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Search Results for: solution scattering

Total Results Found: 127

Total Results Found: 127

Multi-Angle Light Scattering (MALS)

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Back Services: We offer a limited sample processing service using standard SEC-MALS and FFF protocols.  This service is intended for the occasional users of this system.  Researchers who expect to use this instrument Read More...

NHLBI Biophysics Core
Bethesda, MD

Core Facility

The Biophysics Core’s mission is to provide support in the study of macromolecular interactions, dynamics, and stability by offering consultations, training, professional collaborations, and instrument access. General Services Multi-technique molecular interaction studies, Kinetic and Read More...

Mass Photometer (MP) – Refeyn OneMP

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Back Services: Biophysics Facility offers MP as an open-access instrument.  First-time users must complete a short training session before gaining access to the instrument training calendar.  Training includes mass distribution analysis of a Read More...

NCI SAXS Facility
Frederick, MD

Collaborative

In order to meet increasing demands from both NIH intramural and extramural communities for access to a small angle X-ray scattering (SAXS) resource, the Center for Cancer Research (CCR) under the leadership of Drs. Jeffrey Read More...

Biophysics Core Technologies

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Home About the Biophysics Core Biophysics Core Services [tabby title="Instrumentation"] NHLBI Biophysics Core The Biophysics Core Facility: Overview Core Facilities provide scientific resources, cutting-edge technologies and novel approaches to support DIR scientists. Availability of Read More...

Center for Structural Biology: Biophysics Resource
Frederick, MD

Core Facility

The Biophysics Resource (BR) was established in January 2001. Our mission is to provide CCR investigators with access to both the latest instrumentation and expertise in characterizing the biophysical aspects of systems under structural investigation. The Read More...

Bio-Layer Interferometry (BLI) - Octet RED96

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Back Services: Biophysics Facility offers Octet as an open-access instrument.  First-time users must complete a short training session before gaining access to the instrument reservation calendar.  Training includes a full analysis of a Read More...

Microfluidic Diffusion Sizing (MDS) - Fluidity One-M

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Back Services: Biophysics Facility offers MDS as an open-access instrument. First-time users must complete a short training session before gaining access to the instrument reservation calendar. Training includes the KD determination of a standard molecular Read More...

NCI Center for Structural Biology: Crystallization Facility
Frederick, MD

Collaborative

The Crystallization Facility provides an automated environment for setting up crystallization experiments in a high-throughput format, storing the resulting plates under controlled conditions, and monitoring the status of prepared droplets remotely. The Facility is in Read More...

Isothermal Titration Calorimetry (ITC) – iTC200

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Back Services: Biophysics Facility offers ITC calorimeters as open-access instruments.  First-time users must complete a short training session before gaining access to the instrument reservation calendar.  Training includes performing a test experiment and Read More...

NIBIB Biomedical Engineering and Physical Science (BEPS)
Bethesda, MD

Trans NIH Facility

The Biomedical Engineering and Physical Science (BEPS) shared resource supports NIH’s intramural basic and clinical scientists on applications of engineering, physics, imaging, measurement, and analysis. BEPS is centrally located on the main NIH campus Read More...

May 2023 Newsletter

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CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More Technology Event Biophysical Methods for Protein Interactions Monday, May 15 – Friday, May 19, 2023 This workshop will review the strategies of Read More...

Fluorescence – PTI/Horiba QuantaMaster

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Back Services: Biophysics Facility offers fluorometers as open-access instruments.  First-time users must complete a short training session before gaining access to the instrument reservation calendar. Location: Building 50, room 3226 Description: Some substances reemit light after Read More...

Differential Scanning Calorimetry (DSC) – Microcal VP-DSC

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Back Services: Biophysics Facility offers DSC as an open-access instrument. First-time users must complete training before gaining access to the instrument reservation calendar. Location: Building 50, room 3123 Description: The differential scanning calorimeter measures the constant pressure Read More...

STRIDES Initiative
Bethesda, MD

Trans NIH Facility

The Science and Technology Research Infrastructure for Discovery, Experimentation, and Sustainability (STRIDES) Initiative The STRIDES Initiative aims to help NIH and its institutes, centers, and offices (ICOs) accelerate biomedical research by reducing barriers in utilizing Read More...

