Search Results for: cytokine release assay

https://bioconductor.org/packages/release/bioc/html/IsoformSwitchAnalyzeR.html

The Clinical Support Laboratory offers processing, tracking, and testing of a broad range of clinical samples. Support can begin at the early stages of clinical trial development to aid in developing a comprehensive strategy for Read More...

As you can see from the image, there are several accessor functions to access the data from the object: assays() - access matrix-like experimental data (e.g., count data). Rows are genomic features (e.g., Read More...

Seurat v5 introduced the following new features: Integrative multi-modal analysis with bridge integration ‘Sketch’-based analysis of large data sets methods for spatial transcriptomics assay layers You can read about major changes between Seurat v5 Read More...

The Seurat Object is a data container for single cell RNA-Seq and related data. It is an S4 object, which is a type of data structure that stores complex information (e.g., scRNA-Seq count matrix, Read More...

Now, let's take a look at the number of detected features. VlnPlot(adp, features = "nFeature_RNA", group.by="orig.ident") + scale_fill_manual(values=cnames) Warning: Default search for " Read More...

Bioconductor is both an open source project and repository for R packages related to the analysis of biological data, primarily bioinformatics and computational biology, and as such it is a great place to search for Read More...

R is both a computational language and environment for statitical computing and graphics. It is open-source and widely used, not just by bioinformaticians. R is a particularly great resource for statistical analysis, plotting, and report Read More...

One way of producing a heatmap is to use geom_tile in ggplot2. However, the process becomes cumbersome if we want to include a dendrogram to show clustering. To construct a heatmap and dendrogram using Read More...

07/09/2020 - The recent breakthroughs in high-throughput technologies have resulted in a vast amount of big-data resources. However, it remains a significant challenge to transfer the knowledge from the public data to a new research project Read More...

Scatterplots are useful for visualizing treatment–response comparisons, associations between variables, or paired data (e.g., a disease biomarker in several patients before and after treatment). - Holmes and Huber, 2021 Because scatter plots involve mapping Read More...

What function would we use to transform table A to table B? Table A: # A tibble: 19 × 12 fish Release I80_1 Lisbon Rstr Base_TD BCE BCW BCE2 BCW2 MAE MAW <fct> & Read More...

Be sure to stay in your data directory for this exercise. In your data directory, create a folder called srr1553423_fastqc. mkdir srr1553423_fastqc We are going to download sequencing data for NCBI SRA study Read More...

After this lesson, we will Be able to develop a short shell script that will download sequencing data from NCBI SRA assay the quality of the sequencing data Know how to submit shell scripts as Read More...

https://bioconductor.org/packages/release/bioc/html/DEXSeq.html These tools serve different purposes within the RNA-Seq analysis workflow. rMATS (that would be discussed today) is tailored for identifying differential alternative splicing events, IsoformSwitchAnalyzeR focuses Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More New CREx User Survey The CREx Team is carrying out a CREx User Survey. We would greatly appreciate Read More...

Biomarker Discovery with Morphological Context:  Changing how tissue specimens are analyzed < https://youtu.be/mVhfZq8ppbc What is Digital Spatial Profiling? GeoMx Digital Spatial Profiler is a novel platform developed by NanoString. Digital Spatial Read More...

The Spatial Imaging Technology Resource (formerly the Nanoscale Protein Analysis Section of the Collaborative Protein Technology Resource or CPTR) provides expertise and service in state-of-the-art protein analysis technologies to advance CCR research in basic discovery Read More...

CREx Monthly Newsletter Learn about the NIH Collaborative Research Exchange (CREx), Core Facilities, Webinars, & More New CREx User Survey The CREx Team is carrying out a CREx User Survey. If you haven’t Read More...

The CCR Building 41 Flow Cytometry Core is a full-service facility within the Center for Cancer Research that supports over 150 users representing 26 laboratories. The Core Facility provides instrument and software training, technical expertise, assay development, and Read More...

