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The Clinical Support Laboratory offers processing, tracking, and testing of a broad range of clinical samples. Support can begin at the early stages of clinical trial development to aid in developing a comprehensive strategy for Read More...

The Neurorehabilitation and Biomechanics Research Section (also referred to as the NAB LAB) is a multidisciplinary group of highly qualified scientists, clinical and technical staff, and trainees with diverse backgrounds including medical, physical therapy, neuroscience Read More...

CREx News & Updates March 2022 Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Site Spotlight NIA Nonhuman Primate Core The NIA Nonhuman Primate Core supports multi-disciplinary translational aging projects for Read More...

The Chemistry and Synthesis Center (CSC) of the National Heart, Lung, and Blood Institute (NHLBI) provides IRP scientists with targeted imaging probes and chemical tools that help accelerate cell-based assays, in vivo imaging studies, and Read More...

The Lasergene Molecular Biology package includes the following applications: SeqBuilder Pro, SeqMan Ultra, MegAlign Pro, GeneQuest, GenVision, SeqNinja, and DNASTAR Navigator. These applications provide tools to perform in silico gel electrophoresis and cloning, plasmid vector Read More...

02/05/2026 - Don’t miss this brand-new double-feature of two essential AI courses in one! We will start with AI Done Right: Ethics and Privacy to set the stage for understanding the AI landscape and the Read More...

08/20/2025 - Join this two-part AI course starting with AI Done Right: Ethics and Privacy to set the stage and ending with Prompt Like a Pro: Getting the Most from AI with Effective Prompt Engineering to Read More...

02/11/2022 - The seminar is open to the public and registration is required each month. Dr. Hunter Mosely plans to discuss the richness of open data resources in biology and how they can support machine learning Read More...

09/24/2025 - Don’t miss this brand-new double-feature of two essential AI courses in one! We will start with AI Done Right: Ethics and Privacy to set the stage for understanding the AI landscape Read More...

pwd You should see something like this. /home/username where username is your name. This is your home directory - where you start from when you open a terminal. This is an example of a & Read More...

06/22/2023 - The Bhattacharya Lab at the UCSF Parnassus Campus is focused on the functional role of monocyte-derived macrophages in the onset and persistence of fibrosis in the lung. We are addressing the following major questions, Read More...

09/30/2020 - Register Through large collaborative research projects, the field of neuroscience is gaining access to unprecedented amounts of information about the structure and function of the brain. These datasets are already having a profound impact Read More...

The cut command can be used to subset tabular data by column. To do this, specify the column number using -f option and the column separator using the -d option. For instance, to subset out Read More...

Caution "As with other keys, when you hit Enter or ↵ or on Macs, Return on your keyboard, your computer encodes this input as a character. Different operating systems use different character(s) to represent Read More...

Now that we have loaded the package, we can import our data. Generally, in R programming, functions that involve data import begin with "read / Read". Seurat includes a number of read functions for Read More...

We see that we are in our home directory. But where is that exactly? The file system on any computer is hierarchical, with the top level of the file system, or root directory, being / . See Read More...

A scripting language that can be used for manipulating data and generating reports. Awk is a utility that enables a programmer to write tiny but effective programs in the form of statements that define text Read More...

04/22/2021 - Presenter: Dr. Bernadette Redd is Lead Radiologist, Body MRI, Radiology and Imaging Sciences, at the NIH Clinical Center. Dr. Redd earned her Doctorate in Medicine from the Columbia University College of Physicians and Surgeons Read More...

08/21/2020 - Abstract: Machine learning (ML) has emerged as an essential tool for building models which can be used to predict clinical outcomes for age-related diseases. A significant challenge of ML is knowing which algorithms and Read More...

For tabular data in the form of csv files, which could contain multiple columns, the columns do not print to the terminal nicely aligned. The column command can fix this. The options and arguments in Read More...

To subset the counts for gene RABL2B in hbr_uhr_counts.csv, the awk command can be used. awk is one of the many Unix commands that are suitable for data processing. In the Read More...

For example, you may have noticed that our feature column from our example data is really two types of information combined (an Ensembl id and a gene abbreviation). If we want to separate this column Read More...

The final exercise in this lesson is to sort. To do this create text file called sorting_example.txt in the data directory. cd /data/username nano -L sorting_example.csv Then enter the following. Read More...

pwd pwd stands for "print working directory". When you run this, you should see something like this. /home/username where username is your name or student account. This is your home directory - Read More...

Let's use some functions. a. Use sum() to add the numbers from 1 to 10. {{Sdet}} Solution{{Esum}} sum ( 1 : 10 ) {{Edet}} b. Compute the base 10 logarithm of the elements in the following vector and save to an Read More...

Lesson 2 Exercise Questions: Base R syntax, objects, and data types Let's use some functions. a. Use sum() to add the numbers from 1 to 10. {{Sdet}} Solution{{Esum}} sum ( 1 : 10 ) {{Edet}} b. Compute the base 10 logarithm of Read More...