CCR Spatial Imaging Technology Resource (SpITR)
Bethesda, MD

Collaborative

The Spatial Imaging Technology Resource (formerly the Nanoscale Protein Analysis Section of the Collaborative Protein Technology Resource or CPTR) provides expertise and service in state-of-the-art protein analysis technologies to advance CCR research in basic discovery Read More...

Chemistry and Synthesis Center
Rockville, MD

Core Facility

The Chemistry and Synthesis Center (CSC) of the National Heart, Lung, and Blood Institute (NHLBI) provides IRP scientists with targeted imaging probes and chemical tools that help accelerate cell-based assays, in vivo imaging studies, and Read More...

R Introductory Series: Test your learning

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Bioinformatics

Which of the following functions is used to print your working directory in R? a. pwd b. Setwd() c. getwd() d. wkdir() {{Sdet}} Solution{{Esum}} C {{Edet}} Which of the following can be used to Read More...

R Introductory Series: Base R and data frames

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Bioinformatics

Lesson 3 Exercise Questions: BaseR dataframe manipulation and factors The filtlowabund_scaledcounts_airways.txt includes normalized and non-normalized transcript count data from an RNAseq experiment. You can read more about the experiment here . We are going Read More...

Research

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Confocal

Research Mission The goal of OMAC’s research is to understand molecular mechanisms driving carcinogenesis and the reversal of this process through treatment, by utilization and advancement of optical microscopy techniques. These techniques include Read More...

R Introductory Series: Test your learning

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Bioinformatics

Which of the following will NOT print the "Run" column from scaled_counts? a. scaled_counts$Run b. scaled_counts["Run"] c. scaled_counts[8,] d. scaled_counts[8] {{Sdet}} Solution{{Esum}} C {{ Read More...

R Introductory Series: Test your learning

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Bioinformatics

Given the following R code: fruit 678] c. Total_subjects(Total_subjects < 678) d. Total_subjects[Total_subjects < 678] {{Sdet}} Solution{{Esum}} D {{Edet}}

R Introductory Series: Test your learning

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Bioinformatics

From the interesting_trnsc data frame select the following columns and save to an object: sample, dex, transcript, counts_scaled. {{Sdet}} Possible Solution{{Esum}} interesting_trnsc_s

R Introductory Series: Practicing the Tidyverse (Part 1)

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Bioinformatics

Lesson 4 Exercise Questions: Tidyverse The filtlowabund_scaledcounts_airways.txt includes normalized and non-normalized transcript count data from an RNAseq experiment. You can read more about the experiment here . You can obtain the data outside of Read More...

R Introductory Series: Practicing the Tidyverse (Part 2)

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Bioinformatics

Lesson 5 Exercise Questions: Tidyverse The filtlowabund_scaledcounts_airways.txt includes normalized and non-normalized transcript count data from an RNAseq experiment. You can read more about the experiment here . You can obtain the data outside of Read More...

R Introductory Series: Practicing the Tidyverse

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Bioinformatics

Lesson 4 Exercise Questions: Tidyverse The filtlowabund_scaledcounts_airways.txt includes normalized and non-normalized transcript count data from an RNAseq experiment. You can read more about the experiment here . You can obtain the data outside of Read More...

R Introductory Series: Fix the axes so that the dimensions on the x-axis and the y-axis are equal. Both axes should start at 0. Label the axis breaks every 0.5 units on the y-axis and every 1.0 units on the x-axis.

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Bioinformatics

{{Sdet}} Solution{{Esum}} ggplot ( iris ) + geom_point ( aes ( Petal.Length , Petal.Width , color = Species )) + coord_fixed ( ratio = 1 , ylim = c ( 0 , 2.75 ), xlim = c ( 0 , 7 )) + scale_y_continuous ( breaks = c ( 0 , 0.5 , 1 , 1.5 , 2 , 2.5 )) + scale_x_continuous ( breaks = c ( 0 , 1 , 2 , 3 , 4 , 5 , 6 , 7 )) {{Edet}}

Microbiome Analysis with QIIME2: Practice Lesson 6

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Bioinformatics

This practice lesson is associated with Lesson 6 of the Microbiome Analysis with QIIME 2. In this practice lesson, we will view beta diversity results and determine whether our two conditions (old vs young) demonstrate significant differences Read More...

