for greater collaboration between investigators. This webinar will cover how to call MATLAB from Python and how to call Python libraries Read More...

04/28/2021 - Register/Join This month’s Cancer Genomics Cloud (CGC) webinar welcomes two bioinformaticians, Dr. Vesna Pajic and Nevena Vukojicic, to show attendees how CGC features can be used to perform multi-omics analysis. Dr. Pajic Read More...

09/24/2020 - For the Advanced Training, topics covered depend upon survey results: find key hubs using over-connectivity analysis; using Microarray repository for gene comparisons against public data; building networks with MetaCore; constructing your own pathway maps; Read More...

The CCR Genomics Core is located in Building 41 on the NIH Bethesda campus. The primary goal of the Core is to provide investigators from CCR/NCI and other NIH Institutes access to genomic technologies and Read More...

The Genomics Technology Laboratory is an integrated, high-throughput molecular biology laboratory focusing on the development of genetics and genomics technologies, data analysis, and information management tools, in support of CCR Investigators. The laboratory develops integrated Read More...

The NCI Genomic Data Commons (GDC) was established by the NCI Center for Cancer Genomics (CCG) to support the receipt, harmonization, distribution, and analysis of genomic and clinical data from cancer research programs. The GDC Read More...

The CCR Collaborative Bioinformatics Resource (CCBR) is a centrally funded resource group which provides a mechanism for CCR researchers to obtain many different types of bioinformatics assistance to further their research goals. The group has Read More...

The Antibody Engineering Program (AEP) is part of the Center for Cancer Research (CCR) at the National Cancer Institute (NCI). The program focuses on generating therapeutic antibodies by phage display technology, including human antibodies and Read More...

NISC’s role within NHGRI, and more broadly across NIH, aims to advance genome sequencing and its many applications, with a goal not simply to produce sequence data, but to produce the infrastructure required to Read More...

We are a bioinformatics team within the Center for Biomedical Informatics and Information Technology’s (CBIIT’s) Cancer Informatics Branch (CIB)—soon to be referred to as the Informatics and Data Science (IDS) Program. Headed Read More...

DTP’s Natural Products Repository is the world’s largest storehouse of natural products. It houses close to >200,000 extracts from samples of more than 70,000 plants and >20,000 marine organisms collected from more than 29 countries, plus extracts Read More...

Protein Expression Laboratory The Protein Expression Laboratory develops, improves, and delivers protein-centric services. Our goal is to help client investigators achieve their research goals with the lowest possible cost in the shortest time. All PEL Read More...

RNA-SEQ Overview What is RNASEQ ? RNA-Seq (RNA sequencing), uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA in a biological sample at a given moment. (Wikipedia) Strictly speaking this could be any Read More...

10/23/2024 - Please send any questions and/or specific topic areas that you’re interested in hearing about for this presentation by Friday, 10/18 to Kayla Strauss . QIAGEN Ingenuity Pathway Analysis (IPA) is the leading Read More...

04/21/2024 - Looking for an introduction to artificial intelligence (AI) or an opportunity to expand on what you already know? Attend this workshop hosted by the NIH-funded Bridge2AI CHoRUS network and the University of Florida Read More...

ggplot2 is an R graphics package from the tidyverse collection. It allows the user to create informative plots quickly by using a 'grammar of graphics' implementation, which is described as "a coherent system for Read More...

Factor in at least 3 replicates (absolute minimum), but 4 if possible (optimum minimum). Biological replicates are recommended rather than technical replicates. Always process your RNA extractions at the same time. Extractions done at different times lead Read More...

Resources Tertiary Analysis - Biological Meaning Pathway Analysis IPA (Qiagen - CCR License) Future talk Functional Analysis Gene Set Enrichment Analysis (GSEA) https://www.gsea-msigdb.org/gsea/index.jsp DAVID https://david.ncifcrf.gov/ Enrichr Read More...

Data Analysis Here are a pair of examples of RNASEQ complete workflows RNASEQ Pipeline from NCI CCBR https://github.com/CCBR/Pipeliner/blob/master/RNASeqDocumentation.pdf RNASEQ Nextflow Pipeline from nf-core https://nf-co.re/rnaseq Read More...

Can you speed up your code with parallelization? Considerations: levels of parallelization: multiprocessing vs multithreads The most common form of parallelism in R is multiprocessing. This is usually explicitly done by you or package you Read More...

Course Overview Welcome to Toward Reproducibility with R on Biowulf This course includes a series of four lessons designed for beginner to intermediate R users interested in working with R on Biowulf. The purpose of Read More...

Once we have done this, we can activate renv within our project. But, first, let's verify the location of our renv cache. R renv::paths$cache() # Check the cache location renv::init(bioconductor = "3.16& Read More...