03/20/2025 - Please note: Registration is required to get the Meeting Link for this event. Please pre-register. BTEP and the Single Cell and Spatial Transcriptomics Interest Group jointly present: Quantifying spatiotemporal dynamics during embryogenesis is crucial Read More...

10/08/2024 - We will cover recently developed statistical approaches to the classification of BRCA2 variants using the functional-assay data generated by our NCI collaborators (Dr. Sharan’s lab). Our main objective is to illustrate how statistical Read More...

VlnPlot(adp, features = "percent.mt", group.by="orig.ident") + scale_fill_manual(values=cnames) + geom_hline(yintercept=10,color="red") Warning: Default search for "data" layer in " Read More...

The metadata in the Seurat object is located in adp@metadata and contains the information associated with each cell. We can access the metadata using: head(adp@meta.data) #using head to return only the Read More...

Some tools have been described in the previous session (see here ). Today, we will be focusing on the SingleR tool, which also requires the celldex package . In short, SingleR operates by comparing your current dataset Read More...

The total number of detected transcripts expressed in a cell is dependent on the amount of mRNA in a cell. Cells naturally vary in the total amount of mRNA expressed. However, the chemistry of the Read More...

S4 is a rigorous system that forces you to think carefully about program design. It’s particularly well-suited for building large systems that evolve over time and will receive contributions from many programmers. --- Advanced Read More...

06/15/2023 - Since its release in September 2005, the Gene Set Enrichment Analysis (GSEA) software and its companion gene set collections, the Molecular Signatures Database (MSigDB), have been invaluable resources for interpreting the biological significance of expression Read More...

11/17/2022 - Join Icahn School of Medicine at Mount Sinai’s Dr. Zeynep Gümüş to learn about a user-friendly tool she and her team are developing to enable researchers of all computational skill levels to Read More...

11/09/2022 - Since its release in September 2005, the Gene Set Enrichment Analysis (GSEA) software and its companion gene set collections, the Molecular Signatures Database (MSigDB) have served as key resources for interpreting the biological significance of Read More...

09/16/2021 - In 2001, Celera Genomics and the International Human Genome Sequencing Consortium published their initial drafts of the human genome, which revolutionized the field of genomics. While these drafts and the updates that followed effectively covered Read More...

09/14/2021 - The workshop focuses on using the Atom Modeling PipeLine (AMPL), an open-source conda-based software that automates key drug discovery steps. AMPL is designed to take molecular binding data (IC50, ki) and carry out the Read More...

10/28/2020 - Register The NCI’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) is a national effort to accelerate the understanding of the molecular basis of cancer through the application of large-scale proteogenomics. The next TEAG forum Read More...

10/08/2020 - Register MacVector is a powerful but easy to use Macintosh application that lets you document, analyze and manipulate DNA and Protein sequences. A simple graphical interface lets you generate beautiful plasmid maps, design primers, Read More...

02/27/2020 - In this symposium, distinguished scientists will share recent successes in their research to visualize RNA targets at the single molecule level with single cell resolution and morphological context. Here are some of the exciting Read More...

rMATS is a computational tool for detection and quantification of differential alternative splicing events. rMATS stands for "replicate Multivariate Analysis of Transcript Splicing". After its initial release in 2011, it saw continuous development and Read More...

rMATS is a computational tool for detection and quantification of differential alternative splicing events. rMATS stands for "replicate Multivariate Analysis of Transcript Splicing". After its initial release in 2011, it saw continuous development and Read More...

The Molecular Targets Program (MTP) is an organizational entity within the Center for Cancer Research (CCR) at NCI. The MTP provides the focus and infrastructure to enable CCR tenured and tenure-track Principal Investigators to initiate Read More...

The NCI Clinical Research Correlatives Core provides non-CLIA-certified spectral flow cytometric assays to support clinical trials conducted in the CCR. The core specializes in immunophenotyping and immune monitoring assays. Established Technologies Spectral flow cytometry (Cytek), Read More...

Back Services: Biophysics Facility offers Tycho as an open-access instrument.  This instrument is very easy to use, and no formal training is required.  Core staff will help with their first experiment of new Read More...