Microbiome Analysis with QIIME2: Practice Lesson 3

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Bioinformatics

This practice lesson is associated with Lesson 3 of the Microbiome Analysis with QIIME 2. In this practice lesson, we will work on generating a feature table and representative sequences. We will continue working with the data Read More...

Microbiome Analysis with QIIME2: Lesson 3: Denoising

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Bioinformatics

Practice Lesson 3 This practice lesson is associated with Lesson 3 of the Microbiome Analysis with QIIME 2. In this practice lesson, we will work on generating a feature table and representative sequences. We will continue working with Read More...

Microbiome Analysis with QIIME2: Lesson 5 Practice

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Bioinformatics

This practice lesson is associated with Lesson 5 of the Microbiome Analysis with QIIME 2. In this practice lesson, we will work on choosing a sampling depth to rarefy, running core-metrics, and comparing alpha diveristy between our Read More...

Data Wrangling with R: Practice problems

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Bioinformatics

Which of the following will throw an error and why? 4 _ chr :1:2: unexpected input ## 1: 4_ ## ^ . 4 chr :1:3: unexpected symbol ## 1: .4chr ## ^ {{Edet}} Create the following objects; give each object an appropriate name (your best guess at what name to Read More...

Microbiome Analysis with QIIME2: Practice Lesson 4

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Bioinformatics

This practice lesson is associated with Lesson 4 of the Microbiome Analysis with QIIME 2. In this practice lesson, we will work on filtering our feature table and representative sequences, classify our features, and generate a phylogenetic Read More...

Data Wrangling with R: Plotting with ggplot2

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Bioinformatics

For the following plots, let's use the diamonds data ( ?diamonds ). The diamonds dataset comes in ggplot2 and contains information about ~54,000 diamonds, including the price, carat, color, clarity, and cut of each diamond. --- R4 Read More...

Data Wrangling with R: Lesson 2: Help Session

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Bioinformatics

This is our first coding help session. We have designed some practice problems to get you acquainted with using R before beginning to wrangle in our next lesson. Practice problems Which of the following will Read More...

Microbiome Analysis with QIIME2: Summarize import

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Bioinformatics

{{Sdet}} Solution{{Esum}} qiime demux summarize \ --i-data 01_import/import.qza \ --o-visualization 01_import/import.qzv {{Edet}} Again, to view this file, you will need to move it to public . Note: It is easier to create the Read More...

Bioinformatics for Beginners 2022: Visualization

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Bioinformatics

Can we generate an expression heatmap? {{Sdet}} Solution{{Esum}} Rscript $CODE/create_heatmap.r {{Edet}} Next, let's generate the Principal Components Analysis plot. But first, we need to convert the counts.csv and design. Read More...

AI Club: Gene Set Analysis with Large Language Models

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Bioinformatics

04/14/2025 - Gene set analysis (GSA) is essential in genomic research, yet traditional methods often lack transparency and produce contextually irrelevant results, making interpretation challenging. While large language models (LLMs) offer a promising solution for result Read More...

BTEP course: Course Overview

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Bioinformatics

Partek Flow is a start-to-finish solution for analyzing high dimensional multi-omics sequencing data. It is a point-and-click software and is suitable for those who wish to avoid the steep learning curve associated with analyzing sequencing Read More...

R Introductory Series: Test your learning

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Bioinformatics

Create a data frame summarizing the mean counts_scaled by sample from the scaled_counts data frame. {{Sdet}} Possible Solution{{Esum}} scaled_counts |> group_by(sample) |> summarize(Mean_counts_scaled=mean(counts_scaled)) {{ Read More...

R Introductory Series: Test your learning

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Bioinformatics

Using mutate apply a base 10 logarithmic transformation to the counts_scaled column of sscaled . Save the resulting data frame to an object called log10counts. Hint: see the function log10() . {{Sdet}} Possible Solution{{Esum}} log10 Read More...

