Search Results for: LC-MS

03/07/2024 - This one-hour training will provide detailed information on how to create charts in MS Excel, including reviewing and selecting chart types, layouts, and styles. The training Read More...

03/06/2024 - Please join us on Wednesday, March 6 when Daniella Lowenberg from the University of California, California Digital Library will present “Defining the Need for Open Data Metrics.”   Widespread adoption Read More...

We will analyze these data by doing: 1. Alignment (hisat2) 2. Quantification (featureCounts) 3. Differential expression (DESeq) Use of a spike-in control Using a spike-in allows us to determine how well we can measure and reproduce data with Read More...

We will analyze these data by doing: 1. Alignment (hisat2) 2. Quantification (featureCounts) 3. Differential expression (DESeq) Use of a spike-in control Using a spike-in allows us to determine how well we can measure and reproduce data with Read More...

We will analyze these data by doing: 1. Alignment (hisat2) 2. Quantification (featureCounts) 3. Differential expression (DESeq) Use of a spike-in control Using a spike-in allows us to determine how well we can measure and reproduce data with Read More...

“Gene set enrichment analysis” refers to the process of discovering the common characteristics potentially present in a list of genes. When these characteristics are GO terms, the process is called “functional enrichment.” Warning Overall GO Read More...

“Gene set enrichment analysis” refers to the process of discovering the common characteristics potentially present in a list of genes. When these characteristics are GO terms, the process is called “functional enrichment.” Warning Overall GO Read More...

There should not be spaces in Unix file names or directories. Here is a good method to use: Use the underscore (_) where a space would go, like this, to name a directory containing RNA-Seq data. Read More...

We will use the R helper scripts that we used before. cat 22simple_counts.txt | Rscript deseq2.r 3x3 > 22results_deseq2.txt The file 22results_deseq2.txt contains the genes sorted by their adjusted Read More...

We will use the R helper scripts that we used before. cat 22simple_counts.txt | Rscript deseq2.r 3x3 > 22results_deseq2.txt The file 22results_deseq2.txt contains the genes sorted by their adjusted Read More...

We will use the R helper scripts that we used before. cat 22simple_counts.txt | Rscript deseq2.r 3x3 > 22results_deseq2.txt The file 22results_deseq2.txt contains the genes sorted by their adjusted Read More...

07/28/2021 - Register/Join During this month's NCI Cancer Research Data Commons (CRDC) Cancer Genomics Cloud (CGC)  webinar series, two Georgetown University staff members about leveraging the CGC in their  data science  courses and curriculum. Read More...

05/25/2021 - Registration is required. Register at this link . Python is a good programming language for scientists to solve a wide variety of biological problems. We find that many scientists who come to Software Carpentry workshops Read More...

05/18/2021 - Registration is required. Register at this link . Python is a good programming language for scientists to solve a wide variety of biological problems. We find that many scientists who come to Software Carpentry workshops Read More...

05/11/2021 - Registration is required. Register at this link . Python is a good programming language for scientists to solve a wide variety of biological problems. We find that many scientists who come to Software Carpentry workshops Read More...

05/04/2021 - Registration is required. Register at this link . Python is a good programming language for scientists to solve a wide variety of biological problems. We find that many scientists who come to Software Carpentry workshops Read More...

04/28/2021 - Register/Join This month’s Cancer Genomics Cloud (CGC) webinar welcomes two bioinformaticians, Dr. Vesna Pajic and Nevena Vukojicic, to show attendees how CGC features can be used to perform multi-omics analysis. Dr. Pajic Read More...

04/27/2021 - Registration is required. Register at this link . Python is a good programming language for scientists to solve a wide variety of biological problems. We find that many scientists who come to Software Carpentry workshops Read More...

10/19/2020 - Register This webinar is intended for cancer researchers who are interested in learning more about the data that is available in the NCI Genomic Data Commons (GDC).The GDC is a unified repository that Read More...