Learning Objectives Learn about popular programming languagues in bioinformatics Compare advantages and disadvantages of Python and R Discuss what you will need to learn to use these languages Discuss learning resources Choosing a programming language Read More...

Learning Objectives Learn about popular programming languagues in bioinformatics Compare advantages and disadvantages of Python and R Discuss what you will need to learn to use these languages Discuss learning resources Choosing a programming language Read More...

A common question posed to the Bioinformatics Training and Education Program (BTEP) is “How can I learn R and Python to analyze my data?”. First, it’s important to state that learning any programming language Read More...

High resolution single cell profiling assays have provided an unprecedented view of many biological systems and processes, but the spatial context in which this biology is occurring is often crucial. Spatial profiling, including spatial transcriptomic Read More...

In this lesson we will use two different sets of data. First, we will use data available with your base R installation, the iris data set, which is stored in object iris . These data include Read More...

12/09/2022 - PhysioNet is a data sharing platform that began as an outreach component for an NIH research project in 1999. Rebuilt in 2019 following FAIR principles (Findable, Accessible, Interoperable, Reusable), the platform has grown rapidly. It now Read More...

11/15/2022 - This workshop, geared toward researchers and information professionals, will provide an overview of appraising and preparing research data for the purpose of data sharing through the Data Curation Network’s CURATE(D) model. During Read More...

11/01/2022 - Learn how to get started on using QIAGEN’s Ingenuity Pathway Analysis (IPA) to quickly identify biological relationships, mechanisms, pathways, functions and diseases most relevant to experimental datasets. IPA is cited in tens of Read More...

09/15/2022 - Learn how to get started on using QIAGEN’s Ingenuity Pathway Analysis (IPA) to quickly identify biological relationships, mechanisms, pathways, functions and diseases most relevant to experimental datasets. IPA is cited in tens of Read More...

09/01/2022 - Learn how to get started on using QIAGEN’s Ingenuity Pathway Analysis (IPA) to quickly identify biological relationships, mechanisms, pathways, functions and diseases most relevant to experimental datasets. IPA is cited in tens of Read More...

06/15/2022 - Learn how to get started on using QIAGEN’s Ingenuity Pathway Analysis (IPA) to quickly identify biological relationships, mechanisms, pathways, functions and diseases most relevant to experimental datasets. IPA is cited in tens of Read More...

05/10/2022 - Overview Python is one of the preferred programming languages for scientists to solve a wide variety of biological problems. We find that many scientists who come to Software Carpentry workshops use Python and want Read More...

02/07/2022 - Learn how to get started on using QIAGEN’s Ingenuity Pathway Analysis (IPA) to quickly identify biological relationships, mechanisms, pathways, functions and diseases most relevant to experimental datasets. IPA is cited in tens of Read More...

Installing R, RStudio, and Rtools on Windows Goto https://www.r-project.org and click on "download R". Select a US mirror site to download from (for instance http://cran.wustl.edu/ ). Select " Read More...

10/14/2021 - Meeting Link Forward genetic screens using CRISPR (clustered regularly interspaced short palindromic repeats)–associated nucleases like Cas9 are a powerful tool to pinpoint genes involved in disease. Initial screens capitalized on genome-scale libraries to Read More...

09/01/2021 - Learn how to get started on using QIAGEN’s Ingenuity Pathway Analysis (IPA) to quickly identify biological relationships, mechanisms, pathways, functions and diseases most relevant to experimental datasets. IPA is cited in tens of Read More...

04/14/2021 - Register Session Description Learn how to get started on using QIAGEN’s Ingenuity Pathway Analysis (IPA) to quickly identify biological relationships, mechanisms, pathways, functions and diseases most relevant to experimental datasets. IPA is cited Read More...

11/05/2020 - Register Learn how to get started on using QIAGEN’s Ingenuity Pathway Analysis (IPA) to quickly identify biological relationships, mechanisms, pathways, functions and diseases most relevant to experimental datasets. IPA is cited in tens Read More...

06/16/2020 - The capability to unambiguously and comprehensively identify thousands of metabolites and other chemicals in clinical samples, including the microbiome, will revolutionize the search for environmental, dietary, and metabolic determinants of health and disease. By Read More...

05/28/2020 - Learn how to get started on using QIAGEN’s Ingenuity Pathway Analysis (IPA) to quickly identify biological relationships, mechanisms, pathways, functions and diseases most relevant to experimental datasets. IPA is cited in tens of Read More...

02/20/2020 - Learn how to easily analyze your gene expression data yourself - using Qlucore Omics Explorer NCI/CCR:To get access to Qlucore, put a request into NCI at Your Service under Get Help https:// Read More...

01/09/2020 - Description In this course, learn how to solve a real-world use case with machine learning and produce actionable results using Amazon SageMaker. This course teaches you how to use Amazon SageMaker to cover the Read More...

