Flow Cytometry Core (LGI) offers established technologies to support studies using flow cytometry and cell sorting. Instrumentation 2 FACSCaliburs – 2 lasers (488, 635) LSRII – 5 lasers (355, 405, 488, 561, 635) LSR Fortessa – 4 lasers (355, 405, 488, 639) FACS Aria (standard) – 3 lasers (405, 488, 639) FACS Aria (SORP) – 3 lasers (355, 488, 639) MoFlo Astrios – 5 lasers (355, 405, 488, 561, 640) Established Technologies Applications that run on FACS Caliburs include: Immunophenotyping (up to 4-color) Intracellular … [learn more]

The Clinical Flow Cytometry Laboratory provides extensive support for NCI clinical protocols by providing diagnostic testing for leukemia and lymphoma in patients either on NCI clinical protocols or undergoing testing to determine eligibility for NCI protocols. The Unit provides clinical flow cytometric evaluation of blood, bone marrow, fine needle aspirates, tissue biopsies and body fluids for hematolymphoid neoplasia in NCI and NIH patients. The majority of flow cytometric testing performed by the Unit involves detection of minimal residual disease (patients are status post-therapy at ou… [learn more]

The NCI Vaccine Branch Flow Cytometry Core is a full-service facility within the Center for Cancer Research that supports over 150 users representing 26 laboratories. The Core Facility provides instrument and software training, technical expertise, assay development, and experimental design for all users. The Core also offers sample preparation protocols and reagent recommendations, including fluorochrome panel setups for each flow cytometer and reagent cross-reactivity among species. The Core Manager provides assistance with data analysis and figure preparation for flow cytometry data present… [learn more]

The CCR-Frederick Flow Cytometry Core Facility provides research support to the Frederick-CCR community, including cytometry analysis and sorting services, instrument maintenance, new user training, and technical consultation.Typical Assays Performed by Core InstrumentsImmunophenotyping of mouse and human cellsApoptosis assays, including Annexin V, TUNEL, caspase activation, or mitochondrial membrane potentialCell cycle (DNA content) analysisCell health and proliferation monitoringSterile Cell sorting (physical separation) for bulk collection or deposition in 96- or 384-well platesSorting … [learn more]

Clinical Support Laboratory – Flow Cytometry Section is a laboratory specializing in providing immunophenotyping support of NCI intramural clinical trials, though assessments may also be performed using cells from Non-human primates and other species. The CSL Flow cytometry core is operated by Leidos Biomedical Research Inc. on behalf of NCI as part of the Frederick National Laboratory. Contact Details Head: Jon Inglefield Phone: 301.846.6865 Email: jon.inglefield@nih.gov Address: Building 469, Room 107A, Frederick, MD 21702 Instrumentation BD FACSCanto II – 3 lasers (… [learn more]

The NCI Research Flow Facility provides cell sorting and benchtop flow cytometry services to NCI investigators. Services are program-specific and are not available to all NCI or NIH investigators. Please inquire as to availability. Established Technologies Fluorescence-activated cell sorting (FACS) Benchtop polychromatic flow cytometry Laser scanning cytometry Full-spectrum cytometry Developing Technologies Stem cell analysisApoptosis detectionIntegration of new laser technology into existing instrumentation, including solid-state diode and diode-pumped (DPSS) laser tec… [learn more]