We are going to place the FASTQC html reports for SRR1553606 in our Windows Downloads folder. The next step is to change into the Windows Downloads folder. Upon opening the command prompt, you should be Read More...

There should not be spaces in Unix file names or directories. There are many strategies that can be used to avoid spaces in file names. Though, consistency is key. One good method is using snake_ Read More...

Mass Spectrometry Section of the Collaborative Protein Technology Resource (Bldg. 37) Core Capabilities: Identification of proteins in complexes, organelles, subcellular fractions, or fluids. Global relative protein quantitation. Quantitation by isotopic labeling of cells in culture (SILAC) Read More...

The FNLCR Molecular Histopathology Laboratory (MHL) provides comprehensive veterinary pathology support for animal health monitoring, biomarker discovery and validation, drug development, genomics, and proteomics on a cost recovered basis. The MHL is organized into multiple Read More...

The Mass Spectrometry Unit of the Collaborative Protein Technology Resource uses mass spectrometry for the study of proteins. We perform collaborative experiments with CCR researchers in areas such as: interactomes, quantitative global proteome analyses, and Read More...

09/30/2024 - In this webinar, you'll gain insights into the Electronic Medical Record Search Engine (EMERSE). EMERSE is a simple, powerful tool to help researchers like you identify key data within free text clinical notes Read More...

This page contains content taken directly from the Biostar Handbook by Istvan Albert. Always remember to start the bioinformatics environment. conda activate bioinfo We will be analyzing differential expression of genes on Chr22 from the Read More...

This page contains content taken directly from the Biostar Handbook by Istvan Albert. Always remember to start the bioinformatics environment. conda activate bioinfo We will be analyzing differential expression of genes on Chr22 from the Read More...

You will need: active, unlocked Biowulf account (hpc.nih.gov) active Globus account for transferring files OR set up a file transfer program ( Filezilla ) for Mac or WinSCP for Windows PC. program to establish a Read More...

Retrieve R "helper" scripts developed for Biostars environment. curl -O http://data.biostarhandbook.com/rnaseq/code/deseq1.r curl -O http://data.biostarhandbook.com/rnaseq/code/deseq2.r curl -O http://data.biostarhandbook. Read More...

Retrieve R "helper" scripts developed for Biostars environment. curl -O http://data.biostarhandbook.com/rnaseq/code/deseq1.r curl -O http://data.biostarhandbook.com/rnaseq/code/deseq2.r curl -O http://data.biostarhandbook. Read More...

From the publication REDO: Genomic surveillance elucidates Ebola virus origin and transmission during the 2014 outbreak First we get the project (PRJN) number from the publication: PRJNA257197 Next we're going to query the "sra& Read More...

Retrieve R "helper" scripts developed for Biostars environment. curl -O http://data.biostarhandbook.com/rnaseq/code/deseq1.r curl -O http://data.biostarhandbook.com/rnaseq/code/deseq2.r curl -O http://data.biostarhandbook. Read More...

This page uses material directly from the Biostar Handbook by Istvan Albert. Always remember to activate the bioinformatics environment. conda activate bioinfo To align sequences to a genome, we need (1) genome sequence and the (2) sequence Read More...

Unix, what is it, and why should biologists take the time to learn it? The Unix operating system forms the basis of many bioinformatics analyses resources, such as the NIH High Performance Cluster (HPC) Biowulf/ Read More...

11/16/2022 - Attend this webinar to hear a moderated, five-person panel expand on the presentation, “Perspectives on CMS Linkage for Cancer Research in Cohort Studies.” This presentation is from the recent 2022 Annual Meeting of the NCI Read More...

09/27/2022 - Speaker: Neil L. Kelleher, Ph.D. Walter and Mary E. Glass Professor of Molecular Biosciences Professor of Chemistry in the Weinberg College of Arts and Sciences Professor of Medicine (Hematology & Oncology) in Read More...