In tab delimited files, data columns are separated by tabs. To import tab-delimited files there are several options. There are base R functions such as read.delim() and read.table() as well as the readr Read More...

After the merged expression counts table has been created, we can proceed with differential expression analysis. Let's use DESeq2 again for this. But first, let's move counts.csv (the merged salmon expression table) Read More...

Retrieve R "helper" scripts developed for Biostars environment. curl -O http://data.biostarhandbook.com/rnaseq/code/deseq1.r curl -O http://data.biostarhandbook.com/rnaseq/code/deseq2.r curl -O http://data.biostarhandbook. Read More...

Retrieve R "helper" scripts developed for Biostars environment. curl -O http://data.biostarhandbook.com/rnaseq/code/deseq1.r curl -O http://data.biostarhandbook.com/rnaseq/code/deseq2.r curl -O http://data.biostarhandbook. Read More...

Lesson 2: Navigating file systems with Unix Quick review Unix is an operating system We use a unix shell (typically bash) to run many bioinformatics programs We need to learn unix to use non-GUI based tools Read More...

Let's use some functions. a. Use sum() to add the numbers from 1 to 10. {{Sdet}} Solution{{Esum}} sum ( 1 : 10 ) {{Edet}} b. Compute the base 10 logarithm of the elements in the following vector and save to an Read More...

Lesson 2 Exercise Questions: Base R syntax, objects, and data types Let's use some functions. a. Use sum() to add the numbers from 1 to 10. {{Sdet}} Solution{{Esum}} sum ( 1 : 10 ) {{Edet}} b. Compute the base 10 logarithm of Read More...

12/09/2022 - PhysioNet is a data sharing platform that began as an outreach component for an NIH research project in 1999. Rebuilt in 2019 following FAIR principles (Findable, Accessible, Interoperable, Reusable), the platform has grown rapidly. It now Read More...

Data analysis requires investigators to work with tabular data either in comma separated (csv) or tab separated format. Columns in a comma separated file are separated by commas. Those in tab separated files are separated Read More...

Go back to /data/user/hcc1395_b4b. cd /data/user/hcc1395_b4b Create a directory called hcc1395_featurecounts. mkdir hcc1395_featurecounts Go back into /data/user/hcc1395_b4b/hcc1395_hisat2 for this Read More...

This lesson provides an introduction to R in the context of single cell RNA-Seq analysis with Seurat. Learning Objectives Learn about options for analyzing your scRNA-Seq data. Learn about resources for learning R programming. Learn Read More...

Data import and reshape Objectives 1. Learn to import multiple data types 2. Data reshape with tidyr : pivot_longer() , pivot_wider() , separate() , and unite() Installing and loading packages So far we have only worked with objects that Read More...

Retrieve R "helper" scripts developed for Biostars environment. curl -O http://data.biostarhandbook.com/rnaseq/code/deseq1.r curl -O http://data.biostarhandbook.com/rnaseq/code/deseq2.r curl -O http://data.biostarhandbook. Read More...

Note that we now have differential expression by transcripts and our first column contains the transcript IDs. But what genes do these transcripts map to? We will need to do some data wrangling to find Read More...

Before we can align the HBR and UHR raw sequencing data to human chromosome 22 transcriptome, we need to create an index of this transcriptome (like we did with the genome). This will make the alignment Read More...

Lesson 7: Downloading the RNA-Seq Data and Dataset Overview Lesson Review pwd (print working directory) ls (list) touch (creates an empty file) nano (basic editor for creating small text files) using the rm command to remove Read More...

Lesson 16: RNA sequencing review and classification based analysis Before getting started, remember to be signed on to the DNAnexus GOLD environment. Review In the previous classes, we learned about the steps involved in RNA sequencing Read More...

This page contains content taken directly from the Biostar Handbook (Istvan Albert). Always remember to activate the class bioinformatics environment. conda activate bioinfo For this data analysis, we will be using: Two commercially available RNA Read More...

This page contains content taken directly from the Biostar Handbook (Istvan Albert). Always remember to activate the class bioinformatics environment. conda activate bioinfo For this data analysis, we will be using: Two commercially available RNA Read More...

This page uses content directly from the Biostar Handbook by Istvan Albert. Obtain RNA-seq test data. The test data consists of two commercially available RNA samples: Universal Human Reference (UHR) and Human Brain Reference (HBR) . Read More...

In tab delimited files, data columns are separated by tabs. To import tab-delimited files there are several options. There are base R functions such as read.delim() and read.table() as well as the readr Read More...

For tabular data in the form of csv files, which could contain multiple columns, the columns do not print to the terminal nicely aligned. The column command can fix this. The options and arguments in Read More...

Change into hcc1395_deg from hcc1395_b4b. cd hcc1395_deg List the contents. Use the -1 option to view directory contents 1 item per line. ls -1 The files generated from deg.R are: hcc1395_ Read More...