NCI established the Nanotechnology Characterization Laboratory (NCL) to support the extramural research community to accelerate the progress of nanomedicine by providing preclinical characterization and safety testing of nanoparticles. It is a collaborative effort between NCI, Read More...

The Functional Genomics Laboratory (formerly, the RNAi Screening Facility) of the National Center for Advancing Translational Sciences (NCATS) assist investigators with all stages of project planning and execution, beginning with assay development through genome-wide siRNA Read More...

The Trans-NIH Metabolomics Core (TNMC) is a shared research resource that performs metabolomics analysis (and related small molecule research) for investigators at all Institutes and Centers across the NIH Intramural Research Program. The TNMC is Read More...

NCI LASP Animal Research Technology Support (ARTS) provides customized technical support for basic and translational animal-based research to the scientific community. We offer a wide array of services ranging from expert colony management to the Read More...

What is Xenium? Xenium is a high-resolution, imaging-based in situ spatial profiling technology from 10x Genomics that allows for simultaneous expression analysis of RNA targets (currently in range of 100’s) within the same tissue section. Read More...

The NIH Center for Human Immunology, Inflammation, and Autoimmunity (CHI) is a trans-NIH resource whose mission is to provide a collaborative hub of advanced translational immunology for NIH clinical and pre-clinical studies. This uniquely structured Read More...

Objectives To explore Bioconductor, a repository for R packages related to biological data analysis. To better understand S4 objects as they relate to the Bioconductor core infrastructure. To learn more about a popular Bioconductor S4 Read More...

Here, we will start with the data stored in a Seurat object. For instructions on data import and creating the object, see an Introduction to scRNA-Seq with R (Seurat) . adp

The role of cell clustering is to identify cells with similar transcriptomic profiles by computing Euclidean distances across genes . However, scRNA-Seq experiments include highly dimensional data, with each cell associated with the expression of thousands Read More...

The FindAllMarkers function is particularly useful in identifying the differentially expressed genes that distinguish several groups, such as seen here in the clusters. What makes this unique is that none of the identities are initially Read More...

The Seurat v5 object doesn’t require all assays have the same cells. In this case, Cells() can be used to return the cell names of the default assay while colnames() can be used to Read More...

Now, that we have clusters, we can use differential expression analysis to uncover markers that define our clusters. These markers can be used to assign cell types to our clusters. First, because we are working Read More...

Much like pseudobulk differential expression, the RNA expression can be collapsed into pre-defined components, such as the clusters, if it is believed that cell-to-cell variation is inducing too much confusion in the labeling. This collapsing Read More...

From the paper where this data was obtained, the following (incomplete) list of gene markers was obtained: Mmp3: preadipocytes Mki67: proliferating cells Fabp4: differentiating beige adipocytes and differentiated beige adipocytes Scd1: differentiated beige adipocytes Ucp1: Read More...

Here, we will start with the data stored in a Seurat object. For instructions on data import and creating the object, see an Introduction to scRNA-Seq with R (Seurat) and Getting Started with Seurat: QC Read More...

05/15/2024 - To register to attend, you must log in to your SITC Cancer Immunotherapy CONNECT account. Don&rsquo;t have an account?&nbsp; Create a free one . Join Dr. Karchin of the Johns Read More...

05/02/2024 - To register to attend, you must log in to your SITC Cancer Immunotherapy CONNECT account. Don&rsquo;t have an account?&nbsp; Create a free one . Join Dr. Mullin of the Roswell Read More...

Objectives To explore Bioconductor, a repository for R packages related to biological data analysis. To learn about options for report generation with R: RMarkdown and Quarto. Introducing Bioconductor Bioconductor is both an open source project Read More...

First, notice that you can easily access columns from the sample metadata ( colData() ) using $ . Using brackets to subset: se$SampleName ## [1] GSM1275862 GSM1275863 GSM1275866 GSM1275867 GSM1275870 GSM1275871 GSM1275874 ## [8] GSM1275875 ## 8 Levels: GSM1275862 GSM1275863 GSM1275866 GSM1275867 ... GSM1275875 se$ Read More...