R Introductory Series: Test your learning

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Bioinformatics

Filter the interesting_trnsc data frame to only include the following genes: MCL1 and EXT1. {{Sdet}} Possible Solution{{Esum}} interesting_trnsc_f

R Introductory Series: Change to color of the points by species to be color blind friendly, and change the legend title to "Iris Species". Label the x and y axis to eliminate the variable names and add unit information.

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Bioinformatics

{{Sdet}} Solution{{Esum}} #multiple ways to find color blind friendly palettes. #using color brewer scales RColorBrewer :: display.brewer.all ( colorblindFriendly = TRUE ) ggplot ( iris ) + geom_point ( aes ( Petal.Length , Petal.Width , color = Species )) + coord_fixed ( ratio = 1 , Read More...

R Introductory Series: Test your learning

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Bioinformatics

Using what you have learned about select() and filter() , use the pipe ( |> ) to create a subset data frame from scaled_counts that only includes the columns 'sample', 'cell', 'dex', 'transcript', and 'counts_scaled' and Read More...

R Introductory Series: Play with the theme to make this a bit nicer. Change font style to "Times". Change all font sizes to 12 pt font. Bold the legend title and the axes titles. Increase the size of the points on the plot to 2. Bonus: fill the points with color and have a black outline around each point.

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Bioinformatics

{{Sdet}} Solution{{Esum}} ggplot ( iris ) + geom_point ( aes ( Petal.Length , Petal.Width , fill = Species ), size = 2 , shape = 21 ) + coord_fixed ( ratio = 1 , ylim = c ( 0 , 2.75 ), xlim = c ( 0 , 7 )) + scale_y_continuous ( breaks = c ( 0 , 0.5 , 1 , 1.5 , 2 , 2.5 )) + scale_x_continuous ( breaks = c ( 0 , 1 , 2 , 3 , 4 , 5 , 6 , 7 )) + scale_fill_ Read More...

Data Wrangling with R: Reshaping data

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Bioinformatics

Reshape iris_long to a wide format. Your new column names will contain names from Measurement.location . Your wide data should look as follows: ## # A tibble: 150 × 6 ## Iris.ID Species Sepal.Length Sepal.Width Petal.Length Read More...

Data Wrangling with R: Challenge Question

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Bioinformatics

Using the boxplot you created above, reorder the x-axis so that color is organized from worst (J) to best (D). There are multiple possible solutions. Hint: Check out functions in the forcats package (a tidyverse Read More...

Data Wrangling with R: Putting it all together

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Bioinformatics

Load in the comma separated file "./data/countB.csv" and save to an object named gcounts . {{Sdet}} Solution } gcounts `...1` colnames ( gcounts )[ 1 ] ## 1 Tspan6 703 567 867 71 970 242 ## 2 TNMD 490 482 18 342 935 469 ## 3 DPM1 921 797 622 661 8 500 ## 4 SCYL3 335 216 222 774 979 793 ## 5 FGR 574 574 515 584 941 344 ## 6 CFH 577 792 672 104 192 936 ## 7 FUCA2 798 766 995 27 756 546 ## 8 GCLC 822 874 923 705 667 522 ## 9 NFYA 622 793 918 868 334 64 {{Edet}} Plot the Read More...

Data Wrangling with R: Loading data

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Bioinformatics

Import data from the sheet "iris_data_long" from the excel workbook (file_path = "./data/iris_data.xlsx"). Make sure the column names are unique and do not contain spaces. Save Read More...

Data Wrangling with R: Help Session Lesson 5

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Bioinformatics

All solutions should use the pipe. Import the file "./data/filtlowabund_scaledcounts_airways.txt" and save to an object named sc . Create a subset data frame from sc that only includes the columns Read More...

Data Wrangling with R: Help Session Lesson 6

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Bioinformatics

Let's grab some data. library ( tidyverse ) acount_smeta % dplyr :: rename ( "Feature" = "...1" ) acount #differential expression results dexp % filter ( ! Feature %in% dexp $ feature ) ## # A tibble: 48,176 × 9 ## Feature SRR1039508 SRR1039509 SRR1039512 SRR1039513 SRR1039516 SRR1039517 ## ## 1 Read More...

