Bethesda, MD
Core Facility
The Flow Cytometry Core (LGI) offers established technologies to support studies using flow cytometry and cell sorting. Established Technologies Applications that run on FACS Caliburs include: Immunophenotyping (up to 4-color), Intracellular markers, including cytokines and Read More...
Bethesda, MD
Collaborative
As a multi-user facility, the different instruments provide a wide range of imaging modes for EIB scientists, from standard immunohistochemistry, through brightfield and wide-field epifluorescence imaging, to highly complex live cell confocal microscopy and super-resolution Read More...
Frederick, MD
Core Facility
Clinical Support Laboratory – Flow Cytometry Section is a laboratory specializing in providing immunophenotyping support of NCI intramural clinical trials, though assessments may also be performed using cells from Non-human primates and other species. The CSL Read More...
Bethesda, MD
Core Facility
The LCBG Microscopy Core offers imaging technologies and training. The Core has established instrumentation for for 2D and 3D imaging of both fixed and living specimens.
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Back Services: Biophysics Facility offers ZetaView as an open-access instrument. First-time users must complete a short training session before using it for the first time. Training includes instrument calibration and size analysis of a standard Read More...
Bethesda, MD
Trans NIH Facility
The facilities at AIM are available for use by the entire NIH intramural research community. While we welcome users with any size imaging project, AIM specializes in large, yearlong (or longer), collaborative research efforts with Read More...
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Back Services: Biophysics Facility offers MDS as an open-access instrument. First-time users must complete a short training session before gaining access to the instrument reservation calendar. Training includes the KD determination of a standard molecular Read More...
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Bioinformatics
HBR_1 HBR_2 HBR_3 UHR_1 UHR_2 UHR_3 SULT4A1 375.0 343.6 339.4 3.5 6.9 2.6 MPPED1 157.8 158.4 162.6 0.7 3.0 2.6 PRAME 0.0 0.0 0.0 568.9 467.3 519.2 IGLC2 0.0 0.0 0.0 488.6 498.0 457.5 IGLC3 0.0 0.0 0.0 809.7 313.8 688.0
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Bioinformatics
HBR_1 HBR_2 HBR_3 UHR_1 UHR_2 UHR_3 SULT4A1 375.0 343.6 339.4 3.5 6.9 2.6 MPPED1 157.8 158.4 162.6 0.7 3.0 2.6 PRAME 0.0 0.0 0.0 568.9 467.3 519.2 IGLC2 0.0 0.0 0.0 488.6 498.0 457.5 IGLC3 0.0 0.0 0.0 809.7 313.8 688.0
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Bioinformatics
Explore this gene expression dataset a bit. How many samples (columns) and genes (row names) does this dataset have? {{Sdet}} Solution{{Esum}} This dataset contains 6 samples ( HBR_1.bam HBR_2.bam HBR_3.bam UHR_1.bam UHR_2. Read More...
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Confocal
ZEISS Elyra 7 with Lattice SIM² Type: Wide-field structured illumination microscope Capabilities: Super resolution 2D and 3D imaging of live or fixed cells Apotome SIM: 170 nm lateral, 450 nm axial Apotome SIM2: 140 nm lateral, 300 nm axial Lattice Read More...
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Bioinformatics
Here, R will be used to generate principal components plot for the HBR and UHR study. Principal components plots are a popular way to visualize how samples in RNA sequencing cluster based on gene expression. %% Read More...
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Confocal
Yokogawa CV8000 High-throughput spinning disk confocal microscope The Yokogawa CV8000 is HiTIF second high-throughput imaging system. The CV8000 feature set is similar to the CV7000.When compared to the latter, the CV8000 has a newer Read More...
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Confocal
Nikon SoRa Spinning Disk Capabilities: Inverted microscope Photo-metrics BSI sCMOS camera Yokogawa SoRa CSU-W1 spinning disk unit Super-resolution, confocal and wide-field imaging modes 4x, 10x, 20x and 60x objective lenses Mad City Labs 500 mm piezo Read More...
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Bioinformatics
The following questions will help you gain more confidence in exploring data through heatmap. We will work with a subset of the Human Brain Reference (HBR) and Universal Human Reference (UHR) RNA sequencing dataset and Read More...
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Confocal
Zeiss LSM980 with Airyscan 2 Confocal Microscope Inverted microscope Two PMTs and four GaAsP detectors for confocal imaging 4 color Airyscan 2 “super-resolution” detector Airyscan Multiplex modes for increased acquisition speed with high sensitivity 5x, 10x, 20x, 40x, 63 Read More...
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Confocal
2024 L. Balagopalan, T. Moreno, H. Qin, B. C. Angeles, T. Kondo, J. Yi, K. M. McIntire, N. Alvinez, S. Pallikkuth, M. E. Lee, H. Yamane, A. D. Tran, P. Youkharibache, R. E. Cachau, N. Taylor, Read More...
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Confocal
2024 Kim G, Chen Z, Li J, Luo J, Castro-Martinez F, Wisniewski J, Cui K, Wang Y, Sun J, Ren X, Crawford SE, Becerra SP, Zhu J, Liu T, Wang S, Zhao K, Wu Read More...
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Confocal
Zeiss LSM 880 NLO Laser Scanning Microscope Laser scanning microscope: 355nm UV laser 405nm, 594nm, 561nm and 633nm diode lasers Argon laser (458nm, 488nm and 514nm lines) Coherent Chameleon Vision II tunable (680nm – 1080nm) IR multiphoton Read More...
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Confocal
Confocal Microscope Image of Fixed Mouse Embryonic Fibroblast (MEF) Cells Actin proteins (stained red) and Tubulin proteins (stained green) are involved in a number of cellular process such as cell motility, cell division and maintenance Read More...
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Confocal
2024 Coutinho, L. L., Femino, E. L., Gonzalez, A. L., Moffat, R. L., Heinz, W. F., Cheng, R. Y. S., Lockett, S. J., Rangel, M. C., Ridnour, L. A. & Wink, D. A. NOS2 and Read More...
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Bioinformatics
Managing Bioinformatics Projects with Jupyter Lab Learning Objectives After this class, participants will have obtained the foundation needed to start using Jupyter Lab as an all-in-one place to maintain code, output, and other description of Read More...