The bulk RNA-Seq test data we've been working with is in FASTQ format. We'd like to do a BLAST search on a couple of these sequences. Data must be in FASTA format to Read More...

This page uses content directly from the Biostar Handbook by Istvan Albert. Always remember to activate the bioinfo environment when working on Biostar class materials. conda activate bioinfo The bulk RNA-Seq test data we've Read More...

The test data consists of two commercially available RNA samples: Universal Human Reference (UHR) and Human Brain Reference (HBR) . The UHR is total RNA isolated from a diverse set of 10 cancer cell lines. The HBR Read More...

The test data consists of two commercially available RNA samples: Universal Human Reference (UHR) and Human Brain Reference (HBR) . The UHR is total RNA isolated from a diverse set of 10 cancer cell lines. The HBR Read More...

This page uses content directly from the Biostar Handbook by Istvan Albert. Obtain RNA-seq test data. The test data consists of two commercially available RNA samples: Universal Human Reference (UHR) and Human Brain Reference (HBR) . Read More...

This page uses content directly from the Biostar Handbook by Istvan Albert. Obtain RNA-seq test data. The test data consists of two commercially available RNA samples: Universal Human Reference (UHR) and Human Brain Reference (HBR) . Read More...

The test data consists of two commercially available RNA samples: Universal Human Reference (UHR) and Human Brain Reference (HBR) . The UHR is total RNA isolated from a diverse set of 10 cancer cell lines. The HBR Read More...

Bioconductor is an R package repository for free open-source software that "facilitates rigorous and reproducible analysis of data from current and emerging biological assays" . Bioconductor is released semi-annually, with two working Bioconductor releases Read More...

A common question posed to the Bioinformatics Training and Education Program (BTEP) is “How can I learn R and Python to analyze my data?”. First, it’s important to state that learning any programming language Read More...

High resolution single cell profiling assays have provided an unprecedented view of many biological systems and processes, but the spatial context in which this biology is occurring is often crucial. Spatial profiling, including spatial transcriptomic Read More...

05/23/2023 - NIH Text Mining and Natural Language Processing SIG is pleased to welcome you to a special event dedicated to Large Language Models. Abstract : The release of ChatGPT and the subsequent launch of GPT-4 by Read More...

The data that we will be working with comes from the airway study that profiled the transcriptome of several airway smooth muscle cell lines under either control or dexamethasone treatment Himes et al 2014 . The dataset Read More...

The data that we will be working with comes from the airway study that profiled the transcriptome of several airway smooth muscle cell lines under either control or dexamethasone treatment Himes et al 2014 . The dataset Read More...

The data that we will be working with comes from the airway study that profiled the transcriptome of several airway smooth muscle cell lines under either control or dexamethasone treatment Himes et al 2014 and the Read More...

Objectives To explore Bioconductor, a repository for R packages related to biological data analysis. To generate high quality data reports using R Markdown to make data analysis more reproducible. Reminder: Uploading files from RStudio Server Read More...

11/02/2022 - Do you need to strengthen your structural biology skills? The NIAID Bioinformatics and Computational Biosciences Branch (BCBB) is offering training sessions over the next few weeks. Current topics include three series of practical training Read More...

10/26/2022 - In partnership with NCI’s Cancer Moonshot SM  Initiative, the Society for Immunotherapy of Cancer (SITC) and the  Big Data  and  Data Sharing  Committee are hosting the eighth of nine webinars that make up Read More...

09/27/2022 - Course Objectives: This 12-week long course is for scientists wanting to learn basic bioinformatics skills. Material will be taught at a beginner to intermediate level. There are no pre-requisites. Learners will: Learn Unix skills Read More...

09/15/2022 - In partnership with the National Cancer Institute (NCI) Cancer Moonshot SM , the Society for Immunotherapy of Cancer (SITC) and the  Big Data and Data Sharing Committee  are excited to announce our second " SITC-NCI Read More...