The Genomics Laboratory (formerly Laboratory of Molecular Technology) is an integrated, high-throughput molecular biology laboratory focusing on the development of genetics and genomics technologies, together with associated laboratory automation systems, data analysis, and information management Read More...

What is Visium FFPE v2 with CytAssist? Visium FFPE v2 is sequencing-based spatial profiling technology developed by 10x Genomics. This assay can take mouse or human tissue sections on normal glass slides as input and Read More...

The research conducted within the Synthetic Biologics Core (SBC) Facility has a dual role: Generate chemical biology tools and drug candidates for molecular targets identified by NCI research groups, Develop novel effective methods and tools Read More...

The NCI High-Throughput Imaging Facility (HiTIF) works in a collaborative fashion with NCI/NIH Investigators by providing them with the necessary expertise, instrumentation, and software to develop and execute advanced High-Throughput Imaging (HTI) assays. These Read More...

The Genomics and Pharmacology Facility is part of the NCI's Center for Cancer Research (CCR), within the Developmental Therapeutics Branch. Its mission is to manage and assess molecular interaction data obtained through multiple platforms, increase Read More...

Objectives Review the grammar of graphics template. Learn about the statistical transformations inherent to geoms. Learn more about fine tuning figures with labels, legends, scales, and themes. Learn how to save plots with ggsave() . Review Read More...

Data visualization with ggplot2 Objectives To learn how to create publishable figures using the ggplot2 package in R. By the end of this lesson, learners should be able to create simple, pretty, and effective figures. Read More...

Introduction to ggplot2 Objectives Learn the ggplot2 syntax. Build a ggplot2 general template. By the end of the course, students should be able to create simple, pretty, and effective figures. Data Visualization in the tidyverse Read More...

This page uses content directly from the Biostar Handbook by Istvan Albert. Obtain RNA-seq test data. The test data consists of two commercially available RNA samples: Universal Human Reference (UHR) and Human Brain Reference (HBR) . Read More...

Lesson 2: Getting Started with R on Biowulf Learning objectives Understand how R can be deployed on Biowulf Understand how to access and use R modules Learn to create a custom R library on Biowulf Deploying Read More...

Lesson 4: Submitting R Scripts via command line Learning Objectives Learn how to use R with less interaction Learn how to deploy sbatch R jobs, and learn about alternatives such as swarm . Learn about R job Read More...

Lesson 3: R Project Management and renv Learning objectives Discuss the importance of reproducibility Learn ways to make R analyses more reproducible Learn how to set up and organize an R project Learn how to use Read More...

Multi-figure panel Objectives Combine multiple plots into a single figure Learn how to use patchwork and cowplot The primary purpose of this lesson is to learn how to combine multiple figures into a single multi-panel Read More...

Objectives Combine multiple plots into a single figure Learn how to use aspects of cowplot and patchwork The primary purpose of this lesson is to learn how to combine multiple figures into a single multi-panel Read More...

Stat Transformations: Bar plots, box plots, and histograms Objectives Review the grammar of graphics template Learn about the statistical transformations inherent to geoms Review data types Create bar plots, box & whisker plots, and histograms. Read More...

/* Whole document: */ body{ font-family: Times; font-size: 16pt; } Stat Transformations: Bar plots, box plots, and histograms Objectives Review the grammar of graphics template Learn about the statistical transformations inherent to geoms Review data types Create bar Read More...

Introduction to ggplot2 Objectives Learn how to import spreadsheet data. Learn the ggplot2 syntax. Build a ggplot2 general template. By the end of the course, students should be able to create simple, pretty, and effective Read More...

Visualizing clusters with heatmaps Objectives Introduce the heatmap and dendrogram as tools for visualizing clusters in data. Learn to construct cluster heatmap using the package pheatmap . Learn how to save a non-ggplot2 plot. Introduce ggplotify Read More...

Scatter plots and plot customization Objectives Learn to customize your ggplot with labels, axes, text annotations, and themes. Learn how to make and modify scatter plots to make fairly different overall plot representations. Load a Read More...

Visualizing clusters with heatmaps Objectives Introduce the heatmap and dendrogram as tools for visualizing clusters in data. Learn to construct cluster heatmap using the package pheatmap . Learn how to save a non-ggplot2 plot. Introduce ggplotify Read More...

Visualizing clusters with heatmaps Objectives Introduce the heatmap and dendrogram as tools for visualizing clusters in data. Learn how to work with the package pheatmap . Learn how to save a non-ggplot2 plot. Introduce ggplotify to Read More...

Objectives Review the grammar of graphics template. Learn about the statistical transformations inherent to geoms. Learn more about fine tuning figures with labels, legends, scales, and themes. Learn how to save plots with ggsave() . Review Read More...

Data visualization with ggplot2 Objectives To learn how to create publishable figures using the ggplot2 package in R. By the end of this lesson, learners should be able to create simple, pretty, and effective figures. Read More...