04/06/2022 - Life Rafts in a Sea of Data: The Role of Librarians in Supporting Data Sharing Abstract:  The NIH has updated its policies on managing and sharing research data and will require a Data Management Read More...

02/11/2022 - The seminar is open to the public and registration is required each month. Dr. Hunter Mosely plans to discuss the richness of open data resources in biology and how they can support machine learning Read More...

04/20/2021 - Registration is required. Register at this link . Before the workshop: We will use Google Colab for this workshop. Colab allows us to write and execute Python code through the browser. A Google Account is Read More...

03/05/2021 - Overview: The Human Cell Atlas (HCA) is an ambitious global initiative that aims to create a comprehensive reference map of all human cells—the fundamental units of life—as a basis for both understanding Read More...

Protein Characterization Laboratory (PCL) offers various technologies to CCR investigators to characterize proteins and metabolites. The laboratory develops and applies state-of-the-art analytical technologies, primarily mass spectrometry, liquid chromatography, and Surface Plasmon Resonance (SPR), to advance Read More...

Protein Characterization Laboratory (PCL) offers various technologies to CCR investigators to characterize proteins and metabolites. The laboratory develops and applies state-of-the-art analytical technologies, primarily mass spectrometry, liquid chromatography, and Surface Plasmon Resonance (SPR), to advance Read More...

The Pathology Service, Division of Veterinary Resources (DVR), Office of Research Services (ORS), provides diagnostic gross and histopathologic evaluation of any species of laboratory animals used in the NIH Intramural Research Program due to morbidity, Read More...

Proteomic Data Common (PDC) represents the NCI’s largest public repository of proteogenomic comprehensive tumor datasets, essentially a Proteogenomic Cancer Atlas. It was developed to advance our understanding of how proteins help shape the risk, Read More...

The CCR Collaborative Bioinformatics Resource (CCBR) is a centrally funded resource group which provides a mechanism for CCR researchers to obtain many different types of bioinformatics assistance to further their research goals. The group has Read More...

[tabby title="Home"] About NICE-NIH Intramural CryoEM Consortium  NIH Intramural CryoEM Consortium (NICE) serves intramural investigators in all NIH IC’s. NICE provides access to state-of-the-art Titan Krios cryo-electron microscopes for atomic-resolution structure determination of Read More...

The Chemistry and Synthesis Center (CSC) of the National Heart, Lung, and Blood Institute (NHLBI) provides IRP scientists with targeted imaging probes and chemical tools that help accelerate cell-based assays, in vivo imaging studies, and Read More...

The OSTR offers cutting-edge technology platforms to the CCR scientific community through centralized facilities. The videos accessed through this page are designed to introduce the various scientific methodologies OSTR makes available through the cores on Read More...

Training Contact the LCBG Microscopy Core to discuss your experiment: Meet with Ross Lake, Core Head, to discuss your specific project. If the project is feasible, schedule an appointment to receive training and use the Read More...

2024 Senatorov IS, Bowman J, Jansson KJ, Alilin AN, Capaldo BJ, Lake R, Riba M, Abbey YC, Mcknight C, Zhang X, Raj S, Beshiri ML, Shinn P, Ngyuyen H, Thomas CJ, Corey E, Kelly K. Castrate Read More...

Techniques The Laboratory of Cancer Biology and Genetics Microscopy Core houses multiple systems that can be used to analyze cell structure, protein expression, and cell dynamics using immunofluorescence. These include inverted epifluorescence microscopes, a confocal Read More...

Our Team Tatiana S. Karpova Ph.D.Core Headkarpovat@nih.govBuilding 41, Room C615240-760-6637 David A. Ball Ph.D.Core Biologistballa@nih.govBuilding 41, Room B114D240-760-6577 Mohamadreza Fazel, Ph.D.Core Biologistmohamadreza. Read More...