06/29/2022 - The CCR Genomics, Sequencing and Single Cell Analysis Core Facilities are pleased to host a virtual technology seminar with Illumina. Presentation overview : For decades cancer methylation studies have provided insights into tumorigenic pathways and Read More...

06/14/2022 - In partnership with NCI’s Cancer Moonshot SM  Initiative, the Society for Immunotherapy of Cancer (SITC) and the  Big Data  and  Data Sharing  Committee are hosting the fourth of nine webinars that make up Read More...

05/26/2022 - Want to learn about the latest  data science  technologies and immunotherapy research? In partnership with NCI’s Cancer Moonshot SM  Initiative, the Society for Immunotherapy of Cancer (SITC) and the  Big Data  and  Data Read More...

05/17/2022 - Metabolomic and multi-omic data are increasingly being collected in basic, preclinical, and clinical research studies. Interpretation of these data though remains challenging.  Common challenges include the difficulty in identifying metabolites and assigning unique identifiers, Read More...

04/27/2022 - In partnership with NCI’s Cancer Moonshot SM    Initiative, the Society for Immunotherapy of Cancer (SITC) and the  Big Data  and  Data Sharing  Committee are hosting the second of nine webinars that make up Read More...

05/24/2021 - Dr. Adam Phillippy, a Senior Investigator at NHGRI will give an extremely interesting talk on Monday, 5/24 at 11 am via WebEx at https://nih.webex.com/nih/j.php?MTID=m237a8e77868c23cc4 Read More...

05/03/2021 - Abstract: We will discuss technical advantages of a personalized and tumor-informed multiplex PCR next generation sequencing assay, called Signatera™, that enables a sensitive, specific, and dynamic detection of molecular disease burden in cell-free DNA ( Read More...

12/14/2012 - All researchers at the NCI have unlimited access to Pathway Studio software, MedScan text mining module, and the Mammalian database. Pathway Studio Application Areas Include: • Disease Biology • Disease Biomarkers • Drug Target Identification • Protein Functionality • Read More...

12/11/2012 - All researchers at the NCI have unlimited access to Pathway Studio software, MedScan text mining module, and the Mammalian database. Pathway Studio Application Areas Include: • Disease Biology • Disease Biomarkers • Drug Target Identification • Protein Functionality • Read More...

There are a number of core facilities available to NCI researchers. See more information from the Office of Science and Technology Resources. We most commonly see data from the following cores: CCR Sequencing Facility (CCR-SF) Read More...

There are many packages and functions available in R programming for performing PCA. Some of the most popular functions are stats::prcomp(), stats::princomp(), FactoMineR::PCA(), and ade4::dudi.pca(). These functions largely differ in Read More...

Here, we will download some high throughput genomic sequences from NCBI SRA. The data that we will download were derived from sequencing of the Zaire Ebola virus. See the NCBI SRA page for this study Read More...

Protein Characterization Laboratory (PCL) offers various technologies to CCR investigators to characterize proteins and metabolites. The laboratory develops and applies state-of-the-art analytical technologies, primarily mass spectrometry, liquid chromatography, and Surface Plasmon Resonance (SPR), to advance Read More...

The NHLBI Transgenic Core’s main mission is to keep up with the latest advancements in genome engineering technologies and to provide state-of-the-art services to assist NIH scientists in generating genetically engineered animal models. In Read More...

Total end-to-end system for single-cell research [embed]https://youtu.be/vMzhSzg1rUw[/embed] The BD Rhapsody Single-Cell Analysis system empowers and streamlines your research with a complete system of tools, including reagents and analysis software, Read More...

The Genomics Technology Laboratory is an integrated, high-throughput molecular biology laboratory focusing on the development of genetics and genomics technologies, data analysis, and information management tools, in support of CCR Investigators. The laboratory develops integrated Read More...

The CLIA Molecular Diagnostics Laboratory (CMDL) provides an array of services for groups at the NIH Clinical Center, Fort Detrick, and Hood College, among others. They support cancer- and disease-related research by making Read More...

