Flow Cytometry

The CCR Building 41 Flow Cytometry Core is a full-service facility within the Center for Cancer Research that supports over 150 users representing 26 laboratories. The Core Facility provides instrument and software training, technical expertise, assay development, and experimental design for all users. The Core also offers sample preparation protocols and reagent recommendations, including fluorochrome panel setups for each flow cytometer and reagent cross-reactivity among species. The Core Manager provides assistance with data analysis and figure preparation for flow cytometry data presentat… [learn more]

The Flow Cytometry Core (LGI) offers established technologies to support studies using flow cytometry and cell sorting. Established Technologies Applications that run on FACS Caliburs include: Immunophenotyping (up to 4-color), Intracellular markers, including cytokines and phosphoproteins, Cell cycle analysis using propidium iodide for mammalian cells and cytox green for yeast, Cell cycle-associated BRDU labeling of S-phase cells and phosphohistone H3 labeling of mitotic cells, Apoptosis by annexin V binding, TUNEL, caspase, or FLICA, Detection o… [learn more]

The Clinical Flow Cytometry Laboratory provides extensive support for NCI clinical protocols by providing diagnostic testing for leukemia and lymphoma in patients either on NCI clinical protocols or undergoing testing to determine eligibility for NCI protocols. The Unit provides clinical flow cytometric evaluation of blood, bone marrow, fine needle aspirates, tissue biopsies and body fluids for hematolymphoid neoplasia in NCI and NIH patients. The majority of flow cytometric testing performed by the Unit involves detection of minimal residual disease (patients are status post-therapy at outsid… [learn more]

The NCI Clinical Research Correlatives Core provides non-CLIA-certified spectral flow cytometric assays to support clinical trials conducted in the CCR. The core specializes in immunophenotyping and immune monitoring assays. Established Technologies Spectral flow cytometry (Cytek), Services offered include: Assistance with the experimental design, Assay development/panel optimization, Sample processing and preparation, Sample acquisition, Data analysis, Immunoprofiling assays offered include: “a la carte” CYTEK expandable antibody panel… [learn more]

The CCR-Frederick Flow Cytometry Core provides research support and cytometry instruments for the Frederick-CCR community, including analysis and sorting services, instrument maintenance, new user training, and technical consultation.  The lab is supported by the Cancer Innovation Lab of CCR. Typical Flow Cytometry Applications Immunophenotyping, multicolor identification of immune cell subsets, Apoptosis assays, including Annexin V, TUNEL, caspase activation, or mitochondrial membrane potential, Cell cycle (DNA content) analysis, Cell health and proliferation monitoring, … [learn more]

The NCI Research Flow Facility provides cell sorting and benchtop flow cytometry services to NCI investigators. Services are program-specific and are not available to all NCI or NIH investigators. Please inquire as to availability. Established Technologies Fluorescence-activated cell sorting (FACS), Benchtop polychromatic flow cytometry, Laser scanning cytometry, Full-spectrum cytometry, Developing Technologies Stem cell analysis, Apoptosis detection, Integration of new laser technology into existing instrumentation, including solid-state diode and diode-pumped (DPSS) l… [learn more]

The NIH Center for Human Immunology, Inflammation, and Autoimmunity (CHI) is a trans-NIH resource whose mission is to provide a collaborative hub of advanced translational immunology for NIH clinical and pre-clinical studies. This uniquely structured program provides centralized and efficient management of projects in full collaboration with NIH investigators to better understand human immune function and pathophysiology. Our team works closely to plan and optimize experimental workflows tailored to each investigator’s specific research goals; execute highly sophisticated technological proce… [learn more]