Data Wrangling with R: Lesson 6: Continuing with Dplyr

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Bioinformatics

Help Session Lesson 6 Let's grab some data. library ( tidyverse ) acount_smeta % dplyr :: rename ( "Feature" = "...1" ) acount #differential expression results dexp % filter ( ! Feature %in% dexp $ feature ) ## # A tibble: 48,176 × 9 ## Feature SRR1039508 SRR1039509 SRR1039512 Read More...

Data Wrangling with R: Reshape challenge

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Bioinformatics

Use pivot_longer to reshape countB. Your reshaped data should look the same as the data below. {{Sdet}} Solution } library ( tidyverse ) countB % rownames_to_column ( "Feature" ) countB_l ## 1 Tspan6 1 703 71 ## 2 Tspan6 2 567 970 ## 3 Tspan6 3 867 242 ## 4 TNMD 1 490 342 ## 5 TNMD 2 482 935 ## 6 Read More...

Data Wrangling with R: Step 1: Load the data.

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Bioinformatics

Begin by loading the data and saving to an object named dmat . {{Sdet}} Solution } library(tidyverse) dmat ## 1 ENSG00000001630.… 6877. 6614 7058. 11305. ## 2 ENSG00000002016.… 283. 287. 287. 265. ## 3 ENSG00000002330.… 1946 1662 2121 608 ## 4 ENSG00000002834.… 17636 19333 18917 4583 ## 5 ENSG00000003056.… 3874 4107 4005 5741 ## 6 ENSG00000003393.… 2041 2150 2141 1687 ## 7 ENSG00000003989.… 279 345 305 18586 ## 8 ENSG00000004534.… 2695. 3031 2871 1948 ## 9 ENSG00000004838.… 42 52 39 61 ## 10 ENSG00000004848.… 1 0 0 18 ## # ℹ 9,990 more rows ## # ℹ 2 more variables: `5_Cell2_Rep2` , `6_Cell2_Rep3`

Microbiome Analysis with QIIME2: Phylogeny

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Bioinformatics

In addition to clasifying our organisms, we also want to reconstruct their phylogenetic relationships by generating a phylogenetic tree. We often assume that phylogenetic closeness can elucidate commonalities in phenotypic properties / functions, so it is Read More...

Past Seminars

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Confocal

2024 Date: Tuesday, October 15, 2024 Time and Location: 11 am EST, ZOOM (INVITATION BY LMIG LIST SERVER) Speaker: Dr. Diego Presman (U Buenos Aires) Title: “Insights on Glucocorticoid Receptor Activity Through Live Cell Imaging” Summary: Eucaryotic transcription factors ( Read More...

BTEP course: Course overview

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Bioinformatics

Course Overview Partek Flow is a start-to-finish solution for analyzing high dimensional multi-omics sequencing data. It is a point-and-click software and is suitable for those who wish to avoid the steep learning curve associated with Read More...

R Introductory Series: Basics of R Programming

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Bioinformatics

Objectives To understand some of the most basic features of the R language including: Creating R objects and understanding object types Using mathematical operations Using comparison operators Creating, subsetting, and modifying vectors By the end Read More...

R Introductory Series: Introduction to R and RStudio

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Bioinformatics

Learning Objectives To understand: 1. the difference between R and RStudioIDE. 2. how to work within the RStudio environment including: creating an Rproject and Rscript navigating between directories using functions obtaining help how R can enhance data Read More...

Data Wrangling with R: Challenge data load

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Bioinformatics

Load in a tab delimited file (file_path= "./data/WebexSession_report.txt") using read_delim() . You will need to troubleshoot the error message and modify the function arguments as needed. {{Sdet}} Solution } library ( Read More...

Microbiome Analysis with QIIME2: Beta Diversity

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Bioinformatics

Lesson 6 . Learning Objectives Introduce several beta diversity metrics Discover different ordination methods Learn about statistical methods that are applicable Beta diversity Beta diversity is between sample diversity. This is useful for answering the question, how Read More...