Protein Characterization Laboratory (PCL) offers various technologies to CCR investigators to characterize proteins and metabolites. The laboratory develops and applies state-of-the-art analytical technologies, primarily mass spectrometry, liquid chromatography, and Surface Plasmon Resonance (SPR), to advance Read More...

What is Visium FFPE v2 with CytAssist? Visium FFPE v2 is sequencing-based spatial profiling technology developed by 10x Genomics. This assay can take mouse or human tissue sections on normal glass slides as input and Read More...

The Antibody Characterization Laboratory (ACL) is the laboratory responsible for the development of well-characterized monoclonal antibody reagents. The NCI’s Office of Cancer Clinical Proteomics Research funds ACL as a resource to the entire cancer Read More...

The NCI High-Throughput Imaging Facility (HiTIF) works in a collaborative fashion with NCI/NIH Investigators by providing them with the necessary expertise, instrumentation, and software to develop and execute advanced High-Throughput Imaging (HTI) assays. These Read More...

The function of the SAIP is to collaborate with NCI investigators in the development of mouse models, new molecular imaging probes for early detection and therapy, monitor tumors in vivo, and perform drug efficacy studies Read More...

The introduction of DNA sequencing instruments capable of producing millions of DNA sequence reads in a single run has profoundly altered the landscape of genetics and cancer biology. Complex questions can now be answered at Read More...

Our operational objectives are to provide state-of-the-art OMICS technologies in support of the Genetics Branch (GB) investigators and collaborators. Research Services Wet Lab Single cell isolation from fresh, frozen, and FFPE tissue, DNA/RNA extractions Read More...

Home About the Biophysics Core Biophysics Core Services [tabby title="Instrumentation"] NHLBI Biophysics Core The Biophysics Core Facility: Overview Core Facilities provide scientific resources, cutting-edge technologies and novel approaches to support DIR scientists. Availability of Read More...

This lesson provides an introduction to R in the context of single cell RNA-Seq analysis with Seurat. Learning Objectives Learn about options for analyzing your scRNA-Seq data. Learn about resources for learning R programming. Learn Read More...

Learning Objectives This tutorial was designed to demonstrate common secondary analysis steps in a scRNA-Seq workflow. We will start with a merged Seurat Object with multiple data layers representing multiple samples. Throughout this tutorial we Read More...

1. Introduction and Learning Objectives This tutorial has been designed to demonstrate common secondary analysis steps in a scRNA-Seq workflow. We will start with a merged Seurat Object with multiple data layers representing multiple samples that Read More...

This page uses content directly from the Biostar Handbook by Istvan Albert. Obtain RNA-seq test data. The test data consists of two commercially available RNA samples: Universal Human Reference (UHR) and Human Brain Reference (HBR) . Read More...

Lesson 1: Introduction to Biowulf, Unix, and R Learning Objectives Learn about why you may want to use R on Biowulf. Refresh Unix and R skills. This lesson will not be hands on. Why use R Read More...

Visualizing clusters with heatmaps Objectives Introduce the heatmap and dendrogram as tools for visualizing clusters in data. Learn to construct cluster heatmap using the package pheatmap . Learn how to save a non-ggplot2 plot. Introduce ggplotify Read More...

Visualizing clusters with heatmaps Objectives Introduce the heatmap and dendrogram as tools for visualizing clusters in data. Learn how to work with the package pheatmap . Learn how to save a non-ggplot2 plot. Introduce ggplotify to Read More...

Visualizing clusters with heatmaps Objectives Introduce the heatmap and dendrogram as tools for visualizing clusters in data. Learn to construct cluster heatmap using the package pheatmap . Learn how to save a non-ggplot2 plot. Introduce ggplotify Read More...

04/17/2017 - Probing DNA-Protein Interactions This 2-day workshop, which includes both lecture and hands-on components, will teach the basic concepts and practical aspects of ChIP-Seq data analysis. Topics to be covered include: experimental design; read alignment; Read More...

The Molecular Signatures Database (MSigDB) is a curated resource of thousands of gene sets by the Broad Institute. These sets were curated for use with GSEA software but are used with other tools as well. Read More...