2016-2024 Publications using images obtained in OMC

Software Image Acquisition Commercial imaging systems of LRBGE Optical Microscopy Core are controlled by acquisition software specifically designed for the appropriate microscope, such as ZEN (Zeiss confocal microscopes), Nikon Elements (Nikon), Imspector (Abberior). Custom-built HILO Read More...

Software LAS Leica Application Suite X (LAS X) is the one software platform for all Leica microscopes: It integrates confocal, wide field, stereo, super-resolution, and light-sheet instruments from Leica Microsystems. MetaMorph The MetaMorph® Microscopy Automation Read More...

Leica SP8 LSCM with white light laser The SP8 LIGHTNING confocal microscope allows you to make proper and detailed observations of fast biological processes. Your experimental work will have the benefit of super-resolution, high-speed imaging, Read More...

2024 Kim G, Chen Z, Li J, Luo J, Castro-Martinez F, Wisniewski J, Cui K, Wang Y, Sun J, Ren X, Crawford SE, Becerra SP, Zhu J, Liu T, Wang S, Zhao K, Wu Read More...

2025 Sebastian R, Sun EG, Fedkenheuer M, Fu H, Jung S, Thakur BL, Redon CE, Pegoraro G, Tran AD, Gross JM, Mosavarpour S, Kusi NA, Ray A, Dhall A, Pongor LS, Casellas R, Aladjem MI. Mechanism Read More...

Confocal Microscope Image of Fixed Mouse Embryonic Fibroblast (MEF) Cells Actin proteins (stained red) and Tubulin proteins (stained green) are involved in a number of cellular process such as cell motility, cell division and maintenance Read More...

2024 Coutinho, L. L., Femino, E. L., Gonzalez, A. L., Moffat, R. L., Heinz, W. F., Cheng, R. Y. S., Lockett, S. J., Rangel, M. C., Ridnour, L. A. & Wink, D. A. NOS2 and Read More...

Image Analysis Capabilities: Custom Development Software Supported Software Platforms Arivis Vision 4D Arivis is software for image processing, analysis, and display for working with multi-channel 2D, 3D and 4D images of almost unlimited size independent Read More...

Research Mission The goal of OMAL’s research is to understand molecular mechanisms driving carcinogenesis and the reversal of this process through treatment, by utilization and advancement of optical microscopy techniques. These techniques include multiplex Read More...

Nikon SoRa Spinning Disk Capabilities: Inverted microscope Photo-metrics BSI sCMOS camera Yokogawa SoRa CSU-W1 spinning disk unit Super-resolution, confocal and wide-field imaging modes 4x, 10x, 20x and 60x objective lenses Mad City Labs 500 mm piezo Read More...

General Microscopy Resources Confocal Listserv Email discussion list focused on confocal microscopy, but also including topics on fluorescence microscopy and digital imaging. MicroscopyU Online learning platform by Nikon.  An online source for Microscopy education. Leica Read More...

2024 Date: Tuesday, October 15, 2024 Time and Location: 11 am EST, ZOOM (INVITATION BY LMIG LIST SERVER) Speaker: Dr. Diego Presman (U Buenos Aires) Title: “Insights on Glucocorticoid Receptor Activity Through Live Cell Imaging” Summary: Eucaryotic transcription factors ( Read More...

Bolyen E, Rideout JR, Dillon MR, Bokulich NA, Abnet CC, Al-Ghalith GA, Alexander H, Alm EJ, Arumugam M, Asnicar F, Bai Y, Bisanz JE, Bittinger K, Brejnrod A, Brislawn CJ, Brown CT, Callahan BJ, Caraballo-Rodrí Read More...

References This course series primarily used information from QIIME2.org and the QIIME2 forum . Specifically, this course series focused on data and code from the QIIME2 Cancer Microbiome Intervention Tutorial . Special thanks goes to the Read More...

This page contains content taken directly from the Biostar Handbook (Istvan Albert). Always remember to activate the class bioinformatics environment. conda activate bioinfo For this data analysis, we will be using: Two commercially available RNA Read More...

This page contains content taken directly from the Biostar Handbook (Istvan Albert). Always remember to activate the class bioinformatics environment. conda activate bioinfo For this data analysis, we will be using: Two commercially available RNA Read More...

First Unix command (ls) ls You may see something like this: public reads.tar sample.fasta sample.fastq The "ls" command "lists" the contents of the directory you are in. You Read More...

First Unix command (ls) ls You may see something like this: public reads.tar sample.fasta sample.fastq The "ls" command "lists" the contents of the directory you are in. You Read More...

First Unix command (ls) ls You may see something like this: public reads.tar sample.fasta sample.fastq The "ls" command "lists" the contents of the directory you are in. You Read More...

First Unix command (ls) ls You may see something like this: public reads.tar sample.fasta sample.fastq The "ls" command "lists" the contents of the directory you are in. You Read More...

Why Learn Bioinformatics? Analyze your own data Expand scientific training and skills Provide a path to a new career Have a better understanding of how other people analyze data What is Unix? an operating system, Read More...

Why Learn Bioinformatics? Analyze your own data Expand scientific training and skills Provide a path to a new career Have a better understanding of how other people analyze data What is Unix? an operating system, Read More...

This page uses content directly from the Biostar Handbook by Istvan Albert. Review: * downloading data from SRA * decompressing tar files * e-utilities * fastq-dump Learn: * sra-stat * XML format * automating SRA downloads * working with comma-separated values (csv) format * Read More...

This page uses content directly from the Biostar Handbook by Istvan Albert. Obtain RNA-seq test data. The test data consists of two commercially available RNA samples: Universal Human Reference (UHR) and Human Brain Reference (HBR) . Read More...

Why Learn Bioinformatics? Analyze your own data Expand scientific training and skills Provide a path to a new career Have a better understanding of how other people analyze data What is Unix? an operating system, Read More...

Why Learn Bioinformatics? Analyze your own data Expand scientific training and skills Provide a path to a new career Have a better understanding of how other people analyze data What is Unix? an operating system, Read More...

“Gene set enrichment analysis” refers to the process of discovering the common characteristics potentially present in a list of genes. When these characteristics are GO terms, the process is called “functional enrichment.” Warning Overall GO Read More...

How to download data from the Sequence Read Archive (NCBI/SRA) to your account on NIH HPC Biowulf You will need: active, unlocked Biowulf account (hpc.nih.gov) active Globus account for transferring files OR Read More...

Lesson 2: Navigating file systems with Unix Quick review Unix is an operating system We use a unix shell (typically bash) to run many bioinformatics programs We need to learn unix to use non-GUI based tools Read More...

01/07/2016 - REGISTRATION IS FULL FOR THIS WORKSHOP (25 ATTENDEES; 6 WAITLIST) The Cancer Genome Atlas (TCGA) is a large-scale study that has cataloged genomic data accumulated for many different types of cancers, and includes mutations, copy number Read More...

10/22/2015 - /* element spacing */ p, pre { margin: 0em 0em 1em; } /* center images and tables */ img, table { margin: 0em auto 1em; } p { text-align: justify; } tt, code, pre { font-family: 'DejaVu Sans Mono', 'Droid Sans Mono', 'Lucida Console', Consolas, Read More...

01/29/2015 - /* element spacing */ p, pre { margin: 0em 0em 1em; } /* center images and tables */ img, table { margin: 0em auto 1em; } p { text-align: justify; } tt, code, pre { font-family: 'DejaVu Sans Mono', 'Droid Sans Mono', 'Lucida Console', Consolas, Read More...

First Unix command (ls) ls You may see something like this: public reads.tar sample.fasta sample.fastq The "ls" command "lists" the contents of the directory you are in. You Read More...

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How do I determine which EM technique should be used for my research? Schedule a free consultation with the Head of EML, Dr. Ru-ching Hsia, to discuss the aim of your research project. Dr.